Influence of diets varying in lipid and protein content on the histopathologic variety of murine salivary gland tumors induced by 9, 10 - dimethyl - 1, 2 - benzanthraceno (DMBA)
Keywords:
salivary glands, tumors, mice, dietary lipids, soy protein, histopathologyAbstract
The purpose of this study was to analyze the influence of diets varying in lipids and proteins on the histopathologic variety of murine salivaiy tumors induced by DMBA. 117 BALB/c mice were assigned to experiments one (El: lipids, males) and two (E2: proteins, males and fima1es). El comprising Soy oil (SO): Corn oil (CO. control); Fish oil (FO) and 01cm (0) groups and E2, soy protein (SP) and casein (C) groups. Tumors were induced by DMBA and the animaJs were sacrificed t week 13' post-induction. Tumor volume was calculated. Tumor sections were stained with HE for histopathologic evaluation. No significant association was found between tumor volume and dietary condition (p> 0. 05). In El, FO animais developed mainly carcinomas (C) (58,8%), the sarcomas (S) and carcinosarcomas (CS) being especially of highgrade type (tumors <600 mm3). In E2, SP animais developed mainly C (55.61/1 o). Although no significantly different (p>0.05), 5 and C were more frequent in female and maTe mice, respectively. In both El aral E2, the biggest tumors (>600 mm3) were mainly high-grade 5 (87.5%-80%). Dietary fat and soy protein appear to influence the tumor histopathology and thus its prognosis.
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