Differential histopathological response of pepper (Solanaceae) cultivars to Nacobbus celatus (Nematoda) populations
DOI:
https://doi.org/10.31055/1851.2372.v59.n2.44120Keywords:
Capsicum annum, defence mechanism, false root-knot nematode, histology, syncytiumAbstract
Background and aims: The false-root knot nematode (Nacobbus spp.) comprises polyphagous species, with pepper (Capsicum annuum) being one of the most affected crops. Due to the lack of resistant pepper genotypes, we compared the responses of different commercial cultivars to identify possible plant defence mechanisms against N. celatus.
M&M: Nematodes from Río Cuarto (Córdoba Province) and Lisandro Olmos (Buenos Aires Province) were inoculated on pepper cultivars: California Wonder (control), Fyuco INTA, Yatasto, and Fenomeno RZ. Plants were grown under a greenhouse and galls were analysed by histopathological techniques.
Results: The roots of the commercial cultivars showed the typical morpho-anatomical alterations induced by N. celatus: gall formation, hyperplasia reactions, development of syncytia in the central cylinder and disorganisation of vascular tissues. Syncytial cells in contact with females of nematodes from Lisandro Olmos showed a defence reaction by Fyuco INTA, whereas feeding sites in Fenomeno RZ did not denote high metabolic activity in nematodes of both origins.
Conclusions: The different responses observed between populations and/or hosts demonstrate the importance of considering nematodes from different geographical origins when testing plant material for tolerance and/or resistance to N. celatus. Studies of this nature are necessary since they facilitate a more in-depth understanding of the parasite-plant interaction.
References
BARBOZA, G. E., C. C. GARCÍA, L. DE BEM BIANCHETTI, M. V. ROMERO & M. SCALDAFERRO. 2022. Monograph of wild and cultivated chili peppers (Capsicum L., Solanaceae). PhytoKeys 200: 1-423. https://doi.org/10.3897/phytokeys.200.71667
BENTES, J. L. S & K. MATSUOKA. 2005. Histologia da interação Stemphylium solani e tomateiro. Fitopatol. Bras. 30: 224-231.
https://doi.org/10.1590/S0100-41582005000300002
CABRERA, V. A., N. DOTTORI & M. E. DOUCET. 2017. Histopathology of roots of three tomato cultivars infected with two separate isolates of the false root-knot nematode Nacobbus aberrans. Eur. J. Plant Pathol. 148: 393-403.
https://doi.org/10.1007/s10658-016-1097-1
CONN, H. J., M. A. DARROW & V. M. EMMEL. 1960. Staining procedures. Williams Wilkins Co., Baltimore.
DAS, S., D. A. DEMASON, J. D. EHLERS, T. J. CLOSE & P. A. ROBERTS. 2008. Histological characterization of root-knot nematode resistance in cowpea and its relation to reactive oxygen species modulation. J. Exp. Bot. 59: 1305-1313. https://doi.org/10.1093/jxb/ern036
EPPO (European and Mediterranean Plant Protection Organization). 2024. EPPO A1 list of pests recommended for regulation as quarantine pests. Available from: https://www.eppo.int/ACTIVITIES/plant_quarantine/A1_list#nematodes [Accessed: 9 January 2024]
EVES-VAN DEN AKKER, S., C. J. LILLEY, E. G. DANCHIN, C. RANCUREL, …, & J. T. JONES. 2014. The transcriptome of Nacobbus aberrans reveals insights into the evolution of sedentary endoparasitism in plant-parasitic nematodes. Genome Biol. Evol: 6: 2181-2194. https://doi.org/10.1093/gbe/evu171
FAO. 2022. FAOSTAT: Cultivos y productos de ganadería. Available from: https://www.fao.org/faostat/es/#data/QCL [Accessed: 9 January 2024]
HERNÁNDEZ-LÓPEZ, J., A. TOVAR-SOTO, A. CARVAJAL-SANDOVAL & R. TORRES-CORONEL. 2006. Alteraciones anatómicas inducidas por Cactodera galinsogae (Nemata: Heteroderinae) en miembros de Asteraceae. Polibotánica 22: 1-8.
JONES, M. G. & H. L. PAYNE. 1977. The structure of syncytia induced by the phytoparasitic nematode Nacobbus aberrans in tomato roots, and the possible role of plasmodesmata in their nutrition. J. Cell Sci. 23: 299-313. https://doi.org/10.1242/jcs.23.1.299
LAX, P., M. E. DOUCET, R. BRAGA & R. GIORIA. 2006. Response of different pepper varieties to the attack by two populations of Nacobbus aberrans. Nematol. Bras. 30: 259-265.
LAX, P., J. C. R. DUEÑAS, N. B. CORONEL, C. N. GARDENAL, ..., & M. E. DOUCET. 2011. Host range study of Argentine Nacobbus aberrans sensu Sher populations and comments on the differential host test. Crop Prot. 30: 1414-1420. https://doi.org/10.1016/j.cropro.2011.06.001
LAX, P., M. TORDABLE, J. MACAGNO, P. BIMA & M. E. DOUCET. 2013. Response of different potato cultivars to the presence of Nacobbus aberrans. Nematropica 43: 83-90.
