Structural variations produced IN VITRO by gonadotrophins and steroid hormones on the cell surfaces of the ovarian epithelium of the chick embryo
DOI:
https://doi.org/10.31053/1853.0605.v49.n2.45078Palabras clave:
ovaries, chick embryo, gonadotrophins, Steroids, culturesResumen
The purpose of the present work was to analyze the structural variations producen in vitro" by hormones on thccli surfaces of the ovarian c'pitheliuni of the chick embryo, in relation to the growth of the left ovary and atrophy of the right one. Explants of both ovaries lirom embryos at 7 to 19 days of development were separately cultured for 4 days in MEM with 10 % of fetal bovinc serum (control), and plus 17- /3-estradiol, testosterone propionate, progestcrone, FSH, LH or hCG added indiviclually to cultures (experimental).
The cultures were processecl for thei st.ructural, ultrastructural and cytocheniical study. In control cultures, membrane differentiations and mucosubstan(eS \vere similar in both ovarics and at all ages to those in ovo. 17-/9-estradiol produced a greater development of microvilli, junci ional complexes and mucinc in the epitbelial eclis and interdigitations in the germ celis of the left ovary, while in the right gonad there was ccli regression. Testosterorie ami progesterone evoked in the left gonad a response similar to that obtainecl with Ihe eStrOgen, whereas in the right ovary no changes were observed with respe't t) controis. FSH lcd to cellular regression in both ovaries. Finally. with L or hCG the changes produced in thleft ovary were similar to those induccd by the estrogen, and in the right one an mercase of membrane differentiations and of mucosubstances ielatcd lo Iliem vas found in comparison to conirois. The rcsults obtainecl in expiants of ovarics at 7 days of development and cultured for 4 days, inclicatc that the modifications of the ccli suri aces of the, ovarian epitheliurn of the chick embryo would he related to the action of steroid and gonadotrophic hormones at the start of gonaclal differentiation.
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