Plant regeneration from mature embryos of maize (Zea mays L.)
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Abstract
In order to evaluate the induction of callus capable of plant regeneration, scutellum, plumule, radicle, and embryonary nodal tissues excised from mature embryos of a commercial F1 hybrid of maize were cultured in vitro. Six to ten days after culture initiation, plumule tissues turned brown and lost viability. The same response was observed 15-20 days after sowing scutellar slices as explants. Only central node and radicles were adequate tissues for callus induction under the conditions used. After 90 days in culture, viable calli were transferred to regeneration medium. Plants were regenerated in 42% of the central-node-induced calli (mean of two experiments). Radicle tissues only regenerated roots. This study reports a simple and efficient protocol to obtain plant regeneration from callus induced from the central node of maize mature embryos.
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References
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