Micropropagation of Anredera cordifolia (Basellaceae)
DOI:
https://doi.org/10.31055/1851.2372.v59.n4.44826Keywords:
Biotecnología., Anredera cordifolia, Aclimatización, Cultivo de tejidos, Propagación in vitroAbstract
Background and aims: The potato vine (Anredera cordifolia) is a climbing plant of the Basellaceae family, with extensive potential for both food and medicinal purposes. The aim of this work was to develop an efficient micropropagation protocol for this species.
M&M: Different factors that affect the different stages of the process were evaluated, including adjustments in the disinfection of plant material for aseptic culture establishment, in vitro multiplication, and greenhouse acclimatization of the regenerated plants.
Results: For aseptic establishment, the most effective treatment in avoiding contamination, allowing explant survival, and promoting plant regeneration was the use of 1.7% active Cl. In the in vitro multiplication stage, the influence of the Murashige and Skoog (MS) basal medium and its dilutions were tested. It was demonstrated that the original concentration of MS was the most suitable for plant regeneration and promoted the highest rates of in vitro multiplication. In vitro plants derived from different dilutions of the MS basal medium were transferred to ex vitro growth conditions. Plants regenerated in MS reduced to a quarter exhibited the highest survival rate compared to the other treatments.
Conclusion: This study constitutes the first attempt to the micropropagation of A. cordifolia. This contribution is crucial for the establishment of an efficient large-scale propagation system as well as for germplasm preservation of this species.
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Copyright (c) 2024 Silvia C Schaller, Natalia R. Dolce, Luis A. Mroginski, Ricardo D. Medina
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