DETERMINATION OF MERCURY IN VEGETAL TISSUES

BY MICROPIXE: APPLICATION TO THE STUDY OF

HYPERACCUMULATION BY SPIRODELA INTERMEDIA

(LEMNACEAE)

DETERMINACIÓN DEL MERCURIO EN TEJIDOS VEGETALES POR MICROPIXE:

APLICACIÓN AL ESTUDIO DE LA HIPERACUMULACIÓN POR SPIRODELA

INTERMEDIA (LEMNACEAE)

Emmanuel M. de la Fournière1,2, Nahuel A. Vega1,2, Nahuel A. Müller1, Ramón A. Pizarro3 and Mario E. Debray1,2*

SUMMARY

1.Gerencia Investigación y Aplicaciones, Comisión Nacional de Energía Atómica, CAC, Av. Gral. Paz 1499, B1650KNA San Martín, Prov. de Buenos Aires, Argentina.

2.ECyT, Universidad Nacional de Gral. San Martín, M. de Irigoyen 3100, San Martín, Buenos Aires, Argentina.

3.División Radiomicrobiología

del Dto. Radiobiología, Comisión Nacional de Energía Atómica, CAC, Av. Gral. Paz 1499, B1650KNA San Martín, Prov. de Buenos Aires, Argentina.

*debray@tandar.cnea.gov.ar

Citar este artículo

DE LA FOURNIÈRE, E. M., N. A. VEGA, N. A. MÜLLER, R. A. PIZARRO & M. E. DEBRAY. 2019. Determination of mercury in vegetal tissues by microPIXE: Application to the study of hyperaccumulation by Spirodela intermedia (Lemnaceae). Bol. Soc. Argent. Bot. 54: 263-275.

DOI: http://dx.doi. org/10.31055/1851.2372.v54. n2.24373

Recibido: 24 Octubre 2018

Aceptado: 11 Febrero 2019

Publicado: 30 Junio 2019

Editor: Omar Varela

ISSN versión impresa 0373-580X ISSN versión on-line 1851-2372

Background and aims:Aqueous mercury (II), Hg2+, is still nowadays a hazardous pollutant with a large dispersion. Phytoremediation strategies are an environmental friendly and low-cost alternative. In order to improve these processes, Spirodela intermedia, an autochthonous floating macrophyte, was used to remove Hg2+ from mineral water under laboratory conditions, studying the in vivo distribution of mercury and other elements by nuclear microprobe scanning mapping.

M&M: Exposures (1 and 10 mg.L-1 Hg2+ concentrations) were performed during at least 2 weeks. All the parameters from the bioremediation process as uptake rate, bioconcentration factors (BCFs) of mercury in roots and leaves and translocation factors (TFs), were achieved from microPIXE quantifications at BuenosAires Tandar accelerator.

Results: For 1 and 10 mg.L-1 concentrations, S. intermedia can be considered as a hyperaccumulator. The highest BCFs (> 1000 in roots and > 200 in leaves) were obtained for 1 mg.L-1 of Hg2+ at 96 h. In all cases TFs < 1 were measured, indicating that Hg2+ translocation is not taking place. High resolution spatial 2D maps of the in vivo distribution for different exposure conditions were established. It was observed that Hg2+ distribution in leaves is more heterogeneous than in roots. An important finding was the detection of Hg in chlorenchyma where its effects are more toxic. Correlation between mercury and calcium distribution and its relationship with physiological responses to intoxication have been examined.

Conclusions: Phytoremediation of Hg2+ by S. intermedia is a convenient alternative. Since the protocol was performed using a real water, it becomes an advisable tool at higher scale.

KEY WORDS

Spirodela intermedia, mercury, hyperaccumulation, microPIXE.

RESUMEN

Introducción y objetivos: El mercurio (II) acuoso, Hg2+, es todavía un contaminante peligroso ampliamente distribuido. Las estrategias de fitorremediación son ambientalmente amigables y de bajo costo. Con el fin de optimizar estos procesos, se utilizó Spirodela intermedia, una macrófita acuática autóctona, para remover Hg2+ en agua mineral, en condiciones de laboratorio, estudiando la distribución in vivo de mercurio y otros elementos por mapeo barriendo con una microsonda nuclear.

M&M: Las exposiciones (concentraciones de Hg2+ de 1 y 10 mg.L-1) duraron al menos 2 semanas. Todos los parámetros del proceso como tasa de captación, factores de bioconcentración (BCFs) de mercurio en raíces y frondes y factores de translocación (TFs) fueron calculados a partir de cuantificaciones de microPIXE con el acelerador Tandar de Buenos Aires.

