Protocolo para la preparación de secciones delgadas sin descalcificar de tejido óseo humano

Tamara Giselle Navarro; Marcos Jannello; Ignacio A. Cerda; Marien Béguelin; Romina Vázquez

doi:10.31048/1852.4826.v11.n1.17007

Authors

Tamara Giselle Navarro Museo Provincial Carlos Ameghino
Marcos Jannello Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales
Ignacio A. Cerda Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación en Paleobiología y Geología
Marien Béguelin Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación en Paleobiología y Geología
Romina Vázquez Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación en Paleobiología y Geología

DOI:

https://doi.org/10.31048/1852.4826.v11.n1.17007

Keywords:

protocol, thin section, human bone remains, Histology

Abstract

Making thin sections from fossil and archaeological materials is usually a highly time consuming and expensive task. Also, the creation of a laboratory with necessary characteristics for the development of established protocols is also expensive. This paper presents an alternative protocol for obtaining thin sections of human bone samples from archaeological sites with the aim of applying it to microstructural analysis. The procedures for obtaining thin cuts that are detailed here are within the reach of newly formed laboratories. In the first place a femur, a tibia and a first rib n adult individual from a contemporary cemetery was measured and recorded with digital images. Fragments of 1 cm of bone were them extracted. Molds and casts of the extracted samples were prepared before thin section preparation. The obtained results were optimal for the observation of the microstructural bone features. With the appropriate modifications (set time, abrasive grading according to the hardness of the material, impregnation, etc.) this protocol could be useful for several kinds of samples (extant, archaeological and even fossil ones).

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Author Biographies

Marcos Jannello, Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales

Museo de Historia Natural de San Rafael.

Ignacio A. Cerda, Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación en Paleobiología y Geología

Sede Alto Valle-Valle Medio. Universidad Nacional de Río Negro. Museo Provincial Carlos Ameghino.

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