Interstitial cell of the ovaries of the chick embryo: ultraastructural aspects of their innervation
DOI:
https://doi.org/10.31053/1853.0605.v49.n2.45081Keywords:
chick embryo, ovaries, insterstitial cells, innervationAbstract
Iii a previous work wc dernonstrated that germ and epitheiial celis of the atrophic right ovary and of the medulla iii the icft functioning ovary uridergo regression while interstitial celis are preserved. The objective of the prosent study was tu analyze the relationship belween nerve fibers and nerve cnciings aoci interstitial cciis from both ovaries ouring crnbryogenesis in [he chick. To this purpose, lcft and right ovaries from crnbryos of 7 to 19 days of dcvelopment \\'Cre processed for uitrastructira1 study and ultracytochernical determination of cid phosphatasc. At 7 days. isolated interstitial célis were found in the cent ral region O!: hoth ovaries, containin SER, Goigi apparatus, mitochondria wth tubular cristae and abundant lipidic vacuoles. Ncrvc fibers and nerve odings were scarce. From the lltday, interstitial ccils vcie founc1 grouped in the right ovary aoci jo the medulia of the !cft one, and were related lo nerve bundies surroundcd by Schwann celis. The nerve encling, jo cjose contact with the membrane of interstitial celis, container! mifochon- dna, microtubulcs aoci sma]i ami big 'esicles, sorne with ari eiectrodensc con- tent. This reiationship betwecn inters titial celis and nerve endings and fibers was more frequent from the 15th day. The activitv of acid p1iosphatase locali-
zed in gerrn and cpitheiial cells pre- cnteci a rnorc' intense rcaction jo the right ovary and in the niedulia of thc' Jeft ovary at 15 days. Enzyrne activity was not detected in int.erstitini celis nor íri nerve endings and fibers al: the cliffc- runt agcs studied. Thesc results suggest that nerve fibers and nerve endings vouid ix' necessary for interstitial celis dil[ercntiation in both ovaries of the chick crnbryo.
Downloads
References
AlflauuioL. A.; Yasuada, K.: 1nnerviti,in of the ovarian interstitial celis of the chick embryo. Experientia, 35 (3):; 403- 404, 1979.
Amanurna, A.; Yamada, K.: The innervation of interstitial ceil of the estrogen treated male gonads la chick embryo. J Electron Microsc., 29 (2): 183-185, 1980.
Avila, R.E.; Sainar, M.E.; Fabro, S.P. de: Ultrastructural changes of left and right ovaries from chick embryo. Comun Biol, 6 (1): 83-72, 1987.
Avila, RE.; Sainar, M.E.; Fabro, S.P. de: Desarrollo in vitro de las gónadas femeninas del embrión de pollo. Rey. Fac. Ciencias M&1, Córdoba XLV: 13-24, 1987.
Avila, R.E.; Fabro, S. P. de: Hormone induced differentiation of ovaries from chick embryo in tissue culture. Comun Biol, 8 (2): 121-133, 1989.
Dahl, E.: Studies of the fine structure of ovarian interstitial tissue. The innervation of the thecal gland of the domestic fowi. Z. Zellforsch, 109 (2): 212-226, 1970.
Dahl, E.: Studies on the fine structure ol ovarian interstitial tissue. Efects of go- naclotropins on the thecal gland of the domestie fowi. Z. Zellforsch, 113: 138- 156, 1971.
De Sirnone Saiitoro, 1.: L'organogenesi dell ovaio ensbrionale di pollo. (Ricerche utrastructurali ) . Biol. Lat., 20 (2): 137- 186, 1967.
De Simone Santoio, 1.: UltrastructuraL aspeel 01 the interstitial ovarian cells ni the chick embryo, Bou. Soc. Ital, Sper., 43 (15): 908-910, 1967.
De Simone Sautoro, 1.: lorpJiologie ulIraestiucturale de lovaire de 1'embryon de poioet au cours de sa rlifférenciatioa et de son organogenése. J. Micros., 8: 739-752, 1969.
Case, 1. M.: Estrogen target celis in gonads of the chicken embryo during sexual differentiation. j. Embrvol Exp Morphol, 55: 331-342, 1980.
Guraya, S.: Recent advances in the mor phology, histochemistry and biochemistry of steroid-synthesizing cellular sites ja the nonmarnmalian vertebrate ovarv. mt Rey Cytol, 44: 365-409, 1976.
1-lamilton, H.; Hamburger, V.; A series of normal stages in the development of the chick ernbryos. J. Morphol. 18: 49- 92, 1951.
iones, G.J.; Fox, 1-1.: An ultrahistochernical study of the distribution of acid, alkaune phosphatases in placentae from nor- mal, complicated pregnancies. J. Path, 118: 143-145, 1976.
Jordanov, J.. Angelova, P.: Ultrastructure of developing interstitial cells in chick ernbryonic gonads in relation to their genesis and stcroidogenic function. Z. Mikrosk, Anat. Forsch. Leipzing. 92: 449-464, 1978.
Jordanov, J.; Angelova, P.: Sorne rejationships among gerrn, satellite and interstitial cells during chick gonad differentiation: A tissue culture study. Ann. Biol. Anim. Biochem. Biophys., 19 (43) 12791288, 1979.
Karnovsky, M .J.: A formaklehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Ce!! Biol. 27: 137A, 1965.
Narbaitz, 11.; De Robertis, E.: Steroid producing celis in chick intersexual gonads. Gen. Comp. Endoc. 41 (1): 164- 169, 1970.
Owen, J . T . P. : Karyotype studies on gaItus domesticus. Chrornosoma (Ben) 16: 601-608, 1965.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Universidad Nacional de Córdoba
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The generation of derivative works is allowed as long as it is not done for commercial purposes. The original work may not be used for commercial purposes.
How to Cite