Resultados: S. intermedia puede ser considerado un hiperacumulador. Los más altos BCFs (> 1000 en raíces y > 200 en frondes) correspondieron a 1 mg.L-1 a las 96 hs. En todos los casos, se constató que TFs < 1, indicando que no ocurre translocación de Hg2+. Se obtuvieron mapas 2D de alta resolución espacial de la distribución elemental in vivo para las diferentes condiciones. Se observó que la distribución de mercurio en frondes es más heterogénea que en raíces. Fue importante la detección de Hg en clorénquima donde sus efectos son más tóxicos. Se analizó una correlación entre la distribución de mercurio y calcio y la relación con respuestas fisiológicas.

Conclusiones: La fitorremediación de Hg2+ con S. intermedia es una alternativa conveniente. Por haberse realizado en agua real, el protocolo es escalable.

PALABRAS CLAVE

Spirodela intermedia, mercurio, hiperacumulación, microPIXE.

263

Bol. Soc. Argent. Bot. 54 (2) 2019

INTRODUCTION

The impact of mercury compounds in the natural environment represents even now a very important matter (Nriagu, 1979). 1 µg.L-1 has been established as maximum level for human water consumption in Argentina (Código Alimentario Argentino, 2012). Particularly, in Argentina, mercury compounds were employed as pesticides in tobacco plantations (García et al., 2003). In last years, the presence of Hg contamination in groundwater and surface water has been a very topical issue due to the spillage of cyanide solutions produced by the activity of the Veladero mine in the province of San Juan, Argentina (Ford et al., 2015). Methylmercury, the most toxic mercury species, is produced by biotic and abiotic Hg2+ methylation (Celo et al., 2006; King et al., 2001; Yin et al., 2012). To avoid this serious process, aqueous Hg2+ must be treated.

The treatment of aqueous mercury is problematic. Chemical procedures present several drawbacks, such as solid wastes disposal (Serpone et al., 1988). More recent removal strategies, known as advanced oxidation technologies (AOT’s), improve highly the efficiency of inorganic and organomercurial compounds removal. AOT experiments are generally performed in pure water (de la Fournière et al., 2007 and references therein) but, in real waters, removal rates are remarkably lower.

Bioremediation is more environmentally friendly and is largely cheaper than chemical treatments. Phytoremediation by aquatic macrophytes has been especially widely reported motivated by their hyperaccumulating capacities of the soluble and bioavailable contaminants, chiefly metals and metalloids, from water (Miretzky et al., 2004; Mishra et al., 2009; Rahman & Hasegawa, 2011). Floating macrophytes accumulate mainly in their roots (Vardanyan & Ingole, 2006). Autochthonous organisms should be selected to avoid any ecosystem unbalance. Consequently, in this work, remediation experiments using duckweed Spirodela intermedia W. Koch (Lemnaceae) have been carried out. This species is reported in lentic water bodies of Argentina and other regions of Central and South America (Feijoo & Lombardo, 2007; Basílico et al., 2013). In this paper, removal of HgCl2 dissolved in mineral water is studied, focusing on the in vivo mercury distribution. Absorption process modeling and correlation between mercury and calcium

uptake, possibly involved in a mechanism of resistance, have been also investigated.

MATERIAL AND METHODS

In this study, the spatial distribution of Hg was analyzed by microPIXE scanning mapping, namely PIXE with highly-focused ion beams with micrometric dimensions (Barnabas et al., 1999). The sensitivity of this technique allows simultaneous mapping of main, minor and trace elements. Combined with STIM (Scanning Transmission Ion Microscopy) and micro-RBS (Rutherford Back Scattering) provides the quantitative determination of trace elements concentration with high sensitivity (µg.g-1 range) and multi-elemental distribution maps with high spatial resolution (micrometric range) of the irradiated region conserving the structure of the sample (Lefevre et al., 1991; Witkowski et al., 1997, Barnabas et al., 1999). Usually, the typical detection limits for most elements are in the range of 1−10 μg.g-1.

The heavy ion beam microprobe in Buenos Aires Focusing is performed with the aid of the Tandar

Laboratory heavy-ion microprobe onto a spot of about 3-5 µm in diameter on Hg contaminated transversal sections of roots and aerial parts and of non-contaminated controls. The samples were irradiated normal to the incident particle beam and measured at 135o with respect to the beam direction, to minimize the X-ray background. The largest area that can be scanned under these conditions is about 1×1 mm2 which it is large enough to hold several cross sections roots of Spirodela intermedia. The X-rays belonging from the sample were measured with a 80 mm² high resolution silicon drift detector X-ray detector (http://www.ketek.net/). A sheet of Kapton 50 µm thick was placed in front the detector to shield it against backscattered ions and to attenuate X-rays from the light elements minimizing the pile-up in the X-ray spectrum. This sheet has not effect on the transmission at the energy of the mercury Lα line. More details of the experimental setup can be found in reference (Stoliar et al., 2004).

