Estructura del nectario floral, presentación del néctar y análisis morfo-anatómico de anteras de líneas androfértiles y androestériles en cebolla (Allium cepa, Amaryllidaceae)

Autores/as

DOI:

https://doi.org/10.31055/1851.2372.v58.n3.38251

Palabras clave:

anteras, bolsillo nectarífero, cebolla, dehiscencia, nectario ginopleural, nectario septal.

Resumen

Introducción y objetivos: La cebolla (Allium cepa var. cepa) es una especie alógama, donde se han desarrollado líneas androestériles. La estructura floral y la de sus nectarios florales ha sido descrita de manera somera e incompleta. El objetivo del presente trabajo es actualizar los datos de la anatomía floral comparando líneas fértiles y androestériles y analizar la estructura tridimensional del nectario floral. 

M&M: Se utilizaron técnicas convencionales de microscopía óptica y electrónica de barrido. 

Resultados: Las flores fértiles y androestériles presentan la misma organización estructural. El nectario septal o ginopleural se organiza en tres zonas: 1) área productora formada por el tejido glandular dispuesto radialmente en los septos del ovario, 2) zona de descarga: un pequeño canal en el ápice conecta el tejido nectarífero con el bolsillo formado entre la pared del ovario y la cresta que los cubre, y 3) zona de presentación y cosecha, donde el néctar excretado se acumula, formada entre la base ensanchada de los filamentos estaminales internos y la base del ovario. 

Conclusiones: Las únicas diferencias anatómicas en las flores de las líneas androestériles y las fértiles radican en la falta de producción de granos de polen y la ausencia de dehiscencia, pese a presentar estomio y pared de anteras normales. Los nectarios florales de la cebolla son un claro ejemplo de presentación secundaria del néctar.

Referencias

ABROL, D. 2005. Pollination Energetics. J. Asia Pac. Entomol. 8: 3-14. https://doi.org/10.1016/S1226-8615(08)60066-7

AHMAD, R., M. U. HASSAN, G. B. AKHTAR, S., SAEED, S. A. KHAN, M. K. N. SHAH & N. KHAN. 2020. Identification and characterization of important sterile and maintainer lines from various genotypes for advanced breeding programmes of onion (Allium cepa). Plant Breed. 139: 988-995. https://doi.org/10.1111/pbr.12844

BERNARDELLO, G. 2007. A systematic survey of floral nectaries. In: NICOLSON, S. W., M. NEPI & E. PACINI (eds.), Nectaries and nectar, pp. 19-128. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-5937-7_2

BONNER, L. & H. DICKINSON. 1989. Anther dehiscence in Lycopersicon esculentum Mill. New Phytol. 113: 97-115. https://doi.org/10.1111/j.1469-8137.1989.tb02399.x

BREWSTER, J. L. 2001. Las cebollas y otros alliums. Editorial Acribia, Zaragoza.

CESPEDES, M., PERALTA, I. & C. R. GALMARINI. 2004. Relación entre la morfología de la flor de cebolla y la frecuencia de visitas de abejas polinizadoras. Horticultura Argentina 23: 65.

CHOI, H. J., A. R. DAVIS & J. H. COTA-SÁNCHEZ. 2011. Comparative floral structure of four new world Allium (Amaryllidaceae) species. Syst. Bot. 36: 870-882.

https://doi.org/10.1600/036364411X604895

CHOI, H. J., L. M. GIUSSANI, C. G. JANG, B. U. OH & J. H. COTA-SÁNCHEZ. 2012. Systematics of disjunct northeastern Asian and northern North American Allium (Amaryllidaceae). Botany 90: 491-508. https://doi.org/10.1139/b2012-031

COLOMBO, N. & C. R. GALMARINI. 2017. The use of genetic, manual and chemical methods to control pollination in vegetable hybrid seed production: a review. Plant Breed. 136: 87-299. https://doi.org/10.1111/pbr.12473

CURRAH, L. & D. J. OCKENDON. 1978. Protandry and the sequence of lower opening in the onion (Allium cepa L.). New Phytol. 81: 419-428. https://doi.org/10.1111/j.1469-8137.1978.tb02647.x

DI FULVIO, T. E. 1973. Sobre el gineceo de Allium y Nothoscordum. Kurtziana 7: 241-253.

