Polyclonal antiserum for the detection of Cymbidium mosaic virus and Odontoglossum ringspot virus Production
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Abstract
Orchids (family Orchidaceae) are susceptible to several biotic and abiotic factors that affect their development and quality. Among them, Cymbidium mosaic virus (CymMV) and Odontoglossum ingspot virus (ORSV) are the most frequent pathogens, with the greatest economic impact worldwide. The mechanical transmission of these viruses in addition to the predominantly agamic multiplication of orchids, make it necessary to work with virus-free plant material. The objective of this work was to develop diagnostic reagents for the detection of CymMV and ORSV. A polyclonal antiserum against CymMV + ORSV was made with a titer of 1:10000 determined by NC-ELISA. The antiserum was processed generating IgG and IgG-conjugated” anti CymMV+ORSV were obtained for use in DAS-ELISA in a dilution of 1:500. The immunodiagnostic
capacity of this technique was evaluated by analyzing samples from orchids with characteristic viral symptoms, collected from commercial nurseries in the city of Córdoba, Argentina. Viral infections were detected in 22 % of the samples analyzed and in 75 % of the nurseries surveyed. The ability of these reagents to identify CymMV, ORSV and mixed infections in orchids, will speed up analyzes and reduce their costs. This would represent an advantage for large-scale detections.
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References
Arditti, J. y Ernst, R. (1993). Micropropagation of Orchids. New York, EE.UU.: John Wiley & Sons.
Cánovas, S. E, Ballari, M. C. y Nome, C. F. (2016). First report of Cymbidium mosaic virus and
Odontoglossum ring spot virus in Argentina. Australasian Plant Disease Notes, 11, 2. https://doi.org/10.1007/s13314-015-0189-7
Clark, M. F. y Adams, A. N. (1977). Characteristics of the microplate method of enzyme linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34(3), 475-483.
Freitas-Astua, J., Rezende, J. A. M. y Kitajima, E. W. (1999). Incidence of orchid viruses in the state of Sao Paulo, Brazil. Fitopatologia Brasileira, 24,125-130.
Freuler, M. J. (2007). Orquídeas. Buenos Aires, Argentina: Editorial Albatros.
Hartmann, H. T. y Kester, D. E. (1991). Propagación de plantas: principios y prácticas. Distrito Federal, México: Editorial Continental.
Hu, J. S., Ferreira, S., Wang, M. y Xu, M. Q. (1993). Detection of Cymbidium mosaic virus, Odontoglossum ringspot virus, Tomato spotted wilt virus, and potyviruses infecting orchids in Hawaii. Plant Disease, 77, 464-468. https://doi.org/10.1094/PD-77-0464
Kassanis, B. (1957). Effects of changing temperature on plant virus diseases. Advance in Virus Research, 4, 221-241. https://doi.org/10.1016/S0065-3527(08)60600-4
Kitajima, E. W. y Nome, C. F. (1999). Microscopía electrónica en virología vegetal. En: Docampo, D. M., y Lenardon, S. L. (Eds.), Métodos para detectar patógenos sistémicos (59-87). Córdoba, Argentina: INTA-JICA.
Lefkowitz, E. J, Dempsey, D. M., Hendrickson, R. C., Orton, R. J, Siddell, S. G. y Smith, D. B. (2018). Virus taxonomy: the database of the International Committee on Taxonomy of Viruses (ICTV). Nucleic Acids Research, 46 (D1), D708-D717. https://doi.org/10.1093/nar/gkx932
Obrepalska-Steplowska, A., Renaut, J., Planchon, S., Przybylska, A., Wieczorek, P., Barylski, J., Palukaitis, P. (2016). Effect of temperature on the pathogenesis, accumulation of viral and satellite RNAs and on plant proteome in peanut stunt virus and satellite RNA-infected plants: Corrected version. Frontiers in Plant Science7, 839. https://doi.org/10.3389/fpls.2016.00839
Pant, R., Das, M., Pun, K., Ramachandran, P. y Medhi, R. (2010). Occurrence of Cymbidium mosaic and Odontoglossum ringspot viruses in orchid germplasm of Sikkim and Darjeeling hills, their identification and diagnosis. Indian Phytopathology, 63, 326-332.
Pant, R. P., Gupta, S., Hakmoasa, B., Meena, N. K., Vij, S. P. y Medhi, R. P. (Marzo 2013). Status of orchid virus diagnostics and their application in production of virus free planting material. En: R. P. Medhi, R. Devadas, N. Sailo, N. G. Debnath, Jaya Gogoi, S. L. Pattanayak (Eds.), National Dialogue on Orchid Conservation & Sustainable Development for Community Livelihood (61-71). Gangtok, Sikkim, India: National Research Centre for Orchids, Indian Council of Agricultural Rese arch.
Parent, J. G., Berlanger, F., Desjardins, S. y Brisson, J. D. (1985).. Dot-immunobinding for detection of tomato mosaic virus and potato virus X, infecting greenhouse tomatoes. Phytoprotection, 66, 53-57.
Pearson, M. N. y Cole, J. S. (1986). The effects of Cymbidium mosaic virus and Odontoglossum ringspot virus on the growth of Cymbidium orchids. Journal of Phytopathology, 117(3), 193-197. https://doi.org/10.1111/j.1439-0434.1986.tb00934.x
Pradhan, S., Regmi, T., Ranjit, M. y Pant, B. (2016). Production of virus-free orchid Cymbidium aloifolium (L.) Sw. by various tissue culture techniques. Heliyon, 2 (10), e00176. https://doi.org/10.1016/j.heliyon.2016.e00176
Rani, P., Pant, R. P. y Jain, R. K. (2010). Serological detection of Cymbidium mosaic and Odontoglossum ringspot viruses in orchids with polyclonal antibodies produced against their recombinant coat proteins. Journal of Phytopathology, 158 (7-8), 542-545. https://doi.org/10.1111/j.1439-0434.2009.01668.x
Simone, G.W. y Burnett, H.C. (2002). Diseases caused by bacteria and fungi. En: J. Watson (Ed.), Orchid Pests and Diseases (50–73). Delray Beach, Florida, Estados Unidos: American Orchid Society.
Tanaka, S., Nishii, H., Ito, S., Kameya-Iwaki, M. y Sommartya, P. (1997). Detection of Cymbidium mosaic potexvirus and Odontoglossum ringspot tobamovirus from Thai orchids by rapid immunofilter paper assay. Plant Disease, 81(2), 167-170. https://doi.org/10.1094/PDIS.1997.81.2.167
Wong, S. M., Chng, C. G., Lee, Y. H., Tan, K. y Zettler, F. W. (1994). Incidence of Cymbidium mosaic and Odontoglossum ringspot viruses and their significance in orchid cultivation in Singapore. Crop Protection, 13 (3), 235-239. https://doi.org/10.1016/0261-2194(94)90084-1
Zheng, Y.-X., Shen, B.-N., Chen, C.-C. y Jan, F.-J. (2010). Odontoglossum ringspot virus causing flower crinkle in Phalaenopsis hybrids. European Journal of Plant Pathology, 128, 1-5. https://doi.org/10.1007/s10658-010-9630-0
Zettler, F. W., Ko, N. J., Wisler, G. C., Elliott, M. S., y Wong, S. M. (1990). Viruses of orchids and their control. Plant Disease, 74(9), 621-626. https://doi.org/10.1094/PD-74-0621