Bioavailability of phenolic compounds and redox state of murine liver and kidney as sex-dependent responses to phytoextracts

Claudia Albrecht, María Laura Guzmán, María Cecilia Cittadini, Alejandra Mariel Canalis, Mirta Ana Valentich, María Eugenia Olivera, Elio Andrés Soria


Background: Plant extracts can be obtained to carry bioactive compounds, useful for prevention and treatment of different illnesses. This also supports the intake of teas as functional beverages. Nonetheless, it is incompletely known whether these extracts can act as effective sources and vehicles de phenolic compounds (phenolics/polyphenols) to reach their targets. Objective: To establish whether phytoextract ingestion modified in a sex-dependent manner the phenolic bioavailability and redox response in liver and kidney. Method: BALB/C mice ingested for a month 100 mg/Kg/d of extracts (tea-like) from Aspidosperma quebracho-blanco (AQB), Lantana grisebachii (LG) or Ilex paraguariensis (IP). Then, phenolics, peroxides and nitrites were analyzed by spectrophotometry. Also, phenolic permeation from digested and undigested extracts was evaluated in vitro with a rat jejunum-based assay. Results: Phenolic permeation depended on extract digestion. In males, IP showed a special time course of hepatic phenolics, whereas all extracts decreased renal phenolics at 15 days. Extracts induced hepatic lipoperoxides at 15 days. LG reduced renal hydroperoxides at 15 days and hepatic nitrites at 30 days, whereas AQB and IP reduced renal lipoperoxides and nitrites at 30 days. In females, extracts reduced hydroperoxides, with LG and AQB also reducing lipoperoxides. IP increased renal lipoperoxides at 30 days. Conclusion: IP was a relevant phenolic source. Sex-dependent responses were found in all variables, which should be considered to prevent misleading generalizations in phytodrug bioprospecting.

Palabras clave

Quebracho; Ilex paraguariensis; Lantana; Absorption; Oxidation-Reduction

Texto completo:

PDF (English)


Ramos Elizagaray SI, Soria EA. Arsenic immunotoxicity and immunomodulation by phytochemicals: Potential relations to develop chemopreventive approaches. Recent Pat Inflamm Allergy Drug Discov 2014;8: 92-103.

Mitjavila MT, Moreno J. The effects of polyphenols on oxidative stress and the arachidonic acid cascade. Implications for the prevention/treatment of high prevalence diseases. Biochem Pharmacol 2012;84: 1113-1122.

Giri TK, Alexander A, Ajazuddin, Barman TK, Maity S. Infringement of the barriers of cancer via dietary phytoconstituents capsaicin through novel drug delivery system. Curr Drug Deliv 2016;13: 27-39.

Osland EJ, Memon MA. Are we jumping the gun with pharmaconutrition (immunonutrition) in gastrointestinal oncological surgery? World J Gastrointest Oncol 2011;3: 128-130.

Goleniowski ME, Bongiovanni GA, Palacio L, Nuñez CO, Cantero JJ. Medicinal plants from the “Sierra de Comechingones”, Argentina. J Ethnopharmacol 2006;107: 324-341.

Soria EA, Goleniowski ME, Cantero JJ, Bongiovanni GA. Antioxidant activity of different extracts of Argentinian medicinal plants against arsenic-induced toxicity in renal cells. Hum Exp Toxicol 2008;27: 341-346.

Bracesco N, Sanchez AG, Contreras V, Menini T, Gugliucci A. Recent advances on Ilex paraguariensis research: Minireview. J Ethnopharmacol 2011;136: 378-384.

Heitman E, Ingram DK. Cognitive and neuroprotective effects of chlorogenic acid. Nutr Neurosci 2014; 2014:140925080725000.

Veskoukis AS, Tsatsakis AM, Kouretas D. Dietary oxidative stress and antioxidant defense with an emphasis on plant extract administration. Cell Stress Chaperon 2012;17: 11-21.

Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients 2010;2: 1231-1246.

Cittadini MC, Canalis AM, Albrecht C, Soria EA. Effects of oral phytoextract intake on phenolic concentration and redox homeostasis in murine encephalic regions. Nutr Neurosci 2015;18: 316-322.

Murakami A. Dose-dependent functionality and toxicity of green tea polyphenols in experimental rodents. Arch Biochem Biophys 2014:557: 3-10.

Clifford MN, Van der Hooft JJ, Crozier A. Human studies on the absorption, distribution, metabolism, and excretion of tea polyphenols. Am J Clin Nutr 2013;98: 1619S-1630S.

Canalis AM, Cittadini MC, Albrecht C, Soria EA. In vivo redox effects of Aspidosperma quebracho-blanco Schltdl., Lantana grisebachii Stuck and Ilex paraguariensis A. St.-Hil. on blood, thymus and spleen of mice. Indian J Exp Biol 2014;52: 882-889.

Tenore GC, Campiglia P, Giannetti D, Novellino E. Simulated gastrointestinal digestion, intestinal permeation and plasma protein interaction of white, green, and black tea polyphenols. Food Chem 2015;169: 320-326.

Bravo-Osuna I, Vauthier C, Chacun H, Ponchel G. Specific permeability modulation of intestinal paracellular pathway by chitosan-poly(isobutylcyanoacrylate) core-shell nanoparticles. Eur J Pharm Biopharm 2008;69: 436-444.

Clarke LL. A guide to Ussing chamber studies of mouse intestine. Am J Physiol Gastrointest Liver Physiol 2009;296: G1166-G1166.

Soria EA, Bongiovanni GA, Díaz Luján C, Eynard AR. Effect of arsenite on nitrosative stress in human breast cancer cells and its modulation by flavonoids. Nutr Cancer 2015;67: 659-663.

