Patología de la placenta de rata inducida por la deficiencia de ácidos grasos
DOI:
https://doi.org/10.31053/1853.0605.v59.n2.38489Palavras-chave:
Placenta de rata, Glándula metrial, Laberinto, ácidos grasos, ácido a-linolénicoResumo
Los lípidos son componentes importantes de las células, tanto desde el punto de vista estructural como funcional. Además de intervenir en funciones de transporte, reconocimiento celular e inmunidad, los Acidos Grasos Esenciales (AGE) son importantes para el mantenimiento funcional y estructural de los organismos animales. En nuestro laboratorio se demostró que ratas preñadas alimentadas con dietas deficientes en ácidos grasos esenciales (DAGE) y otras con las mismas dietas pero suplementadas con 5% de aceite de maíz, (rico en ácido linoleico) mostraron alteraciones enel desarrollo placentario. En el presente trabajo, 57 ratas hembras de una cepa Wistar fueron alimentadas desde el destete con una de las siguientes dietas: 1: DAGE; 2 :DAM (dieta DAGE más aceite de maíz
alta proporción de ácido linoléico); 3: DAS (dieta DAGE más aceite de soja, alta proporción de ácidos linoleico y a-Iinolénico)
y 4 :DC (dieta co-mercial). Después de los 3 meses de edad los animales fueron preñados y se sacrificaron al 13 día de la gestación. Los cuernos uterinosfueron disecados y los sitios de implantación se fijaron en formo] y se incluyeron en parafina. Las observaciones se realizaron sobre cortes coloreados con 1-1/E y se estudió la placenta corioalantoidea el número de implantaciones y el de reabsorciones
.La glándula metrial de ratas DAGE y DAM presentaron modificaciones estructurales en relación a las DC. Los hallazgos más notables fueron : Indiferenciación de las células granulares metriales y aumento de la cantidad de tejido conectivo. En las ratas DAS ,en cambio,el aspecto de la glándulametrial era similar a lo observado en el grupo DC. En los grupos DAGE y DAM el laberinto estaba ensanchado, con septos vasculares pero en el grupo DAS eran similares al DC, delgados y vasculares. No se detectaron diferencias en el número de implantaciones y reabsorciones. Los presentes resultados sugieren que el ácido a-linolénico dietético es esencial para que la placenta de rata alcance su desarrollo normal.
Downloads
Referências
Aes-Jorgensen. Essential Fatty Acids Physiol Rew. 41 :1-51. 1961.
Bouve. J.M ; Durand, G; Pascal, G; and You-You, A. Brain Ccli and Tissue. Recovery in Rats Made deficient in n-3 Fatty Acids by alteration of Dietary. Fat. J. Nutr. Jan ; 119 (1) :15-22. 1989.
Eynard, A. R.; Monis, B; Kalinec, F.; Leguizamón, R; Increased proliferation of the epitehelium of the proximal alimentary tract of EFA-deficient rats. A light and electron microscopy study. Exp. Mol Pathol 1982: 36: 135-143.
Araya , J; Aguilera, A.M.; Soto, A; Masson, L; Composición lipídica de la placenta de ratas con restricción de proteínas y deficiencias de acidos grasos esenciales Arch. Lat. Amer. Neet: 36:3271337 .1986.
Menon, N.K.; C. Moore y G. A Dhopeshwenkar. Effect of essential fatty deficiency no material placental and fetal tissues . J. Nutr., 111: 1602-1610, 1981.
Diamant Y.Z. y E. Shafir. Erizimes of carbohydrate and lipids metabolism in the placenta and liver of pregnant rats. Biochimice et Biophysice Acta 279; 424-439. 1972.
Burr y Burr. A new deficiency disease produced by rigid exclusion of fat from the diet . J. Biol. Chem. 1929.
Maeder .The effect of fat in simplified diets on the reproductive organs of the female albino ratas during gestation. Anat. Rec. 70:405-410.1983.
