Detección de especies de Helicobacter en humanos y sus perros

Corina Guendulain; Pablo Tamiozzo; Griselda González; Marina Caffaratti

doi:10.31053/1853.0605.v80.n3.36769

Authors

Corina Guendulain National University of Rio Cuarto https://orcid.org/0000-0002-8634-460X
Pablo Tamiozzo National University of Rio Cuarto https://orcid.org/0000-0002-4501-9026
Griselda González National University of Rio Cuarto
Marina Caffaratti National University of Rio Cuarto https://orcid.org/0000-0001-9013-2696

DOI:

https://doi.org/10.31053/1853.0605.v80.n3.36769

Keywords:

Helicobacter, stomach, veterinary public health

Abstract

Helicobacter is a genus of Gram-negative bacteria that colonizes the stomach of humans and dogs, among other mammals. The most frequent species found in the gastric mucosa of man is H. pylori, however, there are other species of Helicobacter in the stomach of humans, such as H. suis, H. bizzozeronii, H. felis, H. salomonis and H. heilmannii sensu stricto, named non H. pylori Helicobacter (NHPH) some of which also colonize the dog's stomach. The detection of NHPH in the stomach mucosa of humans with gastric disease, and in a high percentage of dogs, suggests the possibility that these animals play an important role in the pathogenesis and transmission of infection to man, postulating the path of transmission oral-oral or fecal-oral. The aim of this study was to determine whether people with signs of chronic gastritis who have Helicobacter spp. and who own dogs as pets, share the same species with them. The presence of Helicobacter spp. in gastric samples of 30 people with clinical signs of chronic gastritis and histopathological diagnosis of gastric infection with Helicobacter spp. was analyzed by PCR. The presence of Helicobacter spp. was also studied in the dogs of these people. Then, the species of Helicobacter presented was determined in both. In human gastric samples, H. pylori was the only species found, while in dogs, samples were H. bizzozeronii, H. felis, H. salomonis and H. heilmannii. In this work the same species were not found in dogs and their owners.

Author Biographies

Corina Guendulain, National University of Rio Cuarto

Professor of Small Animal Clinic, Animal Clinic Department, Faculty of Agronomy and Veterinary Medicine. National University of Rio Cuarto. Specialist in Medical Clinic for Dogs and Cats, Doctor in Health Sciences.
Pablo Tamiozzo, National University of Rio Cuarto

Professor of Transmissible and Toxic Diseases of Pigs, Pathology Department, Faculty of Agronomy and Veterinary Medicine. National University of Rio Cuarto. Doctor in Animal Science from the National University of the Center of the Province of Buenos Air
Griselda González, National University of Rio Cuarto

Professor of Small Animal Clinic, Animal Clinic Department, Faculty of Agronomy and Veterinary Medicine. National University of Rio Cuarto. Specialist in Veterinary Clinical Sciences, Doctor in Veterinary Sciences. Director of the Specialization in Medical Clinic for Dogs and Cats, UNRC.
Marina Caffaratti, National University of Rio Cuarto

Teacher, Head of Practical Work, Small Animal Clinic, Animal Clinic Department, Faculty of Agronomy and Veterinary Medicine. National University of Rio Cuarto. Specialist in Medical Clinic for Dogs and Cats.

References

1. Haesebrouck F, Pasmans F, Flahou B, Chiers K, Baele M, Meyns T, Decostere A, Ducatelle R. Gastric helicobacters in domestic animals and nonhuman primates and their significance for human health. Clin Microbiol Rev. 2009 Apr;22(2):202-23, Table of Contents. doi: 10.1128/CMR.00041-08.

2. Hooi JKY, Lai WY, Ng WK, Suen MMY, Underwood FE, Tanyingoh D, Malfertheiner P, Graham DY, Wong VWS, Wu JCY, Chan FKL, Sung JJY, Kaplan GG, Ng SC. Global prevalence of Helicobacter pylori infection: systematic review and meta-analysis. Gastroenterology. 2017 Aug;153(2):420-429. doi: 10.1053/j.gastro.2017.04.022

3. Sarem M, Corti R. Rol de las formas cocoides de Helicobacter pylori en la infección y la recrudescencia. Gastroenterol Hepatol. 2016 Jan;39(1):28-35. doi: 10.1016/j.gastrohep.2015.04.009.

