Using X-ray microtomography imaging for archaeological wood charcoal studies
DOI:
https://doi.org/10.31048/py9r4w88Keywords:
Microtomography images, Anthracological analysis, Experimental charred wood samples, 3D Imaging, Virtual modelsAbstract
There are currently hardly any references to anthracological analyses using microtomography. Expectations about its potential, suggested in part by its use on similar materials, need to be evaluated in anthracological practice, according to the specificity of its subject. This paper seeks to highlight some benefits and disadvantages of the technique in the analysis of archaeological charcoal assemblages and to point out aspects to which it offers significant contributions. Here we present the steps of an experimental plan for microtomographies acquisition applied to a set of modern reference samples of wood charcoal. A brief protocol on how to prepare contemporary charcoal samples for microtomographic imaging is also included in the proposal. Digital analysis procedures for a number of diagnostic anatomical characters used for the taxonomic identification of woods and charcoals are described below. Lastly, descriptions of the microscopic anatomy of some specimens are presented, based entirely on the analysis of virtual models obtained by microtomography. The results suggest that X-ray microtomography provides quality data for anthracology. However, time and resources needed to study large sample sets, which are the privileged basis of this disciplinary field, could interfere with its applicability. On the other hand, microtomography offers several advantages for studies based on small and/or taxonomically homogeneous assemblages of charcoals, or even specific samples. These are related to its capabilities in exploring the whole object through unlimited visualization planes, the automation of measurements and the bulk of morphological data that can be extracted from three-dimensional images, whether for index calculations or other statistical markers, the non-destructiveness involved in sample preparation and analytical processes, the preservation of physical specimens and the simultaneous documentation and storage of large amounts of available information as virtual models of the studied materials.
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References
Andonova, M. (2021). Ancient basketry on the inside: X-ray computed microtomography for the non-destructive assessment of small archaeological monocotyledonous fragments: examples from Southeast Europe. Heritage Science, 9(1), 158. https://doi.org/10.1186/s40494-021-00631-z
Azeredo, S. R., Cesareo, R., Jordan, R. F., Fernandez, A., Gigante, G. E., Bustamante, A., y Lopes, R. T. (2019). Analysis of precious metals from the tomb of the “Lady of Cao” by X-ray microtomography and digital radiography. X-Ray Spectrometry, 48(5), 499–504. https://doi.org/10.1002/xrs.3013
Baldin, T., Siegloch, A. M., y Marchiori, J. N. C. (2016). COMPARED ANATOMY OF SPECIES OF Calycophyllum DC. (Rubiaceae). Revista Árvore, 40, 759–768. https://doi.org/10.1590/0100-67622016000400020
Barron, A., y Denham, T. (2018). A microCT protocol for the visualisation and identification of domesticated plant remains within pottery sherds. Journal of Archaeological Science: Reports, 21, 350–358. https://doi.org/10.1016/j.jasrep.2018.07.024
Barron, A., Pritchard, J., y Denham, T. (2022). Identifying archaeological parenchyma in three dimensions: Diagnostic assessment of five important food plant species in the Indo-Pacific region. Archaeology in Oceania, 57(3), 189–213. https://doi.org/10.1002/arco.5276