DIVIJA, S. D. & P. D. KAMALA JAYANTHI. 2022. Pollination efficiency and foraging behaviour of honey bees and flies to onion Allium cepa L. J. Apicult. Res. 61: 688-694. https://doi.org/10.1080/00218839.2022.2096305

ENGELKE, T., S. HÜLSMANN & T. TATLIOGLU. 2002. A comparative study of microsporogenesis and anther wall development in different types of genic and cytoplasmic male sterilities in chives. Plant Breed. 121: 254-258. https://doi.org/10.1046/j.1439-0523.2002.00704.x

ENGELKE, T., D. TEREFE & T. TATLIOGLU. 2003. A PCR-based marker system monitoring CMS-(S), CMS-(T) and (N)-cytoplasm in the onion (Allium cepa L.). Theor. Appl. Genet. 107: 162-167. https://doi.org/10.1007/s00122-003-1230-3

FAHN, A. 1979. Secretory tissues in plants. Academic Press, London.

FAOSTAT. Food and Agriculture Organization of the United Nations [on line]. Available from: http://www.fao.org/faostat. [Accessed: December 2022].

FISHCHUK, O. 2022. Comparative flower morphology in Allium cepa L. (Amaryllidaceae J.St.-Hil.). Notes in Current Biology 1: 36-41. https://doi.org/10.29038/2617-4723-2022-1-1-6

FISHCHUK, O. S. & A. V. ODINTSOVA, 2020. Micromorphology and anatomy of the flowers of Galanthus nivalis and Leucojum vernum (Amaryllidaceae). Regul. Mech. Biosyst. 11: 463-468. https://doi.org/10.15421/022071

FISHCHUK, O. & A. ODINTSOVA, 2021. Micromorphology and anatomy of the flowers in Clivia spp. and Scadoxus multiflorus (Haemantheae, Amaryllidaceae). Acta Agrobotanica 74: 7417. https://doi.org/10.5586/aa.7417

FORD, C. & S. JOHNSON. 2008. Floral traits, pollinators and breeding systems in Syncolostemon (Lamiaceae). Plant Syst. Evol. 275: 257-264. https://doi.org/10.1007/s00606-008-0016-0

FRITSCH, R. M. 1992. Septal nectaries in the genus Allium. In: HANELT, P., K. HAMMER & H. KNUPFFER (eds.), The genus Allium-taxonomic problems and genetic resources, pp. 77-85. Proceedings of an International Symposium, Held at Gatersleben, Germany, June 11-13. 1991. Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben.

FRITSCH, R. M. 2001. Taxonomy of the genus Allium: contribution from IPK Gatersleben. Herbertia 56: 19-50.

GALMARINI, C. R. 2000. Onion cultivars released by La Consulta Experiment Station, INTA, Argentina. HortScience 35: 1360-1362.

GALMARINI, C. R. 2018. Economic and academic importance. In: SHIGYO, M., A. KHAR & M. ABDELRAHMAN (eds.), The Allium Genomes. Compendium of Plant Genomes, pp.1-9. Springer, Cham. https://doi.org/10.1007/978-3-319-95825-5_1

GARCÍA, C. C., M. NEPI & E. PACINI. 2006. Structural aspects and ecophysiology of anther opening in Allium triquetrum. Ann. Bot. 97: 521-527. https://doi.org/10.1093/aob/mcl015

GATICA HERNÁNDEZ, I., F. PALOTTINI, I. MACRI, C. R. GALMARINI & W. M. FARINA. 2019. Appetitive behavior of the honey bee Apis mellifera in response to phenolic compounds naturally found in nectars. J. Exp. Biol. 222: jeb189910. https://doi.org/10.1242/jeb.189910

GAVIOLA, J. 2007. Ficha técnica, producción de semilla de cebolla (Allium cepa L.). INTA-EEA La Consulta, 1-18.

