Salivary and plasmatic incorporation of fatty acids after their immediate intake in rats
Keywords:
fatty acids, saliva, plasmaAbstract
Objective: the aim of this study was to analyze salivary and plasma fatty acid (FA) levels after their immediate intake in rats.Methods: 6 adult male Wistar rats were fed on commercial diet until the 12th week of life, in which it was replaced by a laboratory diet containing corn oil (6%) as a lipid source (linoleic 18: 2 n-6 FA). At 12 and 24 hours after the diet change, rats were anesthetized and salivary secretion was induced by an intraperitoneal injection of isoproterenol / pilocarpine (5 mg/kg each) before their sacrifice. Total saliva (S) was collected for 20 min by using intraoral cotton rolls. Blood was obtained (cardiac puncture) to separate plasma (P) (centrifugation). S and P lipids were extracted for FA methylation and analysis by gas chromatography - mass spectrometry. Spearman correlation and linear regression coefficients (p≤0.05) were applied. Results: 18:2 n-6 FA was detected in S and P at 12 and 24 hours, being higher at 24 hours and in P. A higher number of FA was also found at 12 and 24 hours both in P than in S. 24/12 hour concentration ratios in S were 16:0 (1.20); 16:1 (1.65); 18:0 (0.33); 18:1 n-9 (1.55); 18:2 n-6 (1.70); 20:4 n-6 (1.20) and 22:6 n-3 (1.19) and in P: 16:0 (0.49); 16:1 (4.84); 18:0 (3.43); 18:1 n-9 (3.14); 18:2 n-6 (1.75); 20:4 n-6 (0.52) and 22:6 n-3 (0.73). A significant positive correlation between S and P 16:1 n-9 FA and a high regression between 16:0;16:1 n-9 and 18:0 salivary/plasma FA was found. Conclusion: this preliminary study showed that the 18:2 n-6 FA intake is rapidly reflected in rat S and P, with a time increase, and that there is a correlation between some FA of both fluids.
References
1. Van Meer G, Voelker DR, Feigenson G. Membrane lipids: where they are and how they behave. Nat Pub Group. 2008; 9:112-124.
2. Wiktorowska-Owczarek A, Berezińska M, Nowak JZ. PUFAs: structures, metabolism and functions. Adv Clin Exp Med. 2015; 24:931-41.
3. Chee B, Park B, Fitzsimmons T, Coates AM, Bartold PM. Omega-3 fatty acids as an adjunct for periodontal therapy-a review. Clin Oral Investig. 2016; 20:879-94.
4. Andreatta MM, Navarro A, Muñoz SE, Aballay L, Eynard AR. Dietary patterns and food groups are linked to the risk of urinary tract tumors in Argentina. Eur J Cancer Prev. 2010; 19:478-84.
5. Cao Y, Hou L, Wang W. Dietary total fat and fatty acids intake, serum fatty acids and risk of breast cancer: A meta-analysis of prospective cohort studies. Int J Cancer. 2016; 138:1894-904.
6. Pertiwi K, Kok DE, Wanders AJ, de Goede J, Zock PL, Geleijnse JM. Circulating n-3 fatty acids and linoleic acid
as indicators of dietary fatty acid intake in post-myocardial infarction patients. Nutr Metab Cardiovasc. 2019; 19:301-8.
7. Puy CL. The role of saliva in maintaining oral health and as an aid to diagnosis. Med Oral Patol Oral Cir Bucal. 2006; 11: 449-455.
8. Brosky ME. The role of saliva in oral health: strategies for prevention and management of xerostomia. J Support Oncol. 2007; 5:215-225.
9. Lima DP, Diniz DG, Moimaz SA, Sumida DH, Okamoto AC. Saliva: reflection of the body. Int J Infect Dis. 2010; 14:184-8. 10. Rodríguez RPCB, de Andrade Vieira W, Siqueira WL, et al. Saliva as an alternative to blood in the determination of uremic state in adult patients with chronic kidney disease: a systematic review and meta-analysis. Clin Oral Investig. 2020; 24:2203-2217.
11. Colin D. Salivary flow patterns and the health of hard and soft oral tissues. J Am Dent Assoc. 2008; 139:18-24.
12. Alam SQ, Shi YY. The effect of essential fatty acid deficiency on the fatty acid composition of different salivary glands and saliva in rats. Arch Oral Biol. 1997; 42:727-734.