Barron, A. (2024). Applications of Microct Imaging to Archaeobotanical Research. Journal of Archaeological Method and Theory, 31(2), 557–592. https://doi.org/10.1007/s10816-023-09610-z
Baruchel, J., Cloetens, P., Härtwig, J., Ludwig, W., Mancini, L., Pernot, P., y Schlenker, M. (2000). Phase imaging using highly coherent X-rays: Radiography, tomography, diffraction topography. Journal of Synchrotron Radiation, 7(3), 196–201. https://doi.org/10.1107/S0909049500002995
Beck, L., Cuif, J.-P., Pichon, L., Vaubaillon, S., Dambricourt Malassé, A., y Abel, R. L. (2012). Checking collagen preservation in archaeological bone by non-destructive studies (Micro-CT and IBA). Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 273, 203–207. https://doi.org/10.1016/j.nimb.2011.07.076
Bello, S. M., De Groote, I., y Delbarre, G. (2013). Application of 3-dimensional microscopy and micro-CT scanning to the analysis of Magdalenian portable art on bone and antler. Journal of Archaeological Science, 40(5), 2464–2476. https://doi.org/10.1016/j.jas.2012.12.016
Bernardini, F., Leghissa, E., Prokop, D., Velušček, A., De Min, A., Dreossi, D., Donato, S., Tuniz, C., Princivalle, F., y Montagnari Kokelj, M. (2019). X-ray computed microtomography of Late Copper Age decorated bowls with cross-shaped foots from central Slovenia and the Trieste Karst (North-Eastern Italy): Technology and paste characterisation. Archaeological and Anthropological Sciences, 11(9), 4711–4728. https://doi.org/10.1007/s12520-019-00811-w
Bird, M. I., Ascough, P. L., Young, I. M., Wood, C. V., y Scott, A. C. (2008). X-ray microtomographic imaging of charcoal. Journal of Archaeological Science, 35(10), 2698–2706. https://doi.org/10.1016/j.jas.2008.04.018
Bodin, S. C., Scheel-Ybert, R., Beauchêne, J., Molino, J.-F., y Bremond, L. (2019). CharKey: An electronic identification key for wood charcoals of French Guiana. IAWA Journal, 40(1), 75-S20. https://doi.org/10.1163/22941932-40190227
Boschin, F., Zanolli, C., Bernardini, F., Princivalle, F., y Tuniz, C. (2015). A Look from the Inside: MicroCT Analysis of Burned Bones. Ethnobiology Letters, 6(2), 258–266. https://doi.org/10.14237/ebl.6.2.2015.365
Brodersen, C. R. (2013). Visualizing wood anatomy in three dimensions with high-resolution X-ray micro-tomography (μCT) – a review –. IAWA Journal, 34(4), 408–424. https://doi.org/10.1163/22941932-00000033
Calo, C. M., Rizzutto, M. A., Carmello-Guerreiro, S. M., Dias, C. S. B., Watling, J., Shock, M. P., Zimpel, C. A., Furquim, L. P., Pugliese, F., y Neves, E. G. (2020). A correlation analysis of Light Microscopy and X-ray MicroCT imaging methods applied to archaeological plant remains’ morphological attributes visualization. Scientific Reports, 10(1), 15105. https://doi.org/10.1038/s41598-020-71726-z
Calo, C. M., Rizzutto, M. A., Watling, J., Furquim, L., Shock, M. P., Andrello, A. C., Appoloni, C. R., Freitas, F. O., Kistler, L., Zimpel, C. A., Hermenegildo, T., Neves, E. G., y Pugliese, F. A. (2019). Study of plant remains from a fluvial shellmound (Monte Castelo, RO, Brazil) using the X-ray MicroCT imaging technique. Journal of Archaeological Science: Reports, 26, 101902. https://doi.org/10.1016/j.jasrep.2019.101902
Chabal, L. (1988). Pourquoi et comment prélever les charbons de bois pour la période antique: Les méthodes utilisées sur le site de Lattes (Hérault). Lattara, 1, 187–222.
Coubray, S., Zech-Matterne, V., y Mazurier, A. (2010). The earliest remains of a Citrus fruit from a western Mediterranean archaeological context? A microtomographic-based re-assessment. Comptes Rendus Palevol, 9(6–7), 277–282. https://doi.org/10.1016/j.crpv.2010.07.003
Detienne, P., y Jacquet, P. (1983). Atlas d’identification des bois de l’Amazonie et des régions voisines /. Centre Technique Forestier Tropical.
