Inducción de respuesta inmune primaria por células dendríticas de rata
DOI:
https://doi.org/10.31053/1853.0605.v57.n2.38493Keywords:
dendritic cells, rats, immunity, toleranceAbstract
In this work we generated dendritic cells (DC) from rat bone marrow cultures stimulated with GM-CSF and IL-4. After 10 days of
culture, we obtained numerous mature DC showing morphological characteristics of DC and high levels of MHC class II molecules.
Also, we isolated DC from rat spleen on the bases of their differential adherence and lowdensity properties. The purity of these cells was > 90 % according lo morphology and MHC class II expression. To evaluate the capacity of bone marrow DC, immature spleen DC or spleen DC cultured 24h with GM-CSF (mature spleen DC), lo elicit an immune response lo ovalbumin (OVA), DC were loaded with this antigen and transferred lo normal rats. Both bone marrow and spleen DC induced delayed type hypersensivity responses (DTH).
However, mature DC from spleen induced a stronger immune response against OVA with the highest DTH values (p < 0,05). These differences in the induction of the immune response correlated with higher expression of MHC class II molecules on mature DC.
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