¿Qué son las bacterias viables no cultivables? (revisión literaria)

Autores/as

  • María Gabriela Paraje Prof. Dra. María Gabriela Paraje
  • Lucía Tamagnini

Palabras clave:

bacterias viables no cultivables, ambiente, salud.

Resumen

La viabilidad de las células bacterianas ha sido determinada, tradicionalmente, por su habilidad para crecer y formar colonias en medios de cultivo en el laboratorio o por estudios en animales. Sin embargo, un número importante de microorganismos presentes en el ambiente aún no han podido ser cultivados. Estudios recientes han demostrado la habilidad de algunas bacterias para subsistir en un estado llamado viable no cultivable (BVNC). La exposición a diferentes tipos de estrés puede inducir la formación de este estado; no obstante las células pueden retornar a un estado cultivable (“resucitar”) bajo estímulos adecuados. En esta revisión se describen las causas y regulación del estado viable no cultivable, los métodos de detección, la “resucitación” de estas células, la repercusión en comunidades microbianas (biopelículas o biofilms) y su importancia en el ambiente, la industria alimenticia y la salud pública.

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Biografía del autor/a

María Gabriela Paraje, Prof. Dra. María Gabriela Paraje

Profesora Titular Cátedra de MicrobiologíaDepartamento de FisiologíaEscuela de BiologíaFacultad de Ciencias Exactas, Físicas y Naturales  Universidad Nacional de Córdoba
Investigadora del CONICETInstituto Multidisciplinario de Biología Vegetal (IMBIV)Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET)

Citas

Amel B. K-N., Amine B.y Amina B. (2008), “Survival of Vibrio fluvialis in seawater under starvation conditions”, Microbiological Research, 163: 323-328.

Arce Miranda J.E., Sotomayor C.E., Albesa I.y Paraje M.G. (2011), “Oxidative and nitrosative stress in Staphylococcus aureus biofilm”, FEMS Microbiology Letters, 315: 23–29.

Ayrapetyan M., Williams T.C.y Oliver J.D. (2015), “Bridging the gap between viable but non-culturable and antibiotic persistent bacteria”, Trends in Microbiology, 23: 7-13.

Baffone W., Casaroli A., Citterio B., Pierfelici L., Campana R. Vittoria E., Guaglianoneb E.y Donellib G. (2006), “Campylobacter jejuni loss culturability in aqueous microcosms and ability to resuscitate in a mouse model”, International Journal of Food Microbiology, 107: 83-91.

Boaretti M., Lleó M., Bonato B., Signoretto C.y Canepari P. (2003), “Involvement of rpoS in the survival of Escherichia coli in the viable but non-culturable state”, Environmental Microbiology, 5: 986-996.

Cook K. L.y Bolster C. H. (2007), “Survival of Campylobacter jejuni and Escherichia coli in groundwater during prolonged starvation at low temperature”, Journal of Applied Microbiology, 103: 573-583.

Cunningham E., O’Byrne C.y Oliver J.D. (2009), “Effect of weak acids on Listeria monocytogenes survival: evidence for a viable but nonculturable state in response to low pH”, Food Control, 20: 1141-1144.

del Campo R., Russi P., Mara P., Mara H., Peyrou M. de León I.P. y Gaggero C. (2009), “Xanthomonas axonopodis pv. citri enters the VBNC state after copper treatment and retains its virulence”, FEMS Microbiology Letters, 298: 143-148.

Dinu L.D.y Bach S. (2011). “Induction of viable but nonculturable Escherichia coli O157:H7 in the phyllosphere of lettuce: a food safety risk factor”. Applied and Environment Microbiology, 77: 8295-8302.

Du M., Chen J., Zhang X., Li A., Li Y.y Wang Y. (2007), “Retention of virulence in a viable but nonculturable Edwardsiella tarda isolate”, Applied and Environment Microbiology, 73: 1349-1354.

Ducret A., Chabalier M.y Dunkan S. (2014), “Characterization and resuscitation of “non-culturable” cells of Legionella pneumophila”, BMC Microbiology, 14: 3.

Gengenbacher M. y Kaufmann S.H.E. (2012), “Mycobacterium tuberculosis: success through dormancy”, FEMS Microbiology Reviews, 36: 514-532.

Giao M.S. y Keevil C.W. (2014), “Listeria monocytogenes can form biofilms in tap water and enter into the viable but non cultivable state”, Microbial Ecology, 67: 603-611.

Gupta R.K.y Srivastava R. (2012), “Resuscitation promoting factors: a family of microbial proteins in survival and resuscitation of dormant Mycobacteria”, Indian Journal of Microbiology, 52: 114-121.

Kana B.D., Gordhan B.G., Downing K.J., Sung N. Vostroktunova G., Machowski E.E. Asano K., Nakatsu C.H.y Tanaka M. (2008), “The resuscitation promoting factors of Mycobacterium tuberculosis are required for virulence and resuscitation from dormancy but are collectively dispensable for growth in vitro”, Molecular Microbiology, 67: 672-684.