Dierickx, S., Genbrugge, S., Beeckman, H., Hubau, W., Kibleur, P., y Van den Bulcke, J. (2024). Non-destructive wood identification using X-ray µCT scanning: Which resolution do we need? Plant Methods, 20(1), 98. https://doi.org/10.1186/s13007-024-01216-0
Dreossi, D., Favretto, S., Fioravanti, M., Mancini, L., Rigon, L., Sodini, N., Tromba, G., y Zanini, F. (2010). Synchrotron radiation Micro-tomography: A non-invasive tool for th characterization of archaeological wood. In L. Uzielli (Ed.), Wood Science for Conservation of Cultural Heritage. Firenze University Press.
Gálvez, G. I. E. C., Rocha, M. P. da, Klitzke, R. J., y Mora, H. E. G. (2020). Caracterización anatómica y variabilidad de los componentes de la madera de Calycophyllum spruceanum (Benth). Hook. Revista Ciência da Madeira (Brazilian Journal of Wood Science), 11(2), Article 2. https://periodicos.ufpel.edu.br/index.php/cienciadamadeira/article/view/17300
George, M. J., Paris, E. H., Liu, W., López Bravo, R., y Lalo Jacinto, G. (2024). Applications of Micro-CT Imaging in Age-At-Death Estimates of Maya Dogs. Environmental Archaeology, 0(0), 1–16. https://doi.org/10.1080/14614103.2024.2380117
Göldner, D., Karakostis, F. A., y Falcucci, A. (2022). Practical and technical aspects for the 3D scanning of lithic artefacts using micro-computed tomography techniques and laser light scanners for subsequent geometric morphometric analysis. Introducing the StyroStone protocol. PLOS ONE, 17(4), e0267163. https://doi.org/10.1371/journal.pone.0267163
Gonçalves, T. a. P., y Scheel-Ybert, R. (2016). Charcoal anatomy of Brazilian species. I. Anacardiaceae. Anais Da Academia Brasileira de Ciências, 88, 1711–1725. https://doi.org/10.1590/0001-3765201620150433
Gonçalves, T. A. P., y Scheel-Ybert, R. (2017). Primeiro atlas antracológico de espécies brasileiras. Série Livros Digital, 10. http://pantheon.ufrj.br/handle/11422/15320
Grabner, M., Salaberger, D., y Okochi, T. (2009). The need of high resolution μ-X-ray CT in dendrochronology and in wood identification. 2009 Proceedings of 6th International Symposium on Image and Signal Processing and Analysis, 349–352. https://doi.org/10.1109/ISPA.2009.5297695
Haneca, K., Deforce, K., Boone, M. N., Van Loo, D., Dierick, M., Van Acker, J., y Van Den Bulcke, J. (2012). X-Ray Sub-Micron Tomography as a Tool for the Study of Archaeological Wood Preserved Through the Corrosion of Metal Objects. Archaeometry, 54(5), 893–905. https://doi.org/10.1111/j.1475-4754.2011.00640.x
Hernández, W. J. L. (2020). Anatomía de Maderas de 130 Especies de Venezuela. Revista Pittieria, 0, Article 0.
