Polyclonal antiserum for the detection of Cymbidium mosaic virus and Odontoglossum ringspot virus Production
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Abstract
Orchids (family Orchidaceae) are susceptible to several biotic and abiotic factors that affect their development and quality. Among them, Cymbidium mosaic virus (CymMV) and Odontoglossum ingspot virus (ORSV) are the most frequent pathogens, with the greatest economic impact worldwide. The mechanical transmission of these viruses in addition to the predominantly agamic multiplication of orchids, make it necessary to work with virus-free plant material. The objective of this work was to develop diagnostic reagents for the detection of CymMV and ORSV. A polyclonal antiserum against CymMV + ORSV was made with a titer of 1:10000 determined by NC-ELISA. The antiserum was processed generating IgG and IgG-conjugated” anti CymMV+ORSV were obtained for use in DAS-ELISA in a dilution of 1:500. The immunodiagnostic
capacity of this technique was evaluated by analyzing samples from orchids with characteristic viral symptoms, collected from commercial nurseries in the city of Córdoba, Argentina. Viral infections were detected in 22 % of the samples analyzed and in 75 % of the nurseries surveyed. The ability of these reagents to identify CymMV, ORSV and mixed infections in orchids, will speed up analyzes and reduce their costs. This would represent an advantage for large-scale detections.
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