Comportamiento ultraestructural de la inervación de las células intersticiales durante la diferenciación del ovario del embrión de pollo cultivado con 17- 8- ESTRADIOL
DOI:
https://doi.org/10.31053/1853.0605.v58.n1.37017Keywords:
ultrastructure, interstitial cells, innervation, chick embryo ovary, in vitro, estradiolAbstract
In a previous work we demonstrated the relationship between nerve fibers and nerve endings and interstitial cells (estrogen producing cells) from the atrophic right ovary and the medulla in the left functioning ovary during embryogenesis in the chick, ¡u ovo. Besides, the local production of neurotrophins by steroidogenic cells is probably involved in the control of ovarian innervation. The objective of the present study was to analyze ultrastructurally the innervation during the diffcrentiation of chick ovary cultured with 17- 13 -estradiol. Explants of right and teft ovaries from seven to nineteen days in ovo development were cultured separately for 4 days in MEM (controls) or in the presence of 17- II -estradiol (problems).
In controls the electron microscopic examination of the innervation explants from chick cmbryo ovaries revealed that the interstitial cells are well innervated. Nerve fibres and nervecndings were observed in close contact with steroid-producing cells, a similar pattern of innervation that those of the fifteen days ovaries inovo det'elopinent. Problems cultured from seven days showed nerve fibres and nerve endings at difference to controls. These results ¡o vitro suggest that innervation of the ovaries is controled by indirect mechanism via the hypothalamic-pituitary system and local production factors. More experiments are necessary to confirm this results.
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