LAX, P., J. C. R. DUEÑAS, D. RAMOS, M. E. DOUCET, R. BRAGA & R. KOBORI. 2016. Host suitability of peppers to the false root-knot nematode Nacobbus aberrans. Crop Prot. 79: 15-19. https://doi.org/10.1016/j.cropro.2015.09.024
LAX, P., R. E. GONZALEZ‐ITTIG, J. C. RONDAN DUEÑAS, A. J. ANDRADE, …, & M. E. DOUCET. 2021. Decrypting species in the Nacobbus aberrans (Nematoda: Pratylenchidae) complex using integrative taxonomy. Zool. Scr. 50: 667-688. https://doi.org/10.1111/zsc.12494
LAX, P., M. A. PASSONE, A.G. BECERRA, A. L. SOSA, …, & L. C. ROSSO. 2022. Sustainable strategies for management of the “false root-knot nematode” Nacobbus spp. Front. Plant Sci. 13: 1046315.
https://doi.org/10.3389/fpls.2022.1046315
LEVIN, K. A., M. R. TUCKER, D. M. BIRD & D. E. MATHER. 2020. Infection by cyst nematodes induces rapid remodelling of developing xylem vessels in wheat roots. Sci. Rep. 10: 1-9. https://doi.org/10.1038/s41598-020-66080-z
LOMELÍ-ROSARIO, M. G., A. CARVAJAL-SANDOVAL, R. TORRES-CORONEL, J. M. HERNÁNDEZ- LÓPEZ & A. TOVAR-SOTO. 2009. Alteraciones anatómicas e histológicas inducidas por Meloidogyne incognita (Nematoda: Heteroderidae) en raíces del arbusto urbano arrayán (Buxus sempervirens L.). En: ORTEGA REYES, J., J. E. SEDEÑO DÍAZ & E. LÓPEZ LÓPEZ (eds.), Setenta y cinco años de la Escuela Nacional de Ciencias Biológicas, pp. 304-311. Instituto Politécnico Nacional, México.
LOPES, C. M. L., N. D. SUASSUNA, J. E. CARES, A. C. M. M. GOMES, …, & R. M. D. G. CARNEIRO. 2020. Marker-assisted selection in Gossypium spp. for Meloidogyne incognita resistance and histopathological characterization of a near immune line. Euphytica 2162: 1-15. https://doi.org/10.1007/s10681-020-2554-7
MANZANILLA-LÓPEZ, R. H., M. A. COSTILLA, M. DOUCET, J. FRANCO, …, & K. EVANS. 2002. The genus Nacobbus Thorne & Allen, 1944 (Nematoda: Pratylenchidae): systematics, distribution, biology and management. Nematropica 32: 149-228.
MOYETTA, N., P. LAX, R. BRAGA, R. GIORIA & M. DOUCET. 2007. Histopatología en raíces de cultivares experimentales y comerciales de pimiento (Solanaceae) atacados por una población de Nacobbus aberrans (Nematoda: Tylenchida) procedente de Catamarca. Kurtziana 33: 39-47.
PARVEZ, G. M. 2017. Current advances in pharmacological activity and toxic effects of various Capsicum species. Int. J. Pharm. Sci. Res. 8: 1900-1912.
http://dx.doi.org/10.13040/IJPSR.0975-8232.8(5).1900-12
PETITOT, A. S., T. KYNDT, R. HAIDAR, A. DEREEPER & D. FERNANDEZ. 2017. Transcriptomic and histological responses of African rice (Oryza glaberrima) to Meloidogyne graminicola provide new insights into root-knot nematode resistance in monocots. Ann. Bot. 119: 885-899. https://doi.org/10.1093/aob/mcw256
ROSSO, L., A. DE CANDIA, P. LEONETTI & A. CIANCIO. 2004. Alteraciones histopatológicas causadas por Meloidogyne incognita en almendro (Prunus amygdalus). Nematropica 34: 257-261.
TORDABLE, M. D. C., P. LAX & M. E. DOUCET. 2007. Histopatología de raíces de pimiento y remolacha atacadas por dos poblaciones del nematodo fitófago Nacobbus aberrans de Córdoba. En: VI Encuentro Nacional Científico Técnico de Biología del suelo. VI Encuentro sobre Fijación Biológica del Nitrógeno. Universidad Nacional de Río Cuarto Río Cuarto.
TORDABLE, M. D. C., P. LAX, M. E. DOUCET, P. BIMA, … & L. VARGAS. 2010. Respuesta de raíces de diferentes plantas a la presencia del falso nematodo agallador Nacobbus aberrans. Russ. J. Nematol. 18: 31-39.
TORDABLE, M. D. C., P. LAX, M. E. DOUCET, O. LUQUE & N. ROJAS. 2010. Histopathological study in Salsola kali roots infected by Nacobbus aberrans. Nematropica 40: 105-110.
TORDABLE, M. D. C., A. J. ANDRADE, M. E. DOUCET & P. LAX. 2018. Histopathology of Andean Potato (Solanum tuberosum Andigenum group) varieties parasitized by the false root-knot nematode, Nacobbus aberrans. Braz. J. Biol. 78: 679-685.
https://doi.org/10.1590/1519-6984.172401
TOULET, M. L., D. A. NEIRA, M. ESCOBAR, E. M. PARDO, …, & N. R. CHALFOUN. 2022. Morphological and pathogenic characterization of Corynespora cassiicola isolates reveals specific genotypic interactions in soybean. Plant Pathol. 71: 843-859. https://doi.org/10.1111/ppa.13528
VOVLAS, N., A. I. NICO, F. DE LUCA, C. DE GIORGI & P. CASTILLO. 2007. Diagnosis and molecular variability of an Argentinean population of Nacobbus aberrans with some observations on histopathology in tomato. J. Nematol. 39: 17-26.
ZARLAVSKY, G. 2014. Histología Vegetal. Técnicas simples y complejas. Sociedad Argentina de Botánica, Buenos Aires.
Published
Issue
Section
License
Copyright (c) 2024 Verónica A. Cabrera, Marcelo E. Doucet, Paola Lax
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Provides immediate and free OPEN ACCESS to its content under the principle of making research freely available to the public, which fosters a greater exchange of global knowledge, allowing authors to maintain their copyright without restrictions.
Material published in Bol. Soc. Argent. Bot. is distributed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International license.
