Significativa prolongación del tiempo de conservación de la córnea manteniendo sus tensiones y curva natural es
DOI:
https://doi.org/10.31053/1853.0605.v59.n2.38487Keywords:
Cornea conservations, media, time tensions, curvaturetrame sistem transplantAbstract
The success of cornea transplantationshas always been a concern of world researchers to identify risk factors from the proper surgical
techniques up to the severa] tissue storage procedures, such as the ones used in the new laboratories called eyes banks. However, the integral research works are still limited at present and they continue putting emphasis in the donor cornea storage that guarantee up tofor this tissue feasibility. This research had the aim to contributeto the improvement of the corneal tissue quality taking into account the maintenance of its na- tural curvature and tensions combining themwith sorne standard preservation solutions. This was achieved by rneans of a new mechanical system. Assays with rabbits were designed according to a sequential experimental groups design. In these ones, the study of the feasibility parameters behavior under different conservation media, related to the time, was splitted into four blocks. The first and second ones attended the cornea conservation in the short and intermediate-terms culture mediums as the ones at 40
C degree isolated cornea preservation and the usual ones such as the Me Carey Kaufman, Optisol and Likorol solutions used in the eyes banks. The third nne was designed for the surgical assays comino from the two named groups and the last one focused the time study of these parameters for a new conservation technique, developed as an alternative medium to keep the cornea tissue curvatures and tensions as well as its nutrition and hydration.
The results showed that tissue natural tensions maintenance is an significant factor in the conservation process, which involve the cornea feasibility for the its use in future transplantations. Since the new developed technique is very simple, this study is considered to be a significant contribution for the stoiage and biological material handling steps.
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