Analyses of morpho-anatomic variables in Tephrocactus (Cactaceae): Correlation with ploidy levels and mappiong in its philogeny
DOI:
https://doi.org/10.31055/1851.2372.v57.n1.34773Keywords:
Cactaceae, mapping morpho-anatomy,, morpho-anatomy, ploidy level,, Tephrocactus.Abstract
Background and aims: Tephrocactus is a genus of Cactaceae belonging to the Opuntioideae subfamily, with most of its species endemic to Argentina. It comprises bushes with lax branches formed by globose joints, which are often fragile and detach generating new plants. Clonal reproduction is associated with the occurrence of high ploidy levels in the genus. The aim of this work was to compare morpho-anatomical traits of the epidermis and underlying tissues of Tephrocactus taxa with different ploidy levels (2n = 22, 44, 77, 88, 242 and 319) and to evaluate them in a phylogenetic context.
M&M: The epidermis and underlying tissues of specimens were studied by means of classical histological techniques. The morpho-anatomical variables were statistically compared and mapped on the phylogeny of the genus.
Results: The stomatal frequency, the frequency of ordinary epidermal cells, the stomatal index, and the stomata length were determined and the underlying tissues (hypodermis) were analyzed with special reference to the shape, frequency and arrangement of druses. All the variables studied showed statistically significant differences among taxa. A positive relationship was found between ploidy levels and size of all types of epidermal cells and druses.
Conclusions: The common ancestor of the group would have small stomata, an intermediate frequency of ordinary epidermal cells, and druses in their hypodermis. The characteristics of the epidermis and underlying tissues correlate with the level of ploidy in the Tephrocactus taxa studied.
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ALMIRÓN, M. G. & E. MARTÍNEZ CARRETERO. 2013. Tephrocactus aoracanthus (Lem.) Lem. Reproducción sexual y clonal en un cactus dominante del desierto hiperárido argentino. J. Prof. Assoc. Cactus Dev. 15: 20-31.
ANDERSON, E. F. 2001. The cactus family. Portland: Timber Press. Estados Unidos.
ARYAVAND, A., B. EHDAIE, B. TRAN & J. G. WAINES. 2003. Stomatal frequency and size differentiate ploidy levels in Aegilops neglecta. Genet. Resour. Crop Evol. 50: 175-182. https://doi.org/10.1023/A:1022941532372
BALDWIN, S. J. & B. C. HUSBAND. 2013. The association between polyploidy and clonal reproduction in diploid and tetraploid Chamerion angustifolium. Molec. Ecol. 22: 1806-1819. https://doi.org/10.1111/mec.12217
BECK, S. L., R. W. DUNLOP & A. FOSSEY. 2003. Stomatal length and frequency as a measure of ploidy level in black wattle, Acacia mearnsii (de Wild). Bot. J. Linn. Soc. 141: 177-181. https://doi.org/10.1046/j.1095-8339.2003.00132.x
BRAVO HOLLIS, H. & L. SCHEINVAR. 1999. El interesante mundo de las cactáceas. Fondo de Cultura Económica, UNAM. México.
CENIZO, V. J., M. B. MAZZOLA, B. M. C. MOLÁS & A. G. KING. 2013. Características morfológicas y anatómicas de las plántulas de Trichocereus candicans (Cactaceae). Bol. Soc. Arg. Bot. 48: 453-464. https://doi.org/10.31055/1851.2372.v48.n3-4.7546
D’AMBROGIO DE ARGÜESO, A. 1986. Manual de Técnicas en Histología Vegetal. Ed. hemisferio sur, Argentina.
DI FULVIO, T. E. 1976. Observaciones en epidermis de Notocactus y Wigginsia (Cactaceae). Kurtziana, 9: 63-80.
DI RIENZO, J. A., F. CASANOVES, M. G. BALZARINI, L. GONZALEZ, M. TABLADA & C. W. ROBLEDO. 2017. InfoStat versión 2017. Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina.
DILCHER, D. L. 1974. Approaches to the identification of angiosperm leaf remains. Bot. Rev. 40: 1-157. https://doi.org/10.1007/BF02860067
FAHN, A. & D. F. CUTLER. 1992. Xerophytes. Encyclopedia of Plant Anatomy–Gebrüder Borntraeger, D-1000 Berlin, Alemania.
