Determination of some oxidative stress, functional and immunological markers in the saliva of patients undergoing bone marrow transplantation (BMT).
DOI:
https://doi.org/10.31053/1853.0605.v78.n4.33227Keywords:
Bone Marrow Transplantation, Saliva, Biomarkers, Oxidative StressAbstract
OBJECTIVE: high doses of chemotherapy used prior to bone marrow transplantation (BMT) promote severe changes in the stomatognathic system. The objective of the present work consisted in evaluating some functional, immunological and oxidative stress markers in saliva of these patients.
METHODS: a longitudinal observational study was carried out on 22 patients admitted to the Bone Marrow Transplant Unit of the Oncohematology Service of the Sanatorio Allende between March 2019 and February 2020. Basal saliva collection was carried out in the initial stage (I ) prior to isolation and middle stage (M) 14 days after conditioning therapy and transplantation. The concentration of uric acid (UA), superoxide dismutase (SOD), malondialdehyde (MDA), salivary alpha amylase, secretory immunoglobulin A (Ig As), lactoferrin, ceruloplasmin and urea were analyzed.
RESULTS: in (M) the levels of SOD and MAD increased significantly compared to (I) (p <0.01). The concentration of salivary alpha amylase, Ig As, lactoferrin and uric acid was significantly lower in (M) compared to (I ) p <0.0001, p <0.01, p <0.0001, p <0.02 respectively. Ceruloplasmin and Urea did not show variations during treatment.
CONSLUSION: in the present study, a decrease in the defensive capacity of saliva was observed as a consequence of a reduction in the concentration of Ig As and lactoferrin. The increase in SOD in (M) could be interpreted as a defense mechanism of saliva against oxidative stress produced by chemotherapy. The decrease in uric acid in stage (M) could allow the worsening of mucositis. The synthesis and release of amylase was affected by treatment with cytostatic drugs.
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Razzaghdoust A, Mofid B, Moghadam M. Development of a simplified multivariable model to predict neutropenic complications in cancer patients undergoing chemotherapy. Support Care Cancer. 2018 Nov;26(11):3691-3699. doi: 10.1007/s00520-018- 4224-z.
Svilaas T, Lefrandt JD, Gietema JA, Kamphuisen PW. Long-term arterial complications of chemotherapy in patients with cancer. Thromb Res. 2016 Apr;140 Suppl 1:S109-18. doi: 10.1016/S0049- 3848(16)30109-8.
Basile D, Di Nardo P, Corvaja C, Garattini SK, Pelizzari G, Lisanti C, Bortot L, Da Ros L, Bartoletti M, Borghi M, Gerratana L, Lombardi D, Puglisi F. Mucosal Injury during Anti-Cancer Treatment: From Pathobiology to Bedside. Cancers (Basel). 2019 Jun 20;11(6):857. doi: 10.3390/cancers11060857.
Mazzeo MA, Linares JA, Campos ML, Busamia BE, Dubersarsky C, Lavarda M, Jarchum G, Finkelberg AB. Oral signs of intravenous chemotherapy with 5-Fluorouracil and Leucovorin calcium in colon cancer treatment. Med Oral Patol Oral Cir Bucal. 2009 Mar 1;14(3):E108-13.
Jensen SB, Pedersen AM, Reibel J, Nauntofte B. Xerostomia and hypofunction of the salivary glands in cancer therapy. Support Care Cancer. 2003 Apr;11(4):207-25. doi: 10.1007/s00520-002-0407-7.
Nieuw Amerongen AV, Veerman EC. Current therapies for xerostomia and salivary gland hypofunction associated with cancer therapies. Support Care Cancer. 2003 Apr;11(4):226-31. doi: 10.1007/s00520-002-0409-5.
Yoshizawa JM, Schafer CA, Schafer JJ, Farrell JJ, Paster BJ, Wong DT. Salivary biomarkers: toward future clinical and diagnostic utilities. Clin Microbiol Rev. 2013 Oct;26(4):781-91. doi: 10.1128/CMR.00021-13.
Tóthová L, Kamodyová N, Červenka T, Celec P. Salivary markers of oxidative stress in oral diseases. Front Cell Infect Microbiol. 2015 Oct 20;5:73. doi: 10.3389/fcimb.2015.00073.
Salum FG, Medella Junior FAC, Figueiredo MAZ, Cherubini K. Salivary hypofunction: an update on therapeutic strategies. Gerodontology. 2018 Dec;35(4):305-316. doi: 10.1111/ger.12353.
Hershkovich O, Nagler RM. Biochemical analysis of saliva and taste acuity evaluation in patients with burning mouth syndrome, xerostomia and/or gustatory disturbances. Arch Oral Biol. 2004 Jul;49(7):515-22. doi: 10.1016/j.archoralbio.2004.01.012.