Sample collection and preparation

S. intermedia were collected from a natural

264

E. M. de la Fournière et al. - Hyperaccumulation of Hg by S. intermedia evaluated by microPIXE

wetland, carefully washed with Milli-Q water and placed in glass bottles filled (6 plants per bottle) illuminated with fluorescent light (Basílico et al., 2013) in a 16:8 h (light:dark) photoperiod. 250 mL of commercial mineral water was employed, spiked or not with Hg2+, without any nutrient addition since we were focused on groundwaters remediation. Characterization of the mineral water is shown in Table 1. Initial concentration of Hg2+ was 1 and 10 mg.L-1. Exposure time was from hours to weeks (12, 24, 48, 72, 96, 168 and 336 h).

In order to analyse mercury content in roots and leaves, at least three plants of each condition were withdrawn and immediately frozen. Transversal cross-sections ~ 10 µm thick of roots (Fig. 1) and leaves were obtained using a cryo-microtome at -20 °C to avoid ions migration which could alter in vivo distribution. Next, histological cross-sections were transferred to ultrapure polypropylene backings with acrylic-glass target frames and freeze-dried (with no further processing) and finally irradiated with the microbeam (Llabador & Moretto, 1996).

Table 1. Chemical characterization of mineral water used.

pH

EC

Ca2+

Mg2+

Na+

F-

K+

Cl-

HCO3-

SO42-

TDS

mS.cm-1

mg.L-1

mg.L-1

mg.L-1

mg.L-1

mg.L-1

mg.L-1

mg.L-1

mg.L-1

mg.L-1

 

 

 

 

 

 

 

 

 

 

 

 

8.1±0.2

230±21

30±2

3.0±0.2

10±0.6

1.14±0.36

4±0.4

4±0.3

79±5

44±3

176±12

 

 

 

 

 

 

 

 

 

 

 

Abbreviations: EC: Electrical conductivity. DS: Total dissolved solids.

For each section, a ‘‘twin’’ contiguous section was stained to obtain an ‘‘as-close-as-possible’’ optical image of the freeze-dried section, to distinguish details of the histology which are not readily observable in the irradiated non-stained section, and correlate them with the distribution of the different elements.

Chemicals

Stock solutions were prepared using HgCl2 (Merck, Darmstadt, Germany) of the highest purity with Milli-Q water (resistivity = 18 MΩ.cm). As sample mounting was used a thin 4 µm thick Prolene film (Fluxana GmbH & Co., Bedburg- Hau, Germany, www.fluxana.com). This film was selected because microPIXE analysis of the Prolene gave a remarkably clean spectrum, showing that it contains no contaminants above the minimum detectable limit (MDL) (Southworth-Daviesa et al., 2007). The polypropylene was mounted on an aluminium support with a 10 mm diameter hole, which is held in a “holder-ladder” capable of holding up to three samples, inside the vacuum chamber.

MicroPIXE analysis

The aim this work was to investigate the distribution of Hg in roots cross-sections of the floating macrophyte Spirodela intermedia

by microPIXE (micro-Particle Induced X-ray Emission) spectrometry (Mesjasz-Przybyłowicz

&Przybyłowicz, 2002; Lyubenova et al., 2007; Vogel-Mikuš et al., 2007; Cestone et al., 2012; Wang et al., 2013; Módenes et al., 2013) to permit a better understanding of the uptake, localization, accumulation and translocation of the metal in this

Fig. 1. Typical root cross-section OM photograph of

S.intermedia. Scale= 20 µm.

265

Bol. Soc. Argent. Bot. 54 (2) 2019

plant, as well as to know and quantify the ability of this particular plant species to hyperaccumulate and tolerate the concentration of metals in their roots. This knowledge is critical to examine phytoextraction strategies through phytoaccumulation in aquatic contaminated areas.

There are several analytical tools ranging from those which allow quantify from “bulk” analysis the concentrations of metals in plants to those that have the ability to quantify and expose the elemental distribution of metals in plant tissues (IAEA, 1980; Lefevre et al., 1991; Witkowski et al., 1997; Barnabas et al., 1999; Malan et al., 2012; Mendes Godinho et al., 2013).

