Salivary and plasmatic incorporation of fatty acids after their immediate intake in rats
Keywords:
fatty acids, saliva, plasmaAbstract
Objective: the aim of this study was to analyze salivary and plasma fatty acid (FA) levels after their immediate intake in rats.Methods: 6 adult male Wistar rats were fed on commercial diet until the 12th week of life, in which it was replaced by a laboratory diet containing corn oil (6%) as a lipid source (linoleic 18: 2 n-6 FA). At 12 and 24 hours after the diet change, rats were anesthetized and salivary secretion was induced by an intraperitoneal injection of isoproterenol / pilocarpine (5 mg/kg each) before their sacrifice. Total saliva (S) was collected for 20 min by using intraoral cotton rolls. Blood was obtained (cardiac puncture) to separate plasma (P) (centrifugation). S and P lipids were extracted for FA methylation and analysis by gas chromatography - mass spectrometry. Spearman correlation and linear regression coefficients (p≤0.05) were applied. Results: 18:2 n-6 FA was detected in S and P at 12 and 24 hours, being higher at 24 hours and in P. A higher number of FA was also found at 12 and 24 hours both in P than in S. 24/12 hour concentration ratios in S were 16:0 (1.20); 16:1 (1.65); 18:0 (0.33); 18:1 n-9 (1.55); 18:2 n-6 (1.70); 20:4 n-6 (1.20) and 22:6 n-3 (1.19) and in P: 16:0 (0.49); 16:1 (4.84); 18:0 (3.43); 18:1 n-9 (3.14); 18:2 n-6 (1.75); 20:4 n-6 (0.52) and 22:6 n-3 (0.73). A significant positive correlation between S and P 16:1 n-9 FA and a high regression between 16:0;16:1 n-9 and 18:0 salivary/plasma FA was found. Conclusion: this preliminary study showed that the 18:2 n-6 FA intake is rapidly reflected in rat S and P, with a time increase, and that there is a correlation between some FA of both fluids.
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