13. Defagó MD, Perovic NR, Valentich MA, Repossi G, Actis AB. Omega-3 and Omega-6 salivary fatty acids as markers of dietary fat quality: A cross-sectional study in Argentina. Acta Odontol Latinoam. 2018; 31:97-103.
14. Furtado JD, Beqari J, Campos H. Comparison of the utility of total plasma fatty acids versus those in cholesteryl ester, phospholipid, and triglyceride as biomarkers of fatty acid intake. Nutr. 2019; 3: 1-17.
15. Reeves PG. Nielsen FH. Fahey GC. Jr. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993; 123: 1939-1951.
16. Folch J, Lees M, Stanley GH. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 1957; 226:497-508.
17. Infostat v.p.1. Grupo InfoStat, Facultad de Ciencias Agropecuarias, U.N.C, Argentina 2005.
18. Alam SQ, Alam BS. Effect of dietary lipids on saliva composition. J Nutr. 1982; 112: 990-996. 19. Escandriolo Nackauzi J, Repossi G, Bernal C, Actis A, Gallará R. Dietary fatty acids and the time elapsed from their intake are related to their composition in rat submandibular gland and salivary flow rates. Clin Oral Investig. 2020; 10: 1-9.
20. Onozato M, Okanishi Y, Akutsu M, Okumura I, Nemoto A, Takano K, Sakamoto T, Ichiba H, Fukushima T. Alteration in plasma docosahexaenoic acid levels following oral administration of ethyl icosapentate to rats. Pract Lab Med. 2019; 18: 1-6.
21. Childs CE, Romeu-Nadal M, Burdge GC, Calder PC. The polyunsaturated fatty acid composition of hepatic and plasma lipids differ by both sex and dietary fat intake in rats. J Nutr. 2010; 140:245-50.
22. Ranković S, Popović T, Martačić JD, Petrović S, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Glibetić M. Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes. Lipids Health Dis. 2017; 16:94.
23. Kassem AA, Abu Bakar MZ, Yong Meng G, Mustapha NM. Dietary (n-6: n-3) fatty acids alter plasma and tissue fatty acid composition in pregnant Sprague Dawley rats. Sci World J. 2012; 2012:851437.
24. Hodson L, Eyles HC, McLachlan KJ, Bell ML, Green TJ, Skeaff CM. Plasma and erythrocyte fatty acids reflect intakes of saturated and n-6 PUFA within a similar time frame. J Nutr. 2014; 144:33-41.
25. Defagó MD, Perovic NR, Valentich MA, Repossi G, Actis AB. Omega-3 and Omega-6 salivary fatty acids as markers of dietary fat quality: A cross-sectional study in Argentina. Acta Odontol Latinoam. 2018; 31:97-103.
26. Actis AB, Perovic NR, Defagó D, Beccacece C, Eynard AR. Fatty acid profile of human saliva: a possible indicator of dietary fat intake. Arch Oral Biol. 2005; 50: 1-6.
27. Marchioni DM, de Oliveira MF, Carioca AAF, Miranda AAM, Carvalho AM, Oki E, Norde MM, Rogero MM, Damasceno NRT, Fisberg RM. Plasma fatty acids: Biomarkers of dietary intake? Nutrition. 2019; 59:77-82.
28. Astorg P, Bertais S, Laporte F, Arnault N, Estaquio C y col. Plasma n-6 and n-3 polyunsaturated fatty acids as biomarkers of their dietary intakes: a cross-sectional study within a cohort of middle-aged French men and women. Eur J of Clin Nutr. 2008; 62:1155–1161.
29. Song X, Huang Y, Neuhouser ML, Tinker LF, Vitolins MZ, Prentice RL, Lampe JW. Dietary long-chain fatty acids and carbohydrate biomarker evaluation in a controlled feeding study in participants from the Women's Health Initiative cohort. Am J Clin Nutr. 2017; 105:1272-1282.
30. Furtado JD, Beqari J, Campos H. Comparison of the utility of total plasma fatty acids versus those in cholesteryl ester, phospholipid, and triglyceride as biomarkers of fatty acid intake. Nutr. 2019; 3: 1-17.
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