FAIGÓN, A., B. G. GALATI, S. ROSENFELDT & R. KIESLING. 2011. Epidermal characters of Pterocactus (Opuntioideae, Cactaceae). Haseltonia 16: 57-66. https://doi.org/10.2985/1070-0048-16.1.57
GIBSON, A. C. & P. S. NOBEL 1986. The cactus primer. Harvard University Press. Estados Unidos. https://doi.org/10.4159/harvard.9780674281714
GUERRA, A. & C. MEDRI. 2015. Diferenciação morfoanatômica de Lepismium cruciforme (Vell.) Miq. (Cactaceae) do dossel e do sub-bosque. SaBios-Rev. Saúde e Biol. 10: 43-51.
GRANT, V. & K. A. GRANT. 1980. Clonal microspecies of hybrid origin in the Opuntia lindheimeri group. Bot. Gaz.141: 101-106. https://doi.org/10.1086/337129
HERNÁNDEZ, M., T. TERRAZAS, A. D. ALVARADO & M. L. CAVAZOS. 2007. Los estomas de Myrtillocactus geometrizans (Mart. Ex. Pfeiff.) console (Cactaceae): variación en su área de distribución. Rev. Fitotecnia Mex. 30: 235-240.
HERNÁNDEZ‐HERNÁNDEZ, T., H. M. HERNÁNDEZ, J. A. DE‐NOVA, R. PUENTE, L. E. EGUIARTE & S. MAGALLÓN. 2011. Phylogenetic relationships and evolution of growth form in Cactaceae (Caryophyllales, Eudicotyledoneae). Amer. J. Bot. 98: 44-61. https://doi.org/10.3732/ajb.1000129
HERRERA-MARTÍNEZ, V., L. RIOS-HERNÁNDEZ, C. GARCIDUEÑAS-PIÑA, A. LARA-IBARRA, A. ADABACHE-ORTIZ, R. E. SORIA-GUERRA, E. PÉREZ-MOLPHE-BALCH & J. F. MORALES-DOMÍNGUEZ. 2015. Effect of culture conditions on stomatal density and stomatal index in four cactus species. Haseltonia 20: 43-51. https://doi.org/10.2985/026.020.0108
HUNT, D., N. TAYLOR & C. GRAHAM. 2006. The new cactus lexicon: descriptions and illustrations of the cactus family. Milborne Port: David Hunt Books.
KALASHNYK, H., N. NUZHYNA & M. GAIDARZHY. 2016. Anatomical and morphological features of seedlings of some Cactoideae Eaton (Cactaceae Juss.) species. Acta Agrobot. 69: 1-11. https://doi.org/10.5586/aa.1697
KIESLING, R. 1975. Los géneros de Cactaceae de Argentina. Bol. Soc. Arg. Bot. 16: 197-227.
KIESLING, R. 1984. Estudios en Cactaceae de Argentina: Maihueniopsis, Tephrocactus y géneros afines (Opuntioideae). Darwiniana 25: 171-215.
LAS PEÑAS, M. L., L. OAKLEY, N. C. MORENO & G. BERNARDELLO. 2017. Taxonomic and cytogenetic studies in Opuntia ser. Armatae (Cactaceae). Botany 95: 101-120. https://doi.org/10.1139/cjb-2016-0048
LAS PEÑAS, M. L., R. KIESLING & G. BERNARDELLO. 2019. Phylogenetic reconstruction of the genus Tephrocactus (Cactaceae) based on molecular, morphological, and cytogenetical data. Taxon 68: 714-730. https://doi.org/10.1002/tax.12092
LOZA-CORNEJO, S. & T. TERRAZAS 2003. Epidermal and hypodermal characteristics in North American Cactoideae (Cactaceae). J. Plant Res. 116: 27-35. https://doi.org/10.1007/s10265-002-0066-2
MADDISON, W. P. & D. R. MADDISON. 2018. Mesquite: a modular system for evolutionary analysis. Version 3.51 http://www.mesquiteproject.org.
MADLUNG, A. 2013. Polyploidy and its effect on evolutionary success: old questions revisited with new tools. Heredity 110: 99-104. https://doi.org/10.1038/hdy.2012.79
MANDUJANO, M., J. GOLUBOV & J. REYES. 2002. Lo que usted siempre quiso saber sobre las cactáceas y nunca se atrevió a preguntar. Biodiversitas 40: 4-7.