GILLESPIE, S., R. LONG & N. WILLIAMS. 2015. Indirect effects of field management on pollination service and seed set in hybrid onion seed production. J. Econ. Entomol. 108: 2511-2517. https://doi.org/10.1093/jee/tov225

GONZALEZ, A. M. & C. L. CRISTÓBAL. 1997. Anatomía y ontogenia de semillas de Helicteres lhotzkyana (Sterculiaceae). Bonplandia 9: 287-294. https://doi.org/10.30972/bon.93-41497

GURUSHIDZE, M., S. MASHAYEKHI, F. R. BLATTNER, N. FRIESEN & R. M. FRITSCH. 2007. Phylogenetic relationships of wild and cultivated species of Allium section Cepa inferred by nuclear rDNA ITS sequence analysis. Plant Systemat. Evol. 269: 259-269. https://doi.org/10.1007/s00606-007-0596-0

HAVEY, M. J. 2000. Diversity among male-sterility-inducing and male-fertile cytoplasms of onion. Theor. Appl. Genet. 101:778-782. https://doi.org/10.1007/s001220051543

HEEL, W. A. VAN. 1988. On the development of some gynoecia with septal nectaries. Blumea 33: 477-504.

HOLFORD, P., J. CROFT & H. J. NEWBURY. 1991. Structural studies of microsporogenesis in fertile and male-sterile onions (Allium cepa L.) containing the cms-S cytoplasm. Theor. Appl. Genet. 82: 745. https://doi.org/10.1007/BF00227320

HUMAN, H. & S. NICOLSON. 2008. Flower structure and nectar availability in Aloe greatheadii var. davyana: an evaluation of a winter nectar source for honeybees. Int. J. Plant Sci. 169: 263-269. https://doi.org/10.1086/524113

JONES, H. A. & S. L. EMSWELLER. 1936. Development of the flower and macrogametophyte of Allium cepa. Hilgardia 10: 415-428. https://doi.org/10.3733/hilg.v10n11p415

KAMENETSKY, R. & H. D. RABINOWITCH. 2002. Florogenesis. In: RABINOWITCH, H. D. & L. CURRAH (eds.), Allium crop sciences: recent advances, pp 31-57. CABI, Wallingford. https://doi.org/10.1079/9780851995106.0031

KAVITHA, S. J. & P. R. REDDY. 2018. Floral biology and pollination ecology of onion (Allium cepa L.). J. Pharmacogn. Phytochem. 7: 2081-2084.

KEIJZER, C. 1987. The processes of anther dehiscence and pollen dispersal. I. The opening mechanism of longitudinally dehiscing anthers. New Phytol. 105: 487-489. https://doi.org/10.1111/j.1469-8137.1987.tb00886.x

KEIJZER, C. J. 1999. Mechanisms of Angiosperm anther dehiscence, a historical review. In: CLEMENT, C., E. PACINI & J. C. AUDRAN (eds.), Anther and pollen: from biology to biotechnology, pp. 55-68. Springer-Verlag, Berlin Heidelberg. https://doi.org/10.1007/978-3-642-59985-9_6

KIK, C. 2002. Exploitation of wild relatives for the breeding of cultivated Allium species. In: RABINOWITCH, H. D. & L. CURRAH (eds.), Allium crop sciences: recent advances, pp. 81-100. CABI, Wallingford. https://doi.org/10.1079/9780851995106.0081

KUMAR, J. & J. K. GUPTA. 1993. Nectar sugar production and honeybee foraging activity in 3 species of onion (Allium species). Apidologie 24: 391-396. https://doi.org/10.1051/apido:19930405

LÓPEZ, H. & L. GALETTO. 2002. Flower structure and reproductive biology of Bougainvillea stipitata (Nyctaginaceae). Plant biol. 4: 508-514. https://doi.org/10.1055/s-2002-34134

LUQUE, R., H. C. SOUSA & J. E. KRAUS. 1996. Métodos de coloração de Roeser (1972) -modificado- e Kropp (1972) visando a substituição do azul de astra por azul de alcião 8 GS ou 8 GX. Acta Bot. Bras. 10: 199-212. https://doi.org/10.1590/S0102-33061996000200001

MALDONADO, I. B. 2014. Influencia de caracteres florales y factores ambientales sobre la atracción de las abejas y la producción de semilla híbrida de cebolla. Tesis Magister. Universidad Nacional de Cuyo, Argentina. https://orcid.org/0000-0001-6830-2927

MANJUNATHAGOWDA, D. C., MUTHUKUMAR, P., GOPAL, J., PRAKASH, M., BOMMESH, J. C., NAGESH, G. C., ... & ANJANAPPA, M. 2021. Male sterility in onion (Allium cepa L.): origin, evolutionary status, and their prospectus. Genet. Resour. Crop. Evol. 68: 421-439. https://doi.org/10.1007/s10722-020-01077-1

MATSUI, T., K. OMASA & T. HORIE. 1999. Mechanism of anther dehiscence in rice (Oryza sativa L.). Ann. Bot. 84: 501-506. https://doi.org/10.1006/anbo.1999.0943

MONOSMITH, H. R. 1928. Male sterility in Allium cepa. Ph.D. Thesis. University of California. United States.

