Historical divergence in Anadenanthera colubrina var. cebil (Leguminosae) analyzing an intronic region of chloroplast DNA.

Abstract

Historical divergence in Anadenanthera colubrina var. cebil (Leguminosae) analyzingan intronic region of chloroplast DNA. Anadenanthera colubrina var. cebil is a native woody treefrom South America which presents a discontinuous distribution which is similar to that observed forSeasonally Dry Tropical Forests (SDTFs). To evaluate the probable effects of climate changes during Pleistocene in A. colubrina var cebil distribution, the chloroplast trnL intron sequence of 48 individuals from Paranaense and Yungas phytogeographic provinces were analyzed. A SNP in the position 501defined two haplotypes. These haplotypes were fixed according to phytogeographic provinces ofindividual’s origin. The sequences from the trnL intron of these haplotypes were compared with sequences from the same region available in GenBank public database. Three more haplotypes weredefined based on the alignment analyses of these sequences. A network was constructed to determinethe phylogenetic relationships between haplotypes. The estimation of the genetic distance betweenhaplotypes reached values among 0 and 0.017, while the divergence time between haplotypes wasestimated among 0 and 5,600,000 years.