Of the various nuclear analytical methods using ion beams, PIXE is the more often used technique. PIXE is a high sensitivity, multi-elemental analysis technique, based on the high cross-section MeV-ion ionization of inner-shell vacancies (mainly in the K-and L-shell) and detection of subsequent emission of characteristics X-rays which yields information on the concentration of the elements present in the samples (Lefevre et al, 1991; Witkowski et al., 1997).

Thin samples of 10 µm thickness are easily broken during handling and only cuts which kept cell integrity under test by optical microscope, were irradiated. The

samples were mounted on a manual xyz translator (2 µm resolution step) in the irradiation chamber. The rough positioning of the samples was achieved using an optical digital microscopy.

The samples were irradiated with a 50 MeV energy 16O5+ ion beam focused to a < 5 µm spot size scanning the beam over a 256×256 pixel matrix. Data acquisition was carried out by scanning the beam over a given sample and saving the data at each pixel along with the simultaneous X and Y coordinates of the beam spot. These matrix data (X-ray energy, X-Y coordinates) allow us to obtain the full energy X-ray spectrum and the Hg and other elements distributions by windowing on interest X-ray lines in the PIXE spectrum. Fig. 2 shows such a spectrum for a root tissue section.

The dry mass was estimated using STIM with a 16O5+ ion beam at 50 MeV with an intensity of about 103 ions per second. Since the samples are completely traversed by the 16O beam, it is possible to determine the effective density of the sample using the energy loss contrast STIM method (Lefevre et al., 1987). Considering that the dried plant tissue samples were sliced on the cryo-microtome to a thickness of 10±0.5 µm, the STIM measurement gives 0.36±0.09 g.cm-3 as equivalent bulk tissue density. This great uncertainty

Fig. 2. MicroPIXE spectrum of an irradiated root cross-section of Spirodela intermedia exposed to 10 mg.L-1 Hg2+ solution during two weeks.

266

E. M. de la Fournière et al. - Hyperaccumulation of Hg by S. intermedia evaluated by microPIXE

in the result is intrinsic to the STIM method since the heterogeneous morphology of the sample results in an uneven area density after freeze-drying (Vogel-Mikuš et al., 2009). This value doubles the mean root tissue density ~0.15 g.cm-3 determined from morphological parameters of floating macrophyte Lemna minor (Cedergreen & Vinbӕk Madsen, 2002). However, this result is acceptable if one considers the great variability of the root density determined for different plant species. Birouste et al. (2014) measured root density variations between 0.152 and 0.683 g.cm-3 using the Archimedes’ method. With this equivalent tissue density the Hg content determined by microPIXE was normalized to express its concentration in term of μg of Hg per g of dry sample.

Data analysis

The resulting spectra were analysed off-line with the OMDAQ-2007 computer code by calculating the peak area using the Gaussian fit or directly the sum of the counts per channel under the peak. This is possible due to the cleaning of the spectrum above 9 keV

(almost free background region - excellent peak-to- background ratio) where only the X-ray peaks of the Hg lines Lα and Lβ are present and are fully resolved. By gating on the Hg Lα line, we constructed a two- dimensional Hg map which shows where and how the mercury penetrated and distributed in the transversal section of the roots.

Likewise two-dimensional maps of elemental concentration for any other element present in the X-ray spectrum can be obtained by gating the corresponding X-ray lines.

The in vivo microPIXE distribution maps of Hg (gate on Hg L lines), K and Ca in the roots of S. intermedia are shown in Fig. 3. The microPIXE images of the major elements K and Ca were chosen to indicate the structure of the root cross-section. The distribution of mercury in roots (Fig. 3) after 24 hours exposure to Hg2+ (10 mg.L-1) seems to be quite homogeneous during the uptake process if compared with in vivo distribution of mercury obtained by Lomonte et al. (2014) in roots of Chrysopogon zizanioide.

Fig. 3. 2D X-rays maps corresponding to Ca, Hg, Cl and K distribution from the root cross-section of S. intermedia exposed to Hg2+ (10 mg.L-1) during 24 hours. MicroPIXE conditions: 50 MeV 16O5+ beam, scan size 200200 m2, spot size 33 m2. The pixels change color from blue to red with the increase of the elemental concentration (see color scale included in the figure). Scale= 50 µm.