Hubau, W., Bulcke, J. V. den, Kitin, P., Brabant, L., Acker, J. V., y Beeckman, H. (2013). Complementary Imaging Techniques for Charcoal Examination and Identification. IAWA Journal, 34(2), 147–168. https://doi.org/10.1163/22941932-00000013
Kahl, W.-A., y Ramminger, B. (2012). Non-destructive fabric analysis of prehistoric pottery using high-resolution X-ray microtomography: A pilot study on the late Mesolithic to Neolithic site Hamburg-Boberg. Journal of Archaeological Science, 39(7), 2206–2219. https://doi.org/10.1016/j.jas.2012.02.029
Karjalainen, V.-P., Finnilä, M. A. J., Salmon, P. L., y Lipkin, S. (2023). Micro-computed tomography imaging and segmentation of the archaeological textiles from Valmarinniemi. Journal of Archaeological Science, 160, 105871. https://doi.org/10.1016/j.jas.2023.105871
Marconetto, M. B. (2010). Paleoenvironment and anthracology: Determination of variations in humidity based on anatomical characters in archealogical plant charcoal (Ambato Valley, Catamarca, Argentina). Journal of Archaeological Science, 37(6), 1186–1191. https://doi.org/10.1016/j.jas.2009.12.016
Machado, A. S., Silva, A. S. S., Campos, G. N., Gomes, C. S., Oliveira, D. F., y Lopes, R. T. (2019). Analysis of metallic archaeological artifacts by x-ray computed microtomography technique. Applied Radiation and Isotopes, 151, 274–279. https://doi.org/10.1016/j.apradiso.2019.06.016
Mizuno, S., Torizu, R., y Sugiyama, J. (2010). Wood identification of a wooden mask using synchrotron X-ray microtomography. Journal of Archaeological Science, 37(11), 2842–2845. https://doi.org/10.1016/j.jas.2010.06.022
Murphy, C., y Fuller, D. Q. (2017). Seed coat thinning during horsegram (Macrotyloma uniflorum) domestication documented through synchrotron tomography of archaeological seeds. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-05244-w
Nava, A., Coppa, A., Coppola, D., Mancini, L., Dreossi, D., Zanini, F., Bernardini, F., Tuniz, C., y Bondioli, L. (2017). Virtual histological assessment of the prenatal life history and age at death of the Upper Paleolithic fetus from Ostuni (Italy). Scientific Reports, 7(1), 9427. https://doi.org/10.1038/s41598-017-09773-2
Ngan-Tillard, D., Dijkstra, J., Verwaal, W., Mulder, A., Huisman, H. (D J.), y Müller, A. (2015). Under Pressure: A Laboratory Investigation into the Effects of Mechanical Loading on Charred Organic Matter in Archaeological Sites. Conservation and Management of Archaeological Sites, 17(2), 122–142. https://doi.org/10.1080/13505033.2015.1124179
Obata, H., Miyaura, M., y Nakano, K. (2020). Jomon pottery and maize weevils, Sitophilus zeamais, in Japan. Journal of Archaeological Science: Reports, 34(Part A), 102599. https://doi.org/10.1016/j.jasrep.2020.102599
Pritchard, J., Lewis, T., Beeching, L., y Denham, T. (2019). An assessment of microCT technology for the investigation of charred archaeological parenchyma from house sites at Kuk Swamp, Papua New Guinea. Archaeological and Anthropological Sciences, 11(5), 1927–1938. https://doi.org/10.1007/s12520-018-0648-0
Puhar, E. G., Korat, L., Erič, M., Jaklič, A., y Solina, F. (2022). Microtomographic Analysis of a Palaeolithic Wooden Point from the Ljubljanica River. Sensors, 22(6), Article 6. https://doi.org/10.3390/s22062369
Rueden, C. T., Schindelin, J., Hiner, M. C., DeZonia, B. E., Walter, A. E., Arena, E. T., y Eliceiri, K. W. (2017). ImageJ2: ImageJ for the next generation of scientific image data. BMC Bioinformatics, 18(1), 529. https://doi.org/10.1186/s12859-017-1934-z
Scheel-Ybert, R. (2004). Teoria e método em Antracologia. 2- Técnicas de campo e laboratório. Arquivos Do Museu Nacional, 62(4), 343–356.
Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., Tinevez, J.-Y., White, D. J., Hartenstein, V., Eliceiri, K., Tomancak, P., y Cardona, A. (2012). Fiji: An open-source platform for biological-image analysis. Nature Methods, 9(7), 676–682. https://doi.org/10.1038/nmeth.2019
Schneider, C. A., Rasband, W. S., y Eliceiri, K. W. (2012). NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9, 671–675. https://doi.org/10.1038/nmeth.2089
Souza-Pinto, N. R. D., y Scheel-Ybert, R. (2021). Charcoal anatomy of Brazilian species. II. 15 native species occurring in Atlantic or Amazon rainforest. Anais Da Academia Brasileira de Ciências, 93(4), e20190983. https://doi.org/10.1590/0001-3765202120190983
Stelzner, I., Stelzner, J., Gwerder, D., Martinez-Garcia, J., y Schuetz, P. (2023). Imaging and Assessment of the Microstructure of Conserved Archaeological Pine. Forests, 14(2), 211. https://doi.org/10.3390/f14020211
Stelzner, J., y Million, S. (2015). X-ray Computed Tomography for the anatomical and dendrochronological analysis of archaeological wood. Journal of Archaeological Science, 55, 188–196. https://doi.org/10.1016/j.jas.2014.12.015
Stock, S. R. (2008). Microcomputed tomography: Methodology and applications (1st ed.). CRC Press.
Trtik, P., Dual, J., Keunecke, D., Mannes, D., Niemz, P., Stähli, P., Kaestner, A., Groso, A., y Stampanoni, M. (2007). 3D imaging of microstructure of spruce wood. Journal of Structural Biology, 159(1), 46–55. https://doi.org/10.1016/j.jsb.2007.02.003
Van den Bulcke, J., Boone, M., Van Acker, J., Stevens, M., y Van Hoorebeke, L. (2009). X-ray tomography as a tool for detailed anatomical analysis. Annals of Forest Science, 66(5), 508–508. https://doi.org/10.1051/forest/2009033
Villagran, X. S., Strauss, A., Alves, M., y Oliveira, R. E. (2019). Virtual micromorphology: The application of micro-CT scanning for the identification of termite mounds in archaeological sediments. Journal of Archaeological Science: Reports, 24, 785–795. https://doi.org/10.1016/j.jasrep.2019.02.035
Ward, I., Key, M. M., O’Leary, M. J., Carson, A., Shaw, J., y Maksimenko, A. (2019). Synchrotron X-ray tomographic imaging of embedded fossil invertebrates in Aboriginal stone artefacts from Western Australia: Implications for sourcing, distribution and chronostratigraphy. Journal of Archaeological Science: Reports, 26, 101840. https://doi.org/10.1016/j.jasrep.2019.05.005
Wheeler, E. A. (2011). InsideWood—A web resource for hardwood anatomy. IAWA Journal, 32(2), Article 2. https://doi.org/10.1163/22941932-90000051
Wheeler, E. A., Baas, P., y Gasson, P. (Eds.). (1989). IAWA list of microscopic features for hardwood identification. IAWA Bulletin n. s., 10(3), 219–332.
Wheeler, E. A., Gasson, P. E., y Baas, P. (2020). Using the InsideWood web site: Potentials and pitfalls. IAWA Journal, 41(4), 412–462. https://doi.org/10.1163/22941932-bja10032
Whitau, R., Dilkes-Hall, I. E., Dotte-Sarout, E., Langley, M. C., Balme, J., y O’Connor, S. (2016). X-ray computed microtomography and the identification of wood taxa selected for archaeological artefact manufacture: Rare examples from Australian contexts. Journal of Archaeological Science: Reports, 6, 536–546. https://doi.org/10.1016/j.jasrep.2016.03.021
Zhao, G., Qiu, Z., Shen, J., Deng, Z., Gong, J., y Liu, D. (2018). Internal Structural Imaging of Cultural Wooden Relics Based on Three-Dimensional Computed Tomography. BioResources, 13(1), 1548–1562. https://doi.org/10.15376/biores.13.1.1548-1562
Zong, Y., Yao, S., Crawford, G. W., Fang, H., Lang, J., Fan, J., Sun, Z., Liu, Y., Zhang, J., Duan, X., Zhou, G., Xiao, T., Luan, F., Wang, Q., Chen, X., y Jiang, H. (2017). Selection for Oil Content During Soybean Domestication Revealed by X-Ray Tomography of Ancient Beans. Scientific Reports, 7(1). https://doi.org/10.1038/srep43595
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