González-Mangado N, Morera Prat J. Oxidación celular y fármacos mucoactivos antioxidantes [Cell oxidative processes and antioxidant mucoactive drugs]. Arch Bronconeumol. 2001 Nov;37(10):407-10. Spanish. doi: 10.1016/s0300-2896(01)75109- 5.
Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39(1):44-84. doi: 10.1016/j.biocel.2006.07.001.
Iannitti T, Rottigni V, Palmieri B. Role of free radicals and antioxidant defences in oral cavity-related pathologies. J Oral Pathol Med. 2012 Oct;41(9):649-61. doi: 10.1111/j.1600- 0714.2012.01143.x.
Schafer CA, Schafer JJ, Yakob M, Lima P, Camargo P, Wong DT. Saliva diagnostics: utilizing oral fluids to determine health status. Monogr Oral Sci. 2014;24:88-98. doi: 10.1159/000358791
Farnaud SJ, Kosti O, Getting SJ, Renshaw D. Saliva: physiology and diagnostic potential in health and disease. ScientificWorldJournal. 2010 Mar 16;10:434-56. doi: 10.1100/tsw.2010.38.
Buczko P, Zalewska A, Szarmach I. Saliva and oxidative stress in oral cavity and in some systemic disorders. J Physiol Pharmacol. 2015 Feb;66(1):3-9.
Battino M, Ferreiro MS, Gallardo I, Newman HN, Bullon P. The antioxidant capacity of saliva. J Clin Periodontol. 2002 Mar;29(3):189-94. doi: 10.1034/j.1600-051x.2002.290301x.x.
Martinez JM, Pereira D, Chacim S, Mesquita E, Sousa I, Martins Â, Azevedo T, Mariz JM. Mucositis care in acute leukemia and non- Hodgkin lymphoma patients undergoing high-dose chemotherapy. Support Care Cancer. 2014 Sep;22(9):2563-9. doi: 10.1007/s00520-014-2199-y.
Mazzeo MA, Linares JA, López MM, Bachmeier E, Wietz FM, Galván V, Valentinuzzi MC, Riveros JA, Finkelberg A. Analysis of saliva samples from oncological patients treated with 5-fluorouracil and leucovorin calcium by scanning electron microscopy with energy dispersive system. J Oral Pathol Med. 2013 Nov;42(10):788-92. doi: 10.1111/jop.12078.
Bachmeier E, Mazzeo MA, López MM, Linares JA, Jarchum G, Wietz FM, Finkelberg AB. Mucositis and salivary antioxidants in patients undergoing bone marrow transplantation (BMT). Med Oral Patol Oral Cir Bucal. 2014 Sep 1;19(5):e444-50. doi: 10.4317/medoral.19062.
Vedam VKV, Boaz K, Natarajan S, Ganapathy S. Salivary Amylase as a Marker of Salivary Gland Function in Patients Undergoing Radiotherapy for Oral Cancer. J Clin Lab Anal. 2017 May;31(3):e22048. doi: 10.1002/jcla.22048.
de Sousa-Pereira P, Woof JM. IgA: Structure, Function, and Developability. Antibodies (Basel). 2019 Dec 5;8(4):57. doi: 10.3390/antib8040057.
Drago-Serrano ME, Campos-Rodríguez R, Carrero JC, de la Garza M. Lactoferrin: Balancing Ups and Downs of Inflammation Due to Microbial Infections. Int J Mol Sci. 2017 Mar 1;18(3):501. doi: 10.3390/ijms18030501.
Shah PH, Venkatesh R, More CB. Determination of role of ceruloplasmin in oral potentially malignant disorders and oral malignancy-A cross-sectional study. Oral Dis. 2017 Nov;23(8):1066-1071. doi: 10.1111/odi.12690.
Akinmoladun VI, Arinola OG, Elumelu-Kupoluyi T, Eriba LO. Evaluation of humoral immunity in oral cancer patients from a nigerian referral centre. J Maxillofac Oral Surg. 2013 Dec;12(4):410-3. doi: 10.1007/s12663-012-0440-0.
Aguilar Cordero MJ, González Jiménez E, Perona JS, Alvarez Ferre J, Padilla López CA, Rivas García F, Katarzyna P, Ocete Hita
E. Ceruloplasmina y su importancia clínica como factor indicador del riesgo cardiovascular en una población de escolares de Granada [Ceruloplasmin and its clinical relevance as an indactor of cardiovascular risk factor in a school population of Granada]. Nutr Hosp. 2011 May-Jun;26(3):655-8. Spanish. doi: 10.1590/S0212- 16112011000300033.
Bilancio G, Cavallo P, Lombardi C, Guarino E, Cozza V, Giordano F, Palladino G, Cirillo M. Salivary levels of phosphorus and urea as indices of their plasma levels in nephropathic patients. J Clin Lab Anal. 2018 Sep;32(7):e22449. doi: 10.1002/jcla.22449.
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