267

Bol. Soc. Argent. Bot. 54 (2) 2019

RESULTS

Uptake of mercury

Fig. 4 shows the temporal evolution of the uptake of Hg2+ for the concentrations of 1 and

10mg.L-1. It can be seen that Hg2+ is rapidly concentrated in the roots of S. intermedia. When exposed to 10 mg.L-1 of Hg2+ solution, in the first 12 h the plant content is about 2 mg.g-1 (see Fig. 4). Over the next 84 hours increases nearly threefold and then drastically decreases indicating that, for this content, mercury becomes highly toxic for the plant. For an external concentration of 1 mg.L-1, the maximum uptake of mercury is higher than that found for plants placed in a 10 mg.L-1 medium and a saturation zone is observed from 96 h, suggesting that this mercury level is still tolerated.

Various models can be used to analyse the kinetics of sorption process. The simplest kinetic model which describes the process of sorption, is the pseudo-first order rate equation suggested by Lagergren (Yuh-Shan, 2004). This model has been most widely used for the adsorption of aqueous phase pollutants such as metal ions. Mercury net-sorption kinetics can also be

modelled by this pseudo first-order equation based on solid capacity:

(1)

where, and (mg.g-1) are the mg of solute absorbed per g of sorbent at any time t and at equilibrium respectively, (mg.g-1.h-1) is the uptake rate, (h-1) is the pseudo first-order rate constant of sorption and k.qe (mg.g-1.h-1) is the initial sorption rate. Integrating this equation for the initial conditions q = 0 for t = 0 gives:

(2)

The kinetic parameters (Table 2) were obtained by fitting equation (2) to the experimental results. The curves predicted by this model are presented in Fig. 4. The correlation coefficient indicates that the least-squares fits are similar for both Hg concentrations.

As shown in Fig. 4, for an exposure to 10 mg.L-

1of Hg2+ solution (solid line), the plant rapidly increases his concentration during the first 2 days

(in just 42 hours it reaches 63% of the maximum cumulative value) and saturates to a nearly constant value of 6.2 mg.g-1 in approximately 100 hours of

Fig. 4. Hg uptake temporal profiles in roots of S. intermedia exposed to aqueous Hg2+. The dotted and solid lines correspond respectively to the adjustments [Eq. (2)] of the experimental values of the uptake of the plants exposed to solutions with concentrations of 1 and 10 mg.L-1 of Hg. Dashed line indicates mercury concentration decay (for an external 10 mg.L-1 concentration) due to plant poisoning (intoxication).

268

E. M. de la Fournière et al. - Hyperaccumulation of Hg by S. intermedia evaluated by microPIXE

Table 2. Rate constants for the pseudo-first order equation of Lagergren fitted for removing Hg2+ by Spirodela intermedia. C0 is the Hg2+ concentration in solution. S2 is the standard deviation and R2 the correlation coefficient of the fitting functions.

C

0

q

e

k ×10-2

q

.k

S2

R2

 

 

(h-1)

e

 

(mg.L-1)

(mg.g-1)

(mg.g-1.h-1)

 

 

10

6.23

2.37

0.148

0.320

0.99

 

 

 

 

 

 

1

1.06

1.6

0.017

0.122

0.97

 

 

 

 

 

 

 

 

 

exposure. However from that moment, it begins to release Hg to the aqueous medium due to the loss of its biological functions by poisoning (dashed line).

For a concentration of 1 mg.L-1, the saturation zone is not followed by decay (dotted line) and the plant reaches the 63% of maximum value after approximately 63 h.

These results suggest that in remediation applications with either of both concentrations, the plants should be eliminated immediately after the fourth day. For 10 mg.L-1 exposure, the elimination is more important because, in addition to reaching saturation, the plants start to return the captured mercury.

A possible correlation between the increase of Ca2+ concentration and the uptake of Hg2+ by roots has been evaluated. As shown in Fig. 5 and the value of the determination coefficient R2, an exponential regression correlates quite well calcium and mercury concentrations normalized to their respective concentrations at 96 hours ([Ca2+]/

[Ca2+]96h).

The fitting function:

(3)

where: a = 7.5x10-3, b = 4.5919 and c = 0.278, (a+c≈c) is the Ca2+ value of the uncontaminated

Fig. 5. Relationship between the normalized concentrations calcium [Ca2+]/[Ca2+]96h and mercury [Hg2+]/ [Hg2+]96h in roots of S. intermedia exposed to 10 mg.L-1 of Hg2+. Time interval: 096 h. Solid line: regression corresponding to Eq. (3).

269

Bol. Soc. Argent. Bot. 54 (2) 2019

root (t = 0h) and b represents the growth rate

of [Ca2+]/[Ca2+]96h fraction when the [Hg2+]/ [Hg2+]96h normalized concentration is increasing. In

accordance with this fit equation, in the analyzed samples of roots of S. intermedia exposed to 10

mg.L-1 of Hg2+, whenever the [Hg2+]/[Hg2+]96h increases a fraction 0.152, the Ca2+ uptake will

increase in such way that its [Ca2+]/[Ca2+]96h shall be doubled (regardless of the initial value at t = 0 h).