4. Matos R, Amorim I, Magalhães A, Haesebrouck F, Gärtner F, Reis CA. Adhesion of Helicobacter species to the human gastric mucosa: a deep look into glycans role. Front Mol Biosci. 2021 May 7;8:656439. doi: 10.3389/fmolb.2021.656439.

5. Øverby A, Murayama SY, Michimae H, Suzuki H, Suzuki M, Serizawa H, Tamura R, Nakamura S, Takahashi S, Nakamura M. Prevalence of gastric Non-Helicobacter pylori-Helicobacters in japanese patients with gastric disease. Digestion. 2017;95(1):61-66. doi: 10.1159/000452400.

6. Zamani M, Ebrahimtabar F, Zamani V, Miller WH, Alizadeh-Navaei R, Shokri-Shirvani J, Derakhshan MH. Systematic review with meta-analysis: the worldwide prevalence of Helicobacter pylori infection. Aliment Pharmacol Ther. 2018 Apr;47(7):868-876. doi: 10.1111/apt.14561.

7. Priestnall SL, Wiinberg B, Spohr A, Neuhaus B, Kuffer M, Wiedmann M, Simpson KW. Evaluation of "Helicobacter heilmannii" subtypes in the gastric mucosas of cats and dogs. J Clin Microbiol. 2004 May;42(5):2144-51. doi: 10.1128/JCM.42.5.2144-2151.2004.

8. Van den Bulck K, Decostere A, Baele M, Driessen A, Debongnie JC, Burette A, Stolte M, Ducatelle R, Haesebrouck F. Identification of non-Helicobacter pylori spiral organisms in gastric samples from humans, dogs, and cats. J Clin Microbiol. 2005 May;43(5):2256-60. doi: 10.1128/JCM.43.5.2256-2260.2005.

9. Amorim I, Smet A, Alves O, Teixeira S, Saraiva AL, Taulescu M, Reis C, Haesebrouck F, Gärtner F. Presence and significance of Helicobacter spp. in the gastric mucosa of Portuguese dogs. Gut Pathog. 2015 Apr 16;7:12. doi: 10.1186/s13099-015-0057-1.

10. Guerra Segundo DD, Mello CBE, Cargnelutti JF, Flores MM, Pedrotti LF, Antunes BN, Milech V, Velasquez OG, Martins LR, Pinto Filho STL. Evidence of Helicobacter spp. in saliva and gastric mucosa of domestic dogs in the central region of Rio Grande do Sul, Brazil. Vet Med Int. 2021 Jan 28;2021:8857231. doi: 10.1155/2021/8857231.

11. Husnik R, Klimes J, Kovarikova S, Kolorz M. Helicobacter Species and their association with gastric pathology in a cohort of dogs with chronic gastrointestinal signs. Animals (Basel). 2022 May 13;12(10):1254. doi: 10.3390/ani12101254.

12. Buczolits S, Hirt R, Rosengarten R, Busse HJ. PCR-based genetic evidence for occurrence of Helicobacter pylori and novel Helicobacter species in the canine gastric mucosa. Vet Microbiol. 2003 Sep 24;95(4):259-70. doi: 10.1016/s0378-1135(03)00182-2.

13. Astudillo Vergara M, Detección de Helicobacter pylori y organismos Helicobacter heilmannii-like en mucosa gástrica de perros, a través de la reacción en cadena de la polimerasa (PCR). Tesis de grado, Universidad de Chile. 2009, 55 pp. Disponible en: https://repositorio.uchile.cl/handle/2250/131434

14. Ülgen S, Ergin S, Şennazli G, Bakirel U. Detection of Helicobacter heilmannii type II and Helicobacter pylori in dogs and their role in the development of gastritis. Turk J Vet Anim Sci. 2016; 40 (1):81-8. doi: 10.3906/vet-1502-82.

15. Thomson MA, Storey P, Greer R, Cleghorn GJ. Canine-human transmission of Gastrospirillum hominis. Lancet. 1994 Jun 25;343(8913):1605-7. doi: 10.1016/s0140-6736(94)93060-0.

