Citrullus lanatus: a new natural host of Groundnut ringspot orthotospovirus in Argentina
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Orthotospoviruses cause serious economic damage, being a major limitation for production and quality in different crops. The objectives of this work were to detect the presence of orthotospovirus that are infecting cucurbit crops in the country and analyze its phylogeny. Samples were taken in watermelon (Citrullus lanatus) production lots during 2017/2018 in Santiago del Estero province. The results of this work showed in the watermelon samples showed the presence of characteristic virions of the Orthotospovirus genus, and were positive to serological and molecular tests with specific reagents (DAS-ELISA and RTPCR) confirming the presence of groundnut ringspot orthotospovirus (GRSV). Genomic fragments were amplified, sequenced and deposited in GenBank, access nº MK680832 and MK680833. Phylogenetic analysis of N-protein nucleotide partial sequences showed that GRSV isolates from Argentina (watermelon host) were grouped into a new group with high bootstrap value. These sequences were placed in a cluster formed by sequences from the American continent that were separated from those that conformed the group of sequences of African origin. This work is the first report of the presence of the GRSV in watermelon (Citrullus lanatus) cultivation in Argentina.
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References
Bag, S., Mitter, N., Eid, S. y Pappu, H. R. (2012). Complementation between Two Tospoviruses Facilitates the Systemic Movement of a Plant Virus Silencing Suppressor in an Otherwise Restrictive Host. PLoS ONE, 7 (10), e44803. DOI: https://doi.org/10.1371/journal.pone.0044803
Bezerra, I. C., Resende, R. O., Pozzer, L., Nagata, T., Kormelink, R. y De Avila, A. C. (1999). Increase of tospoviral diversity in Brazil with the identification of two new Tospovirus species, one from chrysanthemum and one from zucchini. Phytopathology,89 (9), 823-830. DOI: https://doi.org/10.1094/phyto.1999.89.9.823
Basic Local Alignment Search Tool. [Software]. Maryland, EE.UU.: U.S. National Libarary of Medicine. National Center for Biotechnology Information. https://blast.
ncbi.nlm.nih.gov/Blast.cgi.
Boari, A. J., Maciel-Zambolim, E., Lau, D. D., Lima, G. S. A., Kitajima, E. W., Brommonschenkel, S. S. H. y Zerbini, F. M. (2002). Detection and partial
characterization of an isolate of Groundnut ringspot virus in Solanum sessiliflorum. Fitopatologia Brasileira, 27 (3), 249–253. DOI: https://doi.org/10.1590/S0100-41582002000300002
Ciuffo, M., Kurowski, C., Vivoda, E., Copes, B., Masenga, V., Falk, B. W. y Turina, M. (2009). A new Tospovirus sp. in cucurbit crops in Mexico. Plant Disease, 93 (3), 467-474. DOI: https://doi.org/10.1094/PDIS-93-5-0467
Clark, M. F. y Adams, A. N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of
General Virology,34 (3), 475-483. DOI: https://doi.org/10.1099/0022-1317-34-3-475
de Avila, A. C., de Haan, P., Kormelink, R., Resende, R. O., Goldbach, R. W. y Peters, D. (1993). Classification of tospoviruses based on phylogeny of nucleoprotein gene sequences. Journal of General Virology, 74 (2), 153–159. DOI: https://doi.org/10.1099/0022-1317-74-2-153
de Breuil, S., Abad, J. A., Nome, C. F., Giolitti, F. J., Lambertini, P. L. y Lenardon, S. (2007). Groundnut ringspot virus: an emerging tospovirus inducing disease in peanut crops. Journal of Phytopathology,155 (2), 251–254. DOI: https://doi.org/10.1111/j.1439-0434.2007.01221.x
Dewey, R. A., Semorile, L.C. y Grau, O. (1996). Detection of Tospovirus species by RT-PCR of the N-gene and restriction enzyme digestions of the products. Journal of Virological Methods, 56, 19–26. DOI: https://doi.org/10.1016/0166-0934(95)01896-4
Gambino, G., Perrone, I. y Gribaudo, I. (2008). A rapid and effective method for RNA extraction from different tissues of grapevine and other woody plants. Phytochemical Analysis, 19 (6), 520-525. DOI: https://doi.org/10.1002/pca.1078
German, T., Ullman, D. y Moyer, J. (1992). Tospoviruses: Diagnosis, Molecular Biology, Phylogeny, and Vector Relationships. Annual Review of Phytopathology, 30, 315–348. DOI: https://doi.org/10.1146/annurev.py.30.090192.001531
Goldbach, R. y Kuo, G. (1996). Introduction. Acta Horticulturae, 431, 21-26. DOI: https://doi.org/10.17660/ActaHortic.1996.431.1
Gracia, O. y Feldman, J. M. (1986). Virus identificados en cultivos de cucurbitáceas. IDIA 445-448, 1-6.