MAUSETH, J. D. 2005. Anatomical features, other than wood, in subfamily Opuntioideae (Cactaceae). Haseltonia 2005: 113-125. https://doi.org/10.2985/1070-0048(2005)11[113:AFOTWI]2.0.CO;2
MELARAGNO J. E., B. MEHROTRA & A. W. COLEMAN. 1993. Relationship between endopolyploidy and cell size in epidermal tissue of Arabidopsis. Plant Cell 5: 1661-1668. https://doi.org/10.1105/tpc.5.11.1661
MORRIS, M. W., W. L. STERN & W. S. JUDD. 1996. Vegetative anatomy and systematics of subtribe Dendrobiinae (Orchidaceae). Bot. J. Linn. Soc.120: 89-144. https://doi.org/10.1111/j.1095-8339.1996.tb00483.x
NOBEL, P. S. 2002. Cacti: biology and uses. Berkeley: University of California Press. Estados Unidos.
PRABHAKAR, M. 2004. Structure, nomenclature and classification of stomata. Acta Bot. Sinica 44: 242-252.
PINKAVA, D. J, M. A. BAKER, B. D. PARFITT & M. W. MOHLENBROCK. 1985. Chromosome numbers in some cacti of western North America. Syst. Bot. 10: 471-483. https://doi.org/10.2307/2419140
PINKAVA, D.J. 2002. On the evolution of the continental North American Opuntioideae. Pp. 59–98 in: Hunt, D. & Taylor, N. (eds.), Studies in the Opuntioideae (Cactaceae). Succulent Plant Research 6. Milborne Port: David Hunt Books.
RÊGO, M. D., E. R. RÊGO, C. H. BRUCKNER, F. L. FINGER & W. C. OTONI. 2011. In vitro induction of autotetraploids from diploid yellow passion fruit mediated by colchicine and oryzalin. Plant Cell, Tissue and Organ Culture (PCTOC).107: 451-459. https://doi.org/10.1007/s11240-011-9995-6
RITZ, C. M., J. REIKER, G. CHARLES, P. HOXEY, D. HUNT, M. LOWRY, W. STUPPY & N. TAYLOR. 2012. Molecular phylogeny and character evolution in terete-stemmed Andean opuntias (Cactaceae−Opuntioideae). Molec. Phylog. Evol. 65: 668-681. https://doi.org/10.1016/j.ympev.2012.07.027
SANTEN, E. V. & M. D. CASLER 1986. Evaluation of indirect ploidy indicators in Dactylis L. subspecies. Crop Sci. 26: 848-852. https://doi.org/10.2135/cropsci1986.0011183X002600050002x
SOFFIATTI, P. & V. ANGYALOSSY. 2007. Anatomy of Brazilian Cereeae (subfamily Cactoideae, Cactaceae): Arrojadoa Britton & Rose, Stephanocereus A. Berger and Brasilicereus Backeberg. Acta Bot. Bras. 21: 813-822. https://doi.org/10.1590/S0102-33062007000400006
STACE, C. A. 1965. Cuticular studies as an aid to plant taxonomy. Bull. Br. Publisher: British Museum 4: 62-63.
TAN, G. Y. & G. M. DUNN. 1973. Relationship of stomatal length and frequency and pollen-grain diameter to ploidy level in Bromus inermis Leyss. Crop Sc. 13: 332-334. https://doi.org/10.2135/cropsci1973.0011183X001300030014x
TERRAZAS, T. & S. ARIAS. 2002. Comparative stem anatomy in the subfamily Cactoideae. Bot. Rev. 68: 444-473. https://doi.org/10.1663/0006-8101(2002)068[0444:CSAITS]2.0.CO;2
UPADHAYAYA, M. K. & N. H. FURNESS. 1994. Influence of light intensity and water stress on leaf surface characteristics of Cynoglosum officinale, Centaurea spp. and Tragopogon spp. Canad. J. Bot. 72: 1379-1386. https://doi.org/10.1139/b94-169
VÁZQUEZ-SÁNCHEZ, M., T. TERRAZAS & S. ARIAS. 2016. Comparative morphology and anatomy of Backebergia militaris (Echinocereeae–Cactaceae) cephalium. Plant Syst. Evol. 302: 245-256. https://doi.org/10.1007/s00606-015-1256-4
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