The role of ascorbic acid in the preservation or degradation of chlorophyll in oat leaves
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Abstract
In oat leaf segments incubated in water, chlorophyll breakdown occurs faster in darkness than under light conditions. Ascorbic acid addition to the incubation medium reversed this behavior, inducing a delay of chlorophyll breakdown in darkness but an increase of the pigment degradation in light. The accelerating effect on the chlorophyll loss in light is accompanied by an increase of malondialdehyde and membrane permeability. This effect seems to be mediated by oxygen free radicals, mainly hydroxyl radical (OH), a fact supported by the in vitro breakdown of isolated chlorophyll by Fenton's reagents and the benzoate prevention of the chlorophyll loss. Ascorbic acid in darkness prevents chlorophyll degradation connected with guaiacol peroxidase inactivation, a result also observed in the presence of the inhibitor of protein synthesis cycloheximide. Results suggest that the acceleration of chlorophyll loss in light caused by ascorbate may be due to the increase of photooxidative processes, and the effect of ascorbate in darkness may result from the inactivation of the guaiacol peroxidase activity.
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