Somatic embryogenesis in soybean (Glycine max (L.) Merrill) var. Bragg
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Abstract
The present paper describes a procedure to induce somatic embryogenesis in soybean var. Bragg. Three sources of explants were tested: cotyledons of immature seeds, cotyledonary leaves, and hypocotyl sections from 25-day-old plants. Only cotyledons of immature seeds developed embryogenic calli and somatic embryos. Initially, embryogenic calli were induced by culturing immature cotyledons in MS medium with 0 to 20 mg/l α-naphthalenacetic acid (ANA) and 0 to 40 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The best hormonal treatment was 0 mg/l ANA and 30 mg/l 2,4-D. After 30 days of subculture under the same conditions, somatic embryos were apparent, and complete plants were then obtained by culturing somatic embryos with 0.15 mg/l ANA, 0.33 mg/l kinetin, and 0.33 mg/l 6-benzylaminopurine.
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