A similar analysis on the concentration of

1mg.L-1 does not allow establishing a clear relationship between the concentrations of Ca2+ and Hg2+.

It is reported that calcium is involved to counteract metal stress in hyperaccumulator plants (Tian et al., 2011). Another mechanism to avoid metal toxicity is thiol biosynthesis such as phytochelatins (PCs). In this sense, it is known that PCs are present in Spirodela genus (Pandey et al., 1999). In another hand, Ca2+ increases the expression of PC synthase gene under Cd2+ stress in Lactuca sativa (He et al., 2005). It is therefore possible that Ca2+, in presence of Hg2+, triggers PC synthesis in S. intermedia.

Translocation

Mercury in leaves has been also detected and quantified in order to evaluate possible metabolism damage during phytoremediation process.As expected, no mercury was present in leaves of controls.

Translocation factor (TF), the ratio of metal content in the leaves to those in the roots (Mattina et al., 2003), was calculated from experimental data:

TF = [Hg]leaves/ [Hg]roots

(5)

where [Hg]leaves and [Hg]roots are the in vivo concentrations of mercury in leaves and roots

respectively.

It is important to mention that S. intermedia can absorb aqueous metals through the whole plant (Porath & Pollock, 1982). Thus, in this case, it is not strictly a translocation but, anyway, TFs give valuable information when studying metabolic damage in leaves. The measured values are listed in Table 3.

In all cases, TF < 1, indicating that there is no translocation.

Bioconcentration factors in roots and leaves From experimental and fitted data shown in Fig.

4, bioconcentration factor (BCF) of mercury in roots and leaves, defined in equations (6) and (7) was calculated for the maximum uptake reached for all the initial concentrations (96 h).

BCFroots = [Hg]roots/ [Hg]water

= TF. BCFroots

(6)

BCFleaves = [Hg]leaves/ [Hg]water

(7)

Measured values are listed in Table 4.

Table 3. Translocation factor (TF) of mercury in S. intermedia calculated from experimental data for

different conditions.

[Hg2+ ] (mg.L-1)

10

10

10

1

0

 

 

 

 

Time exposure (h)

48

72

96

96

 

 

 

 

 

TF

0.21±0.02

0.22±0.02

0.33±0.03

0.19±0.02

 

 

 

 

 

Table 4. Bioconcentration factors (BCF) of mercury, in roots and leaves of S. intermedia, calculated from

experimental (mv) and fitting (fit) data, respectively.

[Hg2+]0(mg.L-1)

BCFroots(mv)

BCFroots(fit)

BCFleaves(mv)

1

1294±109

1060±104

246±23

 

 

 

 

10

409±39

623±71

189±19

 

 

 

 

270

E. M. de la Fournière et al. - Hyperaccumulation of Hg by S. intermedia evaluated by microPIXE

DISCUSSION

As can be seen in all cases aqueous Hg2+ solutions, BCFs > 100 are obtained. Thus, S. intermedia can be considered, at least in this range of mercury concentration, as a hyperaccumulator (Rascio & Navari-Izzo, 2011). It is pertinent to mention that others methods such as membrane filtration, precipitation with chemicals, ion exchange, reduction, and adsorption are much less efficient and wasteful for concentrations lower than 100 mg L-1 (Manohar et al., 2002).

The values of BCF are in concordance with those obtained by Srivastav et al. (1994) using the same macrophyte for the removal of Cr (600 ≤ BCF ≤ 711) and Ni (562 ≤ BCF ≤ 713) in the range of 1−8 mg.L-1 in tap water.

Elemental distribution patterns in leaves of a plant exposed to 10 mg.L-1 during 72 hours is displayed in Fig. 6. Ca and Cl distribution indicate the structure of a leaf cross-section. If mercury is found throughout the leaf, its distribution is heterogeneous. For example, the concentration of

Hg was higher near the lower surface (4.10 mg.g- 1) than in chlorenchyma (2.23 mg.g-1). This is a coherent result, considering that the lower surface is in contact with water.

An important aspect of this study was the localisation of mercury within the chlorenchyma. It is interesting to note that the highest concentrations of Mn in the chlorenchyma (Mn X-rays map) would correspond to the oxygen evolving Mn4CaO5 cluster (OEC) in photosystem II (Rossini & Knapp, 2017) and mercury is detected at this level.