Boissouneault, G.A., Johston. PV. Essential Fatty acid prostaglandin synthesis and hu- moral immunity in Lewis rats. J. Nutr. 70:405-410. 1983.
Gordon, D.; Bray, MA.; and J. Morley. Control of limphokine secretion by Prostaglandin. Nature (London ), 262:401- 402 ; 1976.
Bourne, H.R.; Linchtenstein ,L.M; Melmon, K.L.; Henney, C.S.; Weinstein, Y. and G.H. Shearer. Modulation of inflammation and immunity by cyclic AMP: Receptors of inflammation regulate many leucocyte functions. Science, 184: 19-28. 1974.
Cabral, NRA; Novak, I.T.C. Effects of exogenous gangliosides "in vitro" on Cytologic and Immunologic activities, of Lymphocytes from patients with Chagas Disease (abstract) Mcm. Inst. Oswaldo Cruz, Rio de Janeiro, Vol.91, Suppl., November, P251. 1996.
Bray, M. A. The pharmacology and pathophysiology of leucotriene B4 . Br. Mcd. BulI., 39; 249-254.1983.
Resnik, R. and G.W. Brink. Uterine vascular response to prostaglandin in nonprcgnant sheep. Am. J. Obstet. Gynecol., 137 :267- 270; 1980.
Scharmamm, W.; Einer-Jensen, N.Brown, M.B. and J.A. Mc Craken Effect of four myometrium. Biol. Reprod. 30: 523-231. 1984
Moncada, S. ; Gryglewski, R.; Bunting, S. And J.R. Vane: An enzyme islated from arteries transforms prostaglandin endoperoxides to an unstable substance that inhibits platelet aggregation. Nature (London ), 263(5579): 663-665.1976.
Parlanti, I.A.; Orellana L.C. The influence of essential fatty acid deficient diet on the reproductive performance of female rats. Reprod. Nutr. Develop. 25: 851-860 1985.
Weine and Sprecher H. Arachidonic acid 5, 8, 11-eicosatrienoic acid 5,8, 11, 14,17- eicosapentaenoic acid. Dietary manipulation of these acids in rat liver and platelet phospho-lipids and their incorporation into human platelet lipids Biochem Biophs Acta: 792:293-303.1984.
Bourne, H.R., Linchtenstein, L.M. ; Melnon, K.L.; Hennz, C.S.; Weinstein and G.M. Shearer. Modulation on inflammation and immunity by cyclic AMP: Receptors for vasoactive hormones and mediators of inflammation regulate many leucocyte functions. Science, 184 :19-28 .1974.
Hunt, CE.; Gora, P.; Inwod, R.J.; Pulmonary effects of Intralipid. The role of intralipid as prostagaldin precursor. Prog. Lipid. Res; 20:199-04; 1981.
Dallenbach, H. ; Battista, J,V.; Dallenbach; F.D.; Irimunological and histochernical localization of relaxin in the metrial gland of pregnant rat. Am.J.Anat. 117:433,450. 1965.
Swabe, C.; Steinetz, B.; Weiss, G.; Segaloff, A.; Mc Donaid, J. K.; O Byrne, J.; Hochman, J. ; Carriere, B. and Glodsmith, I. Relaxin. Recent Prog. Horn. Res., 34:123-221. 1978.23
Stewar, 1; Peel, S. An investigation into the differentiation of granulated metrial gland celis in the early pregnant mouse uterus. J.Anat., 137: 85-93.1978.
La] a, P. K.; Kearms, M.; and Colavincenzo. Cells of the fetomaternal interface: Their role in the maintenance of viviparous pregnant uterus
. Am.J. Anat. 170 :501- 510. 1984.
Ye, W. ZhengL.M.; Young ,J.D.;Liu,C.C, The involment of interleukin (IL) 15 in regulating the differentiation of granulated metrial gland cells in mouse pregnant uterus. J. Exp. Med., Dec. 184: 6 2405-10 ;!996.