Holkar, S. K., Mandal, B., Reddy, M. K. y Jain, R. K. (2019). Watermelon bud necrosis orthotospovirus. An emerging constraint in the Indian subcontinent: An overview. Crop Protection, 117, 52-62. DO69+I: https://doi.org/10.1016/j.cropro.2018.11.005
Jain, R. K., Pappu, H. R., Pappu, S. S., Reddy, M. K. y Vani, A. (1998). Watermelon bud necrosis tospovirus is a distinct virus species belonging to serogroup IV. Archives of Virology, 143, 1637-1644. DOI: https://doi.org/10.1007/s007050050405
Jones, D. R. (2005). Plant viruses transmitted by thrips. European Journal of Plant Pathology, 113, 119–157. DOI: https://doi.org/10.1007/s10658-005-2334-1
Kato, K., Handa, K. y Kameya-Iwaki, M. (2000). Melon yellow spot virus: a distinct species of the genus Tospovirus isolated from melon. Phytopathology, 90 (4), 422-426. DOI: https://doi.org/10.1094/PHYTO.2000.90.4.422
Kumar, S., Stecher, G. y Tamura, K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. (version 7.0) [Software]. Recuperado de: https://www.megasoftware.net/
Leão, E., Spadotti, D., Rocha, K., Pantoja, K., Rezende, J., Pavan, M., y Krause Sakate, R. (2015). Citrullus lanatus is a new natural host of Groundnut ringspot virus in Brazil. Journal of Phytopathology, 163 (11-12), 1014-1018. DOI: https://doi.org/10.1111/jph.12327
Lecoq, H. y Katis, N. (2014). Control of cucurbit viruses. Advances in Virus Research, 90, 255-296. DOI: https://doi.org/10.1016/B978-0-12-801246-8.00005-6
Loebenstein, G. y Lecoq, H. (Eds.) (2012). Viruses and virus diseases of vegetables in the Mediterranean basin. (Vol. 84). Estados Unidos: Academic Press.
Maes, P., Adkins, S., Alkhovsky, S. V., Avšič-Županc, T., Ballinger, M. J., Bente, D. A., …y Kuhn, J. H. (2019). Taxonomy of the order Bunyavirales: second update 2018. Archives of Virology, 164, 927-941. DOI: https://doi.org/10.1007/s00705-018-04127-3
Michelotto, M., Carrega, W., Lamana, L., de Souza, T., de Godoy, I., dos Reis, L., ... y Carvalho, R. (2019). Losses caused by Groundnut ringspot tospovirus in peanut crop in the State of São Paulo. Semina. Ciências Agrárias, 40 (6), 3429-3442. DOI: http://dx.doi.org/10.5433/1679-0359.2019v40n6Supl3p3429
Milne,J. R., Khumlekhasing, M. y Walter, G. H. (1996). Understanding host plant relationships of polyphagous flower thrips, a case study of Frankliniella schultzei
(Trybom). En S. Goodwin and P. Gillespie (Eds.), Proceedings of the 1995 Australia and New Zealand Thrips Workshop: Methods, Ecology and Management) (pp. 8–14). Gosford, Australia: New South Wales Agriculture.
Mumford, R. A., Barker, I. y Wood, K. R. (1996). The biology of the tospoviruses. Annals of Applied Biology, 128, 159-183. DOI: https://doi.org/10.1111/j.1744-7348.1996.tb07097.x
Nagata, T., Almeida, A. C. L., Resende, R. D. O. y De Ávila, A. C. (2004). The competence of four thrips species to transmit and replicate four tospoviruses. Plant Pathology, 53 (2), 136-140. DOI: https://doi.org/10.1111/j.0032-0862.2004.00984.x
Nome, S. F., March, G. J. y Giorda, L. M. (1974). Disminución de la productividad de plantas de zapallito de tronco infectadas con el virus del mosaico de la sandía, raza 2 (Watermelon Mosaic Virus-2). IDIA 321-324, 26-31.