In another hand, it is well known the toxicity of mercury on photosystem II (Deng et al., 2013). As a result, plants exposed to Hg2+ (10 mg.L-1) turned brown (Fig. 7b). The control of viability shows as expected that plants remain green when exposed to the same water without mercury (Fig. 7a).

The brown colour suggests that the replacement of magnesium from chlorophyll by mercury is not taking place since, heavy metal chlorophylls are reported to be more stable and, even dead plants remain green (Kupper et al., 1996).

Fig. 6. 2D X-rays maps corresponding to Ca, Hg, Mn and Cl distribution from the top cross-section of a leaf of S. intermedia exposed to Hg2+ (10 mg.L-1) during 72 hours. MicroPIXE conditions: scan size 300300 m2, spot size ~ 33 m2. Same color scale as Fig. 3. ls: lower surface; ch: chlorenchyma; us: upper surface. Scale= 50 µm.

271

Bol. Soc. Argent. Bot. 54 (2) 2019

Fig. 7. Spirodela intermedia after a week of exposure. A: Hg2+-free control of viability. B: Plants treated with

aqueous Hg2+(10 mg.L-1). Scales= A-B: 4 mm.

CONCLUSIONS

Phytoremediation of aqueous Hg2+ by the floating autochthonous macrophyte Spirodela intermedia is a convenient alternative. For an initial concentration of Hg2+ ranging 1−10 mg.L-1, plants behave as hyperaccumulators and reach the maximal bioconcentration in only 96 h. Exposed to an initial concentration 10 mg.L-1, plants must be collected at the fourth day to avoid Hg leakage due to intoxication.

Since the experiments were performed using mineral water, the use of S. intermedia becomes an advisable procedure of removing aqueous Hg2+ at pilot and field scale.

Low mass solid wastes are produced on account of a high Hg/biomass proportion.

It was found that Hg2+ distribution in leaves is more heterogeneous than in roots. The simultaneous mapping of various elements can be used to explore the physiological mechanisms that allow these plants to accumulate and in some cases hyperaccumulate Hg2+.

The temporal evolution of Ca2+ uptake as a mechanism to reduce the toxic effect of the incorporation of Hg2+ should be studied for concentrations below than 10 mg.L-1 in order to analyze the behaviour of this uptake for longer survival times.

ACKNOWLEDGEMENTS

To Dra. M.C. Matulewicz, Dto. de Química Orgánica-CIHIDECAR-(CONICET-UBA), FCEyN, for lyophilization of samples. E. de la Fournière thanks CONICET for a postdoctoral fellowship.

AUTHOR CONTRIBUTIONS

EMF conceived, designed and carried out all the experiments, performed data analysis and wrote the paper. NAV contributed to microPIXE irradiations especially monitoring acquisition process. NAM contributed substantially to the microbeam line maintenance, software update installation and image processing. RAP supervised biological protocol design. MED conceived and designed microPIXE protocols from sample preparation to irradiation conditions, performed data analysis and wrote as well this work. MED and RAP were director and co-director, respectively, of EMF.

BIBLIOGRAPHY

BARNABAS, A. D., W.J. PRZYBYŁOWICZ, J.

MESJASZ-PRZYBYŁOWICZ, C. A. PINEDA. 1999. Nuclear microprobe studies of elemental

272

E. M. de la Fournière et al. - Hyperaccumulation of Hg by S. intermedia evaluated by microPIXE

distribution in the seagrass Thalassodendron ciliatum. Nucl. Instr. Meth. B 158: 323-328. https://doi.org/10.1016/S0168-583X(99)00366-3

BASÍLICO, G., L. DE CABO, A. FAGGI. 2013. Impacts of composite wastewater on a Pampean stream (Argentina) and phytoremediation alternative with Spirodela intermedia Koch (Lemnaceae) growing in batch reactors. J. Environ. Manage. 115: 53-59. https://doi.org/10.1016/j.jenvman.2012.11.028

BIROUSTE, M., E. ZAMORA-LEDEZMA, C. BOSSARD, I. M. PÉREZ-RAMOS, C. ROUMET. 2014. Measurement of fine root tissue density: A comparison of three methods reveals the potential of root dry matter content. Plant Soil 374: 299-313. https://doi.org/10.1007/s11104-013-1874-y

CEDERGREEN, N. & T. VINBÆK MADSEN. 2002. Nitrogen uptake by the floating macrophyte Lemna minor. New Phytol. 155: 285-292. https://doi.org/10.1046/j.1469-8137.2002.00463.x

