Development of microsatellite markers for the American species Vachellia aroma (Fabac

Autores

  • Carolina L. Pometti Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Instituto IEGEBA (CONICET- UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina https://orcid.org/0000-0003-0122-6338
  • Cecilia F. Bessega Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Instituto IEGEBA (CONICET- UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina https://orcid.org/0000-0002-8575-1828
  • Mauricio Ewens Estación Experimental Fernández-UCSE (Convenio Provincia Santiago del Estero-Universidad Católica Santiago del Estero), Santiago del Estero, Argentina https://orcid.org/0000-0002-7499-914X
  • Juan C. Vilardi Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Instituto IEGEBA (CONICET- UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina https://orcid.org/0000-0002-7247-7911
  • Beatriz O. Saidman Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Instituto IEGEBA (CONICET- UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina https://orcid.org/0000-0003-4704-3713

DOI:

https://doi.org/10.31055/1851.2372.v59.n1.40763

Palavras-chave:

454 pyrosequencing, polymorphic SSR, Vachellia aroma,, Vachellia caven

Resumo

Background and aims: There are currently no microsatellite markers available for any American species of Vachellia, and particularly, for V. aroma. Then the aims of this study were to develop SSR markers specific for V. aroma, for the first time, and test its amplification in a close related species.

M&M: For the development of the SSR in V. aroma, total genomic DNA was extracted and it was sequenced in a one-fourth run on a Roche 454 GS FLX+ platform. The study area included two Argentinean populations: San José and Robles, Santiago del Estero province. 

Results: We detected 422 sequences containing SSR loci. A set of 39 primer pairs presented amplified products in V. aroma and V. caven, but 12 revealed clear, replicable and polymorphic loci in V. aroma.

Conclusions: The results of this work indicate that a new set of SSR markers was developed for V. aroma and their transferability to V. caven was assessed. The analysis of variability, showed that these 12 polymorphic markers are highly informative, and a powerful tool to investigate population genetics parameters in V. aroma and related species.

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Publicado

2024-03-28

Como Citar

Pometti, Carolina L., Cecilia F. Bessega, Mauricio Ewens, Juan C. Vilardi, e Beatriz O. Saidman. 2024. “Development of Microsatellite Markers for the American Species Vachellia Aroma (Fabac”. Boletín De La Sociedad Argentina De Botánica 59 (1). https://doi.org/10.31055/1851.2372.v59.n1.40763.

Edição

v. 59 n. 1 (2024): Marzo

Seção

Genética & Evolución

Licença

Copyright (c) 2024 Carolina Luciana Pometti, Cecilia Bessega, Mauricio Ewens, Juan C. Vilardi, Beatriz O. Saidman

Creative Commons License

Este trabalho está licenciado sob uma licença Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Proporciona ACESSO ABERTO imediato e livre ao seu conteúdo sob o princípio de tornar a pesquisa livremente disponível ao público, o que promove uma maior troca de conhecimento global, permitindo que os autores mantenham seus direitos autorais sem restrições.

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