NEPI, M., P. VON ADERKAS & E. PACINI. 2012. Sugary exudates in plant pollination. In: BALUŠKA, F. & J. VIVANCO (eds.), Secretions and Exudates in Biological Systems, pp. 155-186. Springer-Verlag, Berlin Heidelberg. https://doi.org/10.1007/978-3-642-23047-9_8

ODINTSOVA, A., O. FISHCHUK & A. SULBORSKA. 2013. The gynoecium structure in Dracaena fragrans (L.) Ker Gawl., Sansevieria parva N.E. Brown and S. trifasciata Prain (Asparagaceae) with special emphasis on the structure of the septal nectary. Acta Agrobotanica 66: 55-64. https://doi.org/10.5586/aa.2013.051

PACINI, E., M. NEPI & J. L. VESPRINI. 2003. Nectar biodiversity: a short review. Plant Syst. Evol. 238: 7-21. https://doi.org/10.1007/s00606-002-0277-y

PACINI, E. & M. NEPI. 2007. Nectar production and presentation. In: NICOLSON, S. W., M. NEPI, & E. PACINI (eds.), Nectaries and nectar, pp. 167-214. Springer, Dordrecht.

https://doi.org/10.1007/978-1-4020-5937-7_4

PARKER, F. D. 1982. Efficiency of bees in pollinating onion flowers. Kans. Entomol. Soc. 55: 171-176.

PERNAL, S. & R. CURRIE. 2002. Discrimination and preferences for pollen bassed cues by foraging honeybees, Apis mellifera L. Anim. Behav. 63: 369-390.

https://doi.org/10.1006/anbe.2001.1904

POVEDA, K., I. STEFFAN-DEWENTERA, S. SCHEUB & T. TSCHARNTKE. 2005. Floral trait expression and plant fitness in response to below- and aboveground plant-animal interactions. Perspect. Plant Ecol. Evol. Syst. 7: 77-83. https://doi.org/10.1016/j.ppees.2005.02.002

RUDALL, P. J. 2002. Homologies of inferior ovaries and septal nectaries in monocotyledons. Int. J. Plant Sci. 163: 261-276. https://doi.org/10.1086/338323

RUZIN, S. E. 1999. Plant microtechnique and microscopy. Oxford University Press, Oxford.

SAINI, S. S. & G. N. DAVIS. 1969. Male sterility in Allium cepa and some species hybrids. Econ. Bot. 23:37-49. https://doi.org/10.1007/BF02862970

SCOTT, R. J., M. SPIELMAN & H. G. DICKINSON. 2004. Stamen structure and function. The Plant Cell 16: S46-S60. https://doi.org/10.1105/tpc.017012

SHEMESH-MAYER, E., T. BEN-MICHAEL, N. ROTEM, H. D. RABINOWITCH, A. DORON-FAIGENBOIM, … & R. KAMENETSKY. 2015. Garlic (Allium sativum L.) fertility: transcriptome and proteome analyses provide insight into flower and pollen development. Front. Plant Sci. 6: 271. https://doi.org/10.3389/fpls.2015.00271

SILVA, E. M. & B. DEAN. 2000. Effect of nectar composition and nectar concentration on honey bee (Hymenoptera: Apidae) visitation to hybrid onion flowers. J. Econ. Entomol. 93: 1216-1221. https://doi.org/10.1603/0022-0493-93.4.1216

SILVA, E., B. DEAN & L. HILLER. 2003. Honey bee (Hymenoptera: Apidae) foraging in response to preconditioning with onion flower scent compounds. J. Econ. Entomol. 96: 1510-1513. https://doi.org/10.1093/jee/96.5.1510

