Formation of plaque in vitro by Gandida Albicans and Chlorhexidina a ction

Abstract

The :Presence of Candida Albicans in bacteria! plaques taken from the vestibular and lingual faces of the · upper aud ·lower molars was investigated in a group of students from the School of Dentistry ranging from 18 to 24 years of age. Candida Albicans was found in the 21,14% of the cases studied.

The ez.:perience that is composed of two parts was perforned starting from thease strains: the formation of plaque "in vitro" was investigated during the fust part of the experienée. It w'as cultivated in a base rned~um adding carbohydrates, namely, glucose, saccharose, lactose, starch and rrialtose obtaining plaques formation with different gradations in quantity and adherence according to sugars. Starting from these results the second part of the experience was initiated. It consisted in the aggregate of different concentrations of chlorhexidine to the culture medium to be used .. Inbibition of plaque in vitro was proved in concentrations 6f up to 0,15% in sorne strains. From tbis we could determine that: 1) tbis' microorganism is_ found in bacteria] plaques in a relevant percentage of cases (21,14% ). 2) Gandida Albicans forms plaque in vitro starting from carbohydrates; 3) antiplaque substances like Ghiorhexidine can inhibit thet Cj.eveloprnent of Gandida Albicans in concentrations superior to 0,2%.