Métodos cromatográficos otimizado para a identificação e quantificação de terpenos em óleo de Cannabis sativa para uso médico
DOI:
https://doi.org/10.31053/1853.0605.v80.n2.39593Palavras-chave:
terpenos, cromatografia de gás, calidad, controle de qualidadeResumo
Introdução: A Cannabis sativa é uma espécie vegetal com um grande número de princípios ativos, por isso a lista de seus usos terapêuticos está se expandindo. A este respeito, há amplas evidências do uso medicinal potencial dos terpenos, bem como de sua sinergia com os canabinoides (efeito de comitiva). Assim, à medida que mais países contemplam a legalização e autorização da canábis medicinal, o número de laboratórios de extração e teste de canábis está aumentando para atender à demanda, exigindo ferramentas analíticas apropriadas. Metodologia: Em resposta a numerosas consultas de médicos, laboratórios de análises e usuários, o laboratório de cromatografia PROBIEN selecionou dois métodos para a análise de terpenos em óleo de canábis pela técnica de cromatografia de gás (GC-FID). Os métodos são descritos usando as colunas HP-5 e Innowax. O método padrão externo foi utilizado para a determinação quantitativa de β-Pinene, Myrcene, p-Cymene, Limonene, Linalool, α-Terpineol, Nerol e Geraniol. Resultados: Foram observados bons picos de separação e reprodutibilidade, adequados para a identificação e quantificação dos principais terpenos em extratos de Canábis. A relação área/concentração foi linear na faixa de 0,0005 a 2,0 mg/ml. Conclusão principal: Os métodos descritos permitem a identificação e quantificação dos principais terpenos no óleo de Canábis para um controle de qualidade certo.
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