SILVA, E. M., B. B. DEAN & L. HILLER. 2004. Patterns of floral nectar production of onion (Allium cepa L.) and the effects of environmental conditions. J. Am. Soc. Hortic. 129: 299-302. https://doi.org/10.21273/JASHS.129.3.0299

SMETS, E. F. & E. M. CRESENS. 1988. Types of floral nectaries and the concepts 'character' and 'character-state'- A reconsideration. Acta Bot. Neerl. 37: 121-128. https://doi.org/10.1111/j.1438-8677.1988.tb01586.x

SMETS, E. F., L. P. RONSE DECRAENE, P. CARIS & P. J. RUDALL. 2000. Floral nectaries in Monocotyledons: distribution and evolution. In: WILSON, K. L. & D. A. MORRISON (eds.), Monocots: Systematics and Evolution, pp. 230-239. CSIRO, Melbourne.

SOTO, V. C., I. J. G. GATICA & C. R. GALMARINI. 2021. Onion (Allium cepa L.) hybrid seed production: Sugar content variation during the flowering period. Crop Sci. 61: 3529-3537. https://doi.org/10.1002/csc2.20597

SOTO, V. C., I. B. MALDONADO, R. A. GIL, I. E. PERALTA, M. F. SILVA & C. R. GALMARINI. 2013. Nectar and flower traits of different onion male sterile lines related to pollination efficiency and seed yield of F1 hybrids. J. Econ. Entomol. 106: 1386-1394. https://doi.org/10.1603/EC13096

SOTO, V. C., I. B. MALDONADO, V. P. JOFRÉ, C. R. GALMARINI & M. F. SILVA. 2015. Direct analysis of nectar and floral volatile organic compounds in hybrid onions by HS-SPME/GC-MS: Relationship with pollination and seed production. Microchem. J. 122: 110-118. https://doi.org/10.1016/j.microc.2015.04.017

SYAMASUNDAR, J. & M. G. PANCHAKSHARAPPA. 1976. A histochemical study of floral nectaries in Dipcadi montanum Dalz. and Allium cepa L. Cytologia 41: 453-457.

https://doi.org/10.1508/cytologia.41.453

STEENHUISEN, S., R. R. AGUSO, A. JÜRGENS & S. JOHNSON. 2010. Variation in scent emission among floral parts and inflorescence developmental stages in beetle-pollinated Protea species (Proteaceae). S. Afr. J. Bot. 76: 779-787. https://doi.org/10.1016/j.sajb.2010.08.008

TCHÓRZEWSKA, D., K. L. DERYŁO & K. WINIARCZYK. 2017. Cytological and biophysical comparative analysis of cell structures at the microsporogenesis stage in sterile and fertile Allium species. Planta 245: 137-150. https://doi.org/10.1007/s00425-016-2597-0

VAN HEEL, W. A. 1988. On the development of some gynoecia with septal nectaries. Blumea 33: 477-504.

VOGEL, S. 1998. Remarkable nectaries: structure, ecology, organophyletic perspectives III. Nectar ducts. Flora 193: 113-131. https://doi.org/10.1016/S0367-2530(17)30827-7

WEBERLING, F. 1992. Morphology of flowers and inflorescences. Cambridge University Press, Cambridge.

WILSON, Z. A., J. SONG, B. TAYLOR & C. YANG. 2011. The final split: the regulation of anther dehiscence. J. Exp. Bot. 62: 1633-1649. https://doi.org/10.1093/jxb/err014

YU, N. & S. KIM. 2021. Identification of Ms2, a novel locus controlling male-fertility restoration of cytoplasmic male-sterility in onion (Allium cepa L.), and development of tightly linked molecular markers. Euphytica 217: 191. https://doi.org/10.1007/s10681-021-02927-4

Publicado

2023-06-01

Número

Sección

Número especial: Amaryllidaceae

Cómo citar

“Estructura Del Nectario Floral, presentación Del néctar Y análisis Morfo-anatómico De Anteras De líneas androfértiles Y androestériles En Cebolla (Allium Cepa, Amaryllidaceae)”. 2023. Boletín De La Sociedad Argentina De Botánica 58 (3). https://doi.org/10.31055/1851.2372.v58.n3.38251.

Artículos similares

1-10 de 53

También puede Iniciar una búsqueda de similitud avanzada para este artículo.