Kiso, Y.; Kusakabe, K.; Tokunaga, Y; Makita, M.; Okada, T.; Sasaki, F.; A study of granulated metrial gland celis in the pregnant, alyrnphoplasia (aly/aly) mice . J. Vet. Med. Sci, Dec. 59: 12, 1137-41:1977.
Croy, A. B.; Daniel S. and Guimond M., Departarnent of Biomedical Sciences, University of Guelp, on Cnada NIG 2W!.Influences of the Maternal immune system of fetal Development . International Workshop for the Maternal -place ntal-fetal dialogue . Maui Marriot, Hawaii . USA 1996.
Parr, E., Young , L.H.; Parr, M.B.; Young, J.D. Granulated metrial gland celis of
pregnant mouse uterus are natural Killer like celis that contain perforin and serme esterases. J. Inmunol. Oct. 1; 145 (7):2365- 72.1990.
Bulner, D. Further studies on the granulated metrial gland celis of the pregnant rat.J.Anat, 103: 479-489. 1968.
Phillips, C.A, ; Poyser, N.I. Studies on the involment of prostaglandin in implantation in the rat . J. Reprod. Fertil., 62: 73-81. 1981.
Siiteri, P.K.; and Stites, D.P. Inmunologic and endocrine interrelationships in pregnancy. Biol. Reprod. 26: 1-14. 1982.
Kennedy, T.C.; Lukash, L.A. Induction of decidualization in ratas by the intrauterine infusion of prostaglandins. Biol. Reprod.27: 253-260. 1982.
Hiller, K.; Wailis, R. Collageri solubility and tensile properties of the rat uterine cervix in late pregnancy effects of arachidonic and prostaglandin F2 J. Endocrino] 95: 341-347. 1982.
Norstrón, A.L.; Wilhelmason; Hamberger, L.; Experimental studies on the influence of prostaglandin on the connective Tissue of the 1-Turnan Cervi Uteri.Acta Obstet Gynecol Scand (suppl) 113: 167-170; 1970.
Kennedy, T.G. Timing of uterine sensitivity for the decidual celi reaction Role of prostaglandins. Biol-Reprod 30-544- 555.1980.
Crawford, M; Background to essential fatty acids and their prostanoid derivates. Br. Med.Bull. 39: 210-213. 1983.
Agradi, E.; Tremoli, E.; Petroni, A.; and Galli, C.; Dietary linoleic acid in Tissue Lipids and Production of TXB2 and PG 12 in the Cardiovascular System. Prog. Lipid. Res. 20: 485-489, 198!.
Friedmasn, Z.; Effects of dietary variation in linoleic acid content on prostaglandinSynthesis in infants. Prog. Lipid. Res. 20: 59-66. 1981.
Presler, L; Whitten, CF.; Paulsreid andHolman, R. T. Serum fatty acid changes during fat free intravenous therapy. J. Pediatr. 78: 1067-1072.1971.
Hiller, K.; Wailis, R.; Collagen solubility and tensile properties of the rat uterine cervix in late pregnance effects of arachidonic acid and prostaglandin P2. J. Endocrino]., 95: 341-347. 1982.
Norstrón, A.l.; Wilhe!mason; Hamberger, L.; Experimental studies on the influence of prostaglandins on the connective tissue of the human cervix uteri. Acta Obstet.Gynecol Scand (suppl) 113:167-170. 1983.
Downloads
Publicado
Edição
Seção
Licença
Copyright (c) 2022 Universidad Nacional de Córdoba
Este trabalho está licenciado sob uma licença Creative Commons Attribution-NonCommercial 4.0 International License.
A geração de trabalhos derivados é permitida, desde que não seja feita para fins comerciais. O trabalho original não pode ser usado para fins comerciais.
Como Citar