Kusumoto A., Asakura H.y Kawamoto K. (2012), “General stress sigma factor RpoS influences time required to enter the viable but non-culturable form”, Microbiology and Immunology, 56: 228-237.

Li L., Mendis N., Trigui H., Oliver J.D.y Faucher S.P. (2014), “The importance of the viable but non-culturable state in human bacterial pathogens”, Frontiers in Microbiology, Doi: 10.3389/fmicb. 2014.00258.

Maalej S.D., Denis M.y Dulsan S. (2004), “Temperature and growth-phase effects on Aeromonas hydrophila survival in natural seawater microcosms: role of protein synthesis and nucleic acid content on viable but temporarily nonculturable response”, Microbiology, 150: 181187.

Mishra A., Taneja N.y Sharma M. (2011), “Demostration of viable but nonculturable Vibrio cholera O1 in fresh water environment of India using ciprofloxacin DFA-DVC method”, Letters in Applied Microbiology, 53: 124-126.

Mukamolova G.V., Murzin A.G., Salina E.G., Demina G.R., Kell D.B., Kaprelyants A.S.y Young M. (2006), “Muralytic activity of Micrococcus luteus Rpf and its relationship to physiological activity in promoting bacterial growth and resuscitation”, Molecular Microbiology, 59: 84-98.

Mulcahy L.R., Isabella V.M.y Lewis K. (2013), “Pseudomonas aeruginosa biofilms in disease”, Microbial Ecology, 68: 1-12.

O’Toole G., Kaplan H.B.y Kolter R. (2000), “Biofilm formation as microbial development”, Annual Review of Microbiology, 54: 49-70.

Oliver J. D. (2005), “The viable but nonculturable state in bacteria”, Journal of Microbiology, 43: 93-100.

Oliver J.D. (2010), “Recent findings on the viable but nonculturable state in pathogenic bacteria”, FEMS Microbiology Review, 34: 415-425.

Paraje M.G. (2011), “Antimicrobial resistance in biofilms”, En: Science against microbial pathogens: communicating current research and technological advances, 736-744, FORMATEX RESEARCH CENTER, Spain.

Pasquaroli S., Zandri G. Vignaroli C., Vuotto C. Donelli G. y Biavasco, F. (2013), “Antibiotic pressure can induce the viable but non-culturable state in Staphylococcus aureus growing in biofilms”, Journal of Antimicrobial Chemoteraphy, 68: 1812-1817.

Pinto D., Santos M.A.y Chambel L. (2013), “Thirty years of viable but nonculturable state rearch: unsolved molecular mechanisms”, Critical Reviews in Microbiology doi:10.3109/1040841X.2013.794127.

Polifroni R., Etcheverría A.I., Padola N.L.y Parma A.E. (2009), “Escherichia coli verotoxigénico (VTEC). Características de virulencia y persistencia en el medio ambiente”, InVet, 11: 65-70

Puspita I.D., Uehara M., Katayama T., Kikuchi Y., Kitagawa W., Kamagata Y., Asano K., Nakatsu CH. y Tanaka M. (2013), “Resuscitation promoting factor (Rpf) from Tomitella biformata AHU 1821(T) promotes growth and resuscitates non-dividing cells”, Microbes and Environment, 28: 58-64.

Ramamurthy T., Ghosh A., Pazhani G.P. y Shinoda S. (2014), “Current perspectives on viable but non-culturable (VBNC) pathogenic bacteria”, Frontiers in Microbiology, Doi: 10.3389/fpubh.2014.00103.

Reisbrodt R., Rienaecker I., Romanova J.M., Freestone P.P.E., Haoigh R.D., Lyte M., Tschäpe H. y Williams P.H. (2002), “Resuscitation of Salmonella enterica serovar Typhimurium and enterohemorragic Escherichia coli from the viable but nonculturable state by heat-stable enterobacterial autoinducer”, Applied and Environment Microbiology, 68: 4788-4794.

Sardessai Y. N. (2005), “Viable but non-culturable bacteria: their impact on public health”, Current Science, 89: 1650.

Su X., Chen X., Hu J., Shen C.y Ding L. (2013), “Exploring the potential environmental functions of viable but non-culturable bacteria”, World Journal of Microbiology and Biotechnology, 29: 2213-2218.

Wong H.C.y Wang P. (2004), “Induction of viable but nonculturable state in Vibrio parahaemolyticus and its susceptibility to environmental stresses”, Journal of Applied Microbiology, 96: 359-366.

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Publicado

2015-09-14

Cómo citar

Paraje, M. G., & Tamagnini, L. (2015). ¿Qué son las bacterias viables no cultivables? (revisión literaria). Revista De La Facultad De Ciencias Exactas, Físicas Y Naturales, 2(2), 99–102. Recuperado a partir de https://revistas.unc.edu.ar/index.php/FCEFyN/article/view/10745

Número

Sección

Ciencias Naturales