Biasutto L, Mattarei A, Sassi N, Azzolini M, Romio M, Paradisi C, Zoratti M. Improving the efficacy of plant polyphenols. Anticancer Agents Med Chem 2014;14: 1332-1342.

Borneo R, León AE, Aguirre A, Ribotta P, Cantero JJ. Antioxidant capacity of medicinal plants from the Province of Córdoba (Argentina) and their in vitro testing in a model food system. Food Chem 2009;112: 664-670.

Del Rio D, Rodriguez-Mateos A, Spencer JPE, Tognolini M, Borges G, Crozier A. Dietary (poly)phenolics in human health: Structures, bioavailability, and evidence of protective effects against chronic diseases. Antioxid. Redox Signal 2013;18: 1818-1892.

Rivelli DP, Almeida RL, Ropke CD, Barros SBM. Hydrolysis influence on phytochemical composition, antioxidant activity, plasma concentration, and tissue distribution of hydroethanolic Ilex paraguariensis extract components. J Agric Food Chem 2011;59: 8901-8907.

Liu Y, Zhang D, Wu Y, Wang D, Wei Y, et al. Stability and absorption of anthocyanins from blueberries subjected to a simulated digestion process. Int J Food Sci Nutr 2014;65: 440-448.

Makkar HP, Blümmel M, Becker K. Formation of complexes between polyvinyl pyrrolidones or polyethylene glycols and tannins, and their implication in gas production and true digestibility in in vitro techniques. Br J Nutr 1995;73: 897-913.

Patel R, Maru G. Polymeric black tea polyphenols induce phase II enzymes via Nrf2 in mouse liver and lungs. Free Radic Biol Med 2008;44: 1897-1911.

Wen D, Huang X, Zhang M, Zhang L, Chen J, et al. Resveratrol attenuates diabetic nephropathy via modulating angiogenesis. PLoS ONE 2013;8:e82336.

Psotová J, Lasovský J, Vičar J. Metal-chelating properties, electrochemical behavior, scavenging and cytoprotective activities of six natural phenolics. Biomed Pap Med Fac Univ Palacký Olomouc Czech Repub 2003;147: 147-153.

Heck CI, De Mejia EG. Yerba mate tea (Ilex paraguariensis): A comprehensive review on chemistry, health implications, and technological considerations. J Food Sci 2007;72: R138–R151.

Vyas N, Argal A. Diuretic potential of oleanolic acid isolated from Lantana camara. Asian J Pharm Clin Res 2012; 5: S196-S199.

Miller JH, McCoy KD, Colman AS. Renal actions of the α2-adrenoceptor agonist, xylazine, in the anaesthetised rat. N Z Vet J 2001;49: 173-180.

Patwardhan B, Gautam M. Botanical immunodrugs: scope and opportunities. Drug Discov Today 2005;10: 495-502.

Kirby GM, Nichols KD, Antenos M. CYP2A5 induction and hepatocellular stress: An adaptive response to perturbations of heme homeostasis. Curr Drug Metab 2011;12: 186-197.

Wu B, Kulkarni K, Basu S, Zhang S, Hu M. First-pass metabolism via UDP-glucuronosyltransferase: a barrier to oral bioavailability of phenolics. J Pharm Sci 2011;100: 3655-3681.

Waxman DJ, Holloway MG. Sex differences in the expression of hepatic drug metabolizing enzymes. Mol Pharmacol 2009;76: 215-228.

Parimoo HA, Sharma R, Patil RD, Sharma OP, Kumar P, Kumar N. Hepatoprotective effect of Ginkgo biloba leaf extract on lantadenes-induced hepatotoxicity in guinea pigs. Toxicon 2014;81: 1-12.

Zarban A, Toroghi MM, Asli M, Jafari M, Vejdan M, Sharifzadeh G. Effect of vitamin C and E supplementation on total antioxidant content of human breast milk and infant urine. Breastfeed Med 2015;10: 214-217.

Bixby M, Spieler L, Menini T, Gugliucci A. Ilex paraguariensis extracts are potent inhibitors of nitrosative stress: A comparative study with green tea and wines using a protein nitration model and mammalian cell cytotoxicity. Life Sci 2005;77: 345-358.

Korkmaz A, Kolankaya D. Inhibiting inducible nitric oxide synthase with rutin reduces renal ischemia/reperfusion injury. Can J Surg 2013;56: 6-14.

El Gamal AA, Al Said MS, Raish M, Al-Sohaibani M, Al-Massarani SM, Ahmad A. Beetroot (Beta vulgaris L.) extract ameliorates gentamicin-induced nephrotoxicity associated oxidative stress, inflammation, and apoptosis in rodent model. Mediators Inflamm 2014; 2014:983952.

Mustafa AG, Bani-Ahmad MA, Jaradat AQ, Allouh MZ. Tempol protects blood proteins and lipids against peroxynitrite-mediated oxidative damage. Exp Biol Med 2014; 240: 109-112.

Enlaces refback

  • No hay ningún enlace refback.

Copyright (c) 2017 Elio Andrés Soria, Claudia Albrecht, María Laura Guzmán, María Cecilia Cittadini, Alejandra Mariel Canalis, Mirta Ana Valentich, María Eugenia Olivera

Licencia de Creative Commons
Este obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial 4.0 Internacional.

Revista de la Facultad de Ciencias Médicas.
Pabellón Perú - Ciudad Universitaria
Facultad de Ciencias Médicas. Universidad Nacional de Córdoba
ISSN impreso: 0014-6722 | ISSN en linea: 1853-0605