16. De Bock M, Van den Bulck K, Hellemans A, Daminet S, Coche JC, Debongnie JC, Decostere A, Haesebrouck F, Ducatelle R. Peptic ulcer disease associated with Helicobacter felis in a dog owner. Eur J Gastroenterol Hepatol. 2007 Jan;19(1):79-82. doi: 10.1097/01.meg.0000221856.19201.33.

17. Guendulain C, González G, Pelliza B, Caffaratti M, Bessone A, Tamiozzo P. Detección de Helicobacter bizzozeronii y Helicobacter felis en biopsias gástricas de perros. Rev Cient. 2016;26(5):286-92. Disponible en: https://www.redalyc.org/articulo.oa?id=95949758004

18. Al-Soud WA, Bennedsen M, On SLW, Ouis IS, Vandamme P, Nilsson HO, Ljungh Å, Wadström T. Assessment of PCR-DGGE for the identification of diverse Helicobacter species, and application to faecal samples from zoo animals to determine Helicobacter prevalence. J Med Microbiol. 2003 Sep;52(Pt 9):765-771. doi: 10.1099/jmm.0.05314-0.

19. Goto K, Ohashi H, Takakura A, Itoh T. Current status of Helicobacter contamination of laboratory mice, rats, gerbils, and house musk shrews in Japan. Curr Microbiol. 2000 Sep;41(3):161-6. doi: 10.1007/s002840010111.

20. Bickley J, Owen RJ, Fraser AG, Pounder RE. Evaluation of the polymerase chain reaction for detecting the urease C gene of Helicobacter pylori in gastric biopsy samples and dental plaque. J Med Microbiol. 1993 Nov;39(5):338-44. doi: 10.1099/00222615-39-5-338.

21. Brisou P, Courcoux P, Labigne A. Detection of Helicobacter pylori by polymerase chain reaction. Rev Esp Enferm Dig. 1990; 78 (Suppl 1) 30 (45) P-50.

22. Baele M, Van den Bulck K, Decostere A, Vandamme P, Hänninen ML, Ducatelle R, Haesebrouck F. Multiplex PCR assay for differentiation of Helicobacter felis, H. bizzozeronii, and H. salomonis. J Clin Microbiol. 2004 Mar;42(3):1115-22. doi: 10.1128/JCM.42.3.1115-1122.2004.

23. De Groote D, van Doorn LJ, Ducatelle R, Verschuuren A, Haesebrouck F, Quint WG, Jalava K, Vandamme P. 'Candidatus Helicobacter suis', a gastric Helicobacter from pigs, and its phylogenetic relatedness to other gastrospirilla. Int J Syst Bacteriol. 1999 Oct;49 Pt 4:1769-77. doi: 10.1099/00207713-49-4-1769.

24. Flahou B, Modrý D, Pomajbíková K, Petrželková KJ, Smet A, Ducatelle R, Pasmans F, Sá RM, Todd A, Hashimoto C, Mulama M, Kiang J, Rossi M, Haesebrouck F. Diversity of zoonotic enterohepatic Helicobacter species and detection of a putative novel gastric Helicobacter species in wild and wild-born captive chimpanzees and western lowland gorillas. Vet Microbiol. 2014 Nov 7;174(1-2):186-94. doi: 10.1016/j.vetmic.2014.08.032.

25. R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. 2013 Disponible en: http://www.R-project.org/.

26. Solnick JV, Schauer DB. Emergence of diverse Helicobacter species in the pathogenesis of gastric and enterohepatic diseases. Clin Microbiol Rev. 2001 Jan;14(1):59-97. doi: 10.1128/CMR.14.1.59-97.2001.

27. Wiinberg B, Spohr A, Dietz HH, Egelund T, Greiter-Wilke A, McDonough SP, Olsen J, Priestnall S, Chang YF, Simpson KW. Quantitative analysis of inflammatory and immune responses in dogs with gastritis and their relationship to Helicobacter spp. infection. J Vet Intern Med. 2005 Jan-Feb;19(1):4-14.

28. Neiger R, Tschudi M, Burnens AP, Burkard G, Schmassmann A. Diagnosis and identification of gastric Helicobacter species by PCR in dogs. Microb Ecol Health Dis. 1999;11(4):234-40.

29. Hwang CY, Han HR, Youn HY. Prevalence and clinical characterization of gastric Helicobacter species infection of dogs and cats in Korea. J Vet Sci. 2002 Jun;3(2):123-33.