Aplicación de la técnica de fluorescencia directa en el diagnóstico de las micosis superficiales
DOI:
https://doi.org/10.31053/1853.0605.v59.n1.36960Keywords:
superficial mycosis, dermatofitos, calcofluor white Technique, direct fluorescence br fungiAbstract
A Direct Microscopic Exam (DME)- in 40x with KOH at 40% and heat- applied on materials obtained by sciapingfrom injuries on patienis infected with superficial mycosis, results effective in the detection and identification of infectious organisms based on
iheir morphological features. This exam requires the ability of an experienced medical professional doe to the fact thai the mycouc clement are not typical or are found in very small amounts. In this study we applied the calcofluor white and lluorescence iechnique togeiher with the DME with KOH at 401,11. and heat. In order lo do it, 100 sarnples obiained by scraping from skin of sniall and big folds and nails from feeis and hands, sere analyzed. Wc found a lower perceniage of positive (+) DME with KOI-{ al 40171 and heat, than with the calcofluor white and Fluorescencc Technique. an841/0 and 9014 respectively.
Through the laiter, a higher amouni of elcmcnts with fungi (hifas, macro and microconidias) were observed, and it vas posible to ci cari y see the morph ol ogical charactcristics of ihese elements.
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Monheit EJ.; MD Cowan D.F.; MD Moore D.Cj PhD Rapid detection of fungi li tissucs using calcofluor White and Fluorescence Microscopy. Arch Pathol E.ah Mcci Vol: 108. 616 —618 August 1984.
Kwon—Chung. K.J. & Benett J.E. Medical Mycology p. 118 Ed. Lea and Fehiger Phi lade lphya - London 1992.
R.Rüchel and S.Margraf. Rapid microscopical diagnosis of deep - seated mycosis foliowing maceration of frcsh Specimens and Staining with optical brighteners. Mycoses 36, 239-242. 1993.
Darken M: Applications of fluorescent brighteners in biological techniques. Science 1961; 133: 1704—OS.
Rippon, J.W. Medical Mycology, Philadelphia: W.B Saunders. The Pathogenic Fungi and the Pathogenic Actinomycetes, 3 rd edn. 1988.
Panu H. Hendolin, Lars Paulin, Pirkko Koukila - Kahkola, Ve¡¡ - Jukka Anttila, Hcnrik Malmberg, Ma]colm Richardson and Jukka Ylikaski. Panfungal PCR and Multipiex Liqued Hybridization for detection of fungi in tissue speclmens. Journal of clinical Microbiology p4186 - 4192 Nov. 2000.
Ramos, L.; Riconi, A. Bracalente, B. C. Uso del Blancophor (Fluorocromo) en la búsqueda de hongos en materiales de piel por microscopia directa . Rey. Ibero Am. Micol.7: 107-110.1990.
Chander , J. ; Chakraberti , A. ; Sharrna A.;Saini ,J.S. ,Panigahi ,D.Evaluationof calcofluorstaining in the diagnosis of fungal corneal ulcer. Mycosis 36: 243-245. 1993.
legeage, G.J.; Harrington, D.J. Use of calcofluor white in clinical Mycology Lab. Med. 15: 109-115. 1994.
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