Impact of plant regulator concentration on in vitro scion formation in three flax (Linum usitatissimum L.) explants.
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Abstract
The regeneration of shoots from cotyledons, segments of shoot, and leaf explants of Linum usitatissimum L. were evaluated in vitro on Murashige and Skoog's medium supplemented with single substances at different concentrations in a range of mg l⁻¹ (0.01; 0.1; 1.0 and 3.0) of IAA, NAA, and 2,4-D, or combinations with BAP at the same concentrations. The percentage of explants with shoots and the average number of shoots (NV) were analyzed. The optimal combination of growth substances for bud regeneration on segments of shoot explants was 1 mg l⁻¹ BAP, and 1 mg l⁻¹ BAP + 0.1 mg l⁻¹ NAA for cotyledons and leaf explants. A 100 % regeneration and NV = 7.7 from segments of shoot were induced, whereas 23 % and 33 % with NV = 3 and 5, respectively, were achieved from cotyledons and leaf explants. No differences were found in the regeneration capacity of explants from different cultivars.
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References
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