Olaya, C., Adhikari, B., Raikhy, G., Cheng, J. y Pappu, H. R. (2019). Identification and localization of Tospovirus genus-wide conserved residues in 3D models of the nucleocapsid and the silencing suppressor proteins. Virology Journal, 16, 7. DOI: ttps://doi.org/10.1186/s12985-018-1106-4
Oliver, J. E. y Whitfield, A. E. (2016). The Genus Tospovirus: Emerging Bunyaviruses that Threaten Food Security. Annual Review of Virology, 3, 101-124. DOI: https://doi.org/10.1146/annurev-virology-100114-055036
Pappu, H. R., Jones, R. A. C. y Jain, R. K. (2009). Global status of tospovirus epidemics in diverse cropping systems: successes achieved and challenges ahead. Virus Research, 141 (2), 219-236. DOI: https://doi.org/10.1016/j.virusres.2009.01.009
Perotto, M. C., Celli, M. G., Pozzi, E. A., Luciani, C. E. y Conci, V. C. (2016). Occurrence and characterization of a severe isolate of Watermelon mosaic virus from Argentina. European Journal of Plant Pathology, 146, 213-218. DOI: https://doi.org/10.1007 /s10658-016-0904-z
Perotto, M. C., Pozzi, E. A., Celli, M. G., Luciani, C. E., Mitidieri, M. S. y Conci, V. C. (2018). Identification and characterization of a new potyvirus infecting cucurbits. Archives of Virology, 163, 719-724. DOI: https://doi.org/10.1007/s00705-017-3660-2
Plyusnin, A., Beaty, B. J., Elliott, R. M., Goldbach, R., Kormelink, R., Lundkvist, A.,… y Tesh, R .B. (2011). Bunyaviridae. In A. M. Q. King, M. J. Adams, E. B. Carstens, E. J. Lefkowitz (Eds.), Ninth Report of the International Committee on Taxonomy of Viruses. Recuperado de: https://talk.ictvonline.org/ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/negrna_viruses/205/bunyaviridae
Pozzi, E. A., Luciani, C. E., Celli, M. G., Conci, V. C., y Perotto, M. C. (2019). First Report of Zucchini Lethal Chlorosis Virus in Argentina Infecting Squash Crops. Plant Disease, 104 (2), 609. DOI: https://doi.org/10.1094/PDIS-05-19-1064-PDN
Pretty, J. (2007). Agricultural sustainability: concepts, principles and evidence. Philosophical Transactions of the Royal Society B: Biological Sciences, 363 (1491),
DOI: https://doi.org/10.1098/rstb.2007.2163
Rao, X., Liu, Y., Wu, Z., y Li, Y. (2011). First report of natural infection of watermelon by Watermelon silver mottle virus in China. New Disease Reports, 24, 2044-0588. DOI: https://doi.org/10.5197/j.2044-0588.2011.024.012
Riley, D. G., Joseph, S. V., Srinivasan, R. y Diffie, S. (2011). Thrips Vectors of Tospoviruses. Journal of Integrated Pest Management, 2, I1–I10. DOI: https://doi.org/10.1603/IPM10020
Rotenberg, D., Jacobson, A. L., Schneweis, D. J. y Whitfield, A. E. (2015). Thrips transmission of tospoviruses. Current Opinion in Virology, 15, 80-89. DOI: https://doi.org/10.1016/j.coviro.2015.08.003
Sistema Nacional Argentino de Vigilancia y Monitoreo de plagas (SINAVIMO) (2019a). Frankliniella schultzei. Recuperado de https://www.sinavimo.gov.ar/plaga/frankliniella-schultzei
Sistema Nacional Argentino de Vigilancia y Monitoreo de plagas (SINAVIMO) (2019 b). Frankliniella occidentalis. Recuperado de https://www.sinavimo.gov.ar/plaga/frankliniella-occidentalis
Sistema Nacional Argentino de Vigilancia y Monitoreo de plagas (SINAVIMO) (2019 c). Groundnut ring spotvirus (GRSV). Recuperado de https://www.sinavimo.gov.ar/plaga/groundnut-ring-spot-virus
Spadotti, D., Leão, E., Rocha, K., Pavan, M. y Krause-Sakate, R. (2014). First report of Groundnut ringspot virus in cucumber fruits in Brazil. New Disease Reports,29, 25. DOI: http://dx.doi.org/10.5197/j.2044-0588.2014.029.025
Tsompana, M., Abad, J., Purugganan, M., y Moyer, J. W. (2005). The molecular population genetics of the Tomato spotted wilt virus (TSWV) genome. Molecular
Ecology, 14, 53-66. DOI: https://doi.org/10.1111/j.1365-294X.2004.02392.x
Turina, M., Tavella, L. y Ciuffo, M. (2012). Tospoviruses in the Mediterranean area. Advances in Virus Research, 84, 403-437. DOI: https://doi.org/10.1016/B978-0-12-394314-9.00012-9
Webster, C. G., Frantz, G., Reitz, S. R., Funderburk, J. E., Mellinger, H. C., McAvoy, E., …. y Adkins, S. (2015). Emergence of Groundnut ringspot virus and Tomato chlorotic spot virus in vegetables in Florida and the southeastern United States. Phytopathology,105 (3), 388-398. DOI: https://doi.org/10.1094/PHYTO-06-14-0172-R
Wijkamp, I., Almarza, N., Goldbach, R. y Peters, D. (1995). Distinct levels of specificity in thrips transmission of tospoviruses. Phytopathology,85 (10), 1069-1074. DOI: https://doi.org/10.1094/Phyto-85-1069
Yeh, S. D., Lin, Y. C., Cheng, Y. H., Jih, C. L., Chen, M. J. y Chen, C. C. (1992). Identification of tomato spotted wilt-like virus on watermelon in Taiwan. Plant Disease, 76, 835-840. DOI: https://doi.org/10.1094/PD-76-0835
Yin, Y., Zheng, K., Dong, J., Fang, Q., Wu, S., Wang, L. y Zhang, Z. (2014). Identification of a new tospovirus causing necrotic ringspot on tomato in China. Virology Journal, 11, 213. DOI: https://doi.org/10.1186/s12985-014-0213-0