Elicobacter pylori detected in atheroma plaque
Keywords:
Helicobacter pylori, atherosclerosis, atherorna plaque, autoimmunityAbstract
Introduction: In general, infection and chronic inflamination have been implied as etiologie agenls for atheroesclerosis and in particular eoronary illness (CI). Several studies have correlated the infeetion of Helicobacter pylori with CI, especially with virulent strains (lineage Cag A). Objective: Demonstrate the immunohistochemical presence of H. Pylori in atheroseletotic plaques obtained from endarterectomy of different vascular regions. Material and rnethods: 34 atherosclerotic plaques of different vascular arcas were studied, (25 men and 9 women). The tissues were fixed with 10% neutral buffered-formalin and decalcifying in formlc acid 5% was used when necessary. The tissue sections were included in paraffin, cut and colored with H&E and subjected to Immunohistochemistry (IHC) of H.Pylori. Briefly, tissues were deparaffinized and thermally treated with a citrate-based solution of antigenic retrieval (ImmunoDNA Retriever with Citrate, BIO SB, Santa Barbara, CA) using a water bath at 95°C for 1 hour. The IHC was conducted using a high sensiUvity Biotin- Streptavidin-HRP-DAB IHC system (ImmunoDetector HRP/DAB, BIO SB). The rnicroscopic observaUon evaluated the presence of mononuclear inflamrnatory celis and the identification of the bacteria in the wall or the vascular lumen. Results: Of the 34 cases studied 14 were positive, where one could identify the bacillus tu their different forms (41,17%) associated with chronic inflammation.
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