First evidence of Bnyamwera orthobunyavirus circulation in Formosa province, Argentina

Authors

  • R Gallardo Instituto de Virología Dr J M Vanella, FCM, UNC
  • G Albrieu Instituto de Virología Dr J M Vanella, FCM, UNC
  • M Laurito Centro de investigaciones entomológicas de Córdoba CIEC. Instituto de Investigaciones Biológicas y Tecnológicas (IIBYT) CONICET- FCEFyN, UNC
  • A Mansilla Instituto de Ciencias de la Tierra y Ambientales de La Pampa (INCITAP). Centro para el Estudio y Conservación de las Aves Rapaces en Argentina (CECARA)
  • M Beranek Instituto de Medicina Regional. Universidad Nacional del Nordeste.
  • M Contigiani Instituto de Virología Dr J M Vanella. FCM. UNC
  • L Spinsanti Instituto de Virología Dr J M Vanella. FCM. UNC

Keywords:

Bunyamwera Orthobunyavirus virus, Arbovirus, Mosquitoes, Formosa

Abstract

Bunyamwera orthobunyavirus (BUNV) virus is an arbovirus kept in nature in a cycle that mainly involves mosquitoes and vertebrates. In America, this virus is important because of its potential pathogenicity for both human and domestic animals. In Argentina, Aedes (Ochlerotatus) albifasciatus (Córdoba) and Psorospora (Grabhamia) varinervis (Santa Fe) mosquito strains have been recovered; and from horses with neurological syndrome and an equine abortion (Santa Fe). Between 2008-2009 and 2013-2014, mosquitoes of other species infected by BUNV were detected in the Center and Northeast of the province of Córdoba, their involvement in public health being unknown. In Córdoba, the presence of neutralizing antibodies in humans was demonstrated, and a clinical case was confirmed by BUNV with febrile syndrome reported as Dengue during the outbreak that produced this virus in 2009.

The objective of this work was to know the presence / absence of BUNV in mosquitoes in the town of San Francisco Laishí in Formosa province during the autumn of 2017.

We analyzed 21 pools, each consisting of 50 individuals per species of mosquitoes, previously identified and collected in a field in the San Francisco Laishí. BUNV was detected by generic RT-PCR for orthobunyavirus that amplifies a region of 251 (base pairs) of genomic segment S and subsequent sequencing and phylogenetic analysis.

Of the total pools analyzed, 6 were obtained with a positive result. The positive pools corresponded to the species Culex (Melanoconion) educator (2), Cx (Mel) bastagarius, Cx (Mel) intrincatus, Ae (Och) Sp, Cx (cux) acharistus. Five samples were sequenced, which were grouped together with those previously determined in the same clade.

These preliminary results, experienced a contribution to knowledge about the circulation of the Orthobunyavirus genus in Argentina and encourage us to continue research regarding the role of BUNV as a human and / or domestic animal pathogen and its maintenance cycle.

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Published

2019-10-15

How to Cite

1.
Gallardo R, Albrieu G, Laurito M, Mansilla A, Beranek M, Contigiani M, Spinsanti L. First evidence of Bnyamwera orthobunyavirus circulation in Formosa province, Argentina. Rev Fac Cien Med Univ Nac Cordoba [Internet]. 2019 Oct. 15 [cited 2024 Jul. 19];76(Suplemento). Available from: https://revistas.unc.edu.ar/index.php/med/article/view/25755

Issue

2019: Suplemento JIC XX

Section

Otros

License

The Faculty of Medical Sciences Journal (RFCM) subscribes to the Open Access policy and does not charge authors fees for publishing, nor does it charge readers fees for accessing published articles (APC).