CELO, V., D. R. S. LEAN, S. L. SCOTT. 2006. Abiotic methylation of mercury in the aquatic environment. Sci.Total Environ. 368: 126-137. https://doi.org/10.1016/j.scitotenv.2005.09.043

CESTONE, B., K. VOGEL-MIKUŠ, M. F. QUARTACCI, N. RASCIO, P. PONGRAC, P. PELICON, P. VAVPETIČ, N. GRLJ, L.JEROMEL, P. KUMP, M. NEČEMER, M. REGVAR, F. NAVARI-IZZO. 2012. Use of micro-PIXE to determine spatial distributions of copper in Brassica carinata plants exposed to CuSO4 or CuEDDS, Sci. Total Environ. 427-428: 339-346. https://doi.org/10.1016/j.scitotenv.2012.03.065

CÓDIGO ALIMENTARIO ARGENTINO. 2012. Disponible en: http://www.anmat.gov.ar/alimentos/ codigoa/CAPITULO_XII.pdf [Acceso: 24 October 2018]

DE LA FOURNIÈRE, E. M., A. G. LEYVA, E. A. GAUTIER, M. I. LITTER. 2007. Treatment of phenylmercury salts by heterogeneous photocatalysis over TiO2. Chemosphere 69: 682-688. https://doi.org/10.1016/j.chemosphere.2007.05.042

DENG, C., D. ZHANG, X. PAN, F. CHANG, S. WANG. 2013. Toxic effects of mercury on PSI and PSII activities, membrane potential and transthylakoid proton gradient in Microsorium pteropus. J. Photoch. Photobio. B 127: 1-7. https://doi.org/10.1016/j.jphotobiol.2013.07.012

FEIJOO, C. S., R. J. LOMBARDO. 2007. Baseline water quality and macrophyte assemblages in Pampean streams: A regional approach. Water Res. 41: 1399- 1410. https://doi.org/10.1016/j.watres.2006.08.026

FORD, A., S. G. HAGEMANN, A. S. FOGLIATA, J. M. MILLER, A. MOL, P. J. DOYLE. 2015. Porphyry, epithermal, and orogenic gold prospectivity of Argentina. Ore Geol. Rev. 71: 655-672. https://doi.org/10.1016/j.oregeorev.2015.05.013

GARCÍA, S. I., G. BOVI MITRE, I. MORENO, M.

EIMAN GROSSI, A. DIGÓN, E. DE TITTO. 2003. Regional workshop on intoxications by plaguicides and harmonization in the collection of the information.

HE, Z., J. C. LI, H. ZHANG, M. MA. 2005. Different effects of calcium and lanthanum on the expression of phytochelatin synthase gene and cadmium absorption in Lactuca sativa. Plant Sci. 168: 309- 318. https://doi.org/10.1016/j.plantsci.2004.07.001

IAEA. 1980. Elemental Analysis of Biological Materials. Technical reports series No. 197.

KING, J. K., J. E. KOSTKA, M. E. FRISCHER, F. M. SAUNDERS, R. A. JAHNKE. 2001. A quantitative relationship that demonstrates mercury methylation rates in marine sediments are based on the community composition and activity of sulfate-reducing bacteria. Environ. Sci. Technol. 35: 2491-2496. https://doi.org/10.1021/es001813q

KUPPER, H., F. KUPPER, M. SPILLER. 1996. Environmental relevance of heavy metal substituted chlorophylls using the example of water plants. J. Exp. Bot. 47: 259-266. https://doi.org/10.1093/jxb/47.2.259

LEFEVRE H. W., J. C. OVERLEY, J. C. MCDONALD. 1987. Scanning transmission ion microscopy as it complements particle induced X-ray emission microscopy. Scanning Microsc. 1: 879-889.

LEFEVRE, H. W., R. M. S. SCHOFIELD, G. S. BENCH, G. J. F. LEGGE. 1991. STIM with energy loss contrast: an imaging modality unique to MeV ions. Nuc. Instr. Meth. Phys. Res. B 54: 363-370. https://doi.org/10.1016/0168-583X(91)95538-O

LLABADOR, Y. & P. MORETTO. 1996. Applications of nuclear microprobes in the life sciences, an efficient analytical technique for research in biology and medicine, World Scientific, Singapore, p. 65.

LOMONTE, C., Y. WANG, A. DORONILA, D. GREGORY, A. J. M. BAKER, R. SIEGELE, S. D. KOLEV. 2014. Study of the Spatial Distribution of Mercury in Roots of Vetiver Grass (Chrysopogon zizanioides) by Micro-Pixe Spectrometry. Int. J. Phytoremediat. 16: 1170-1182. https://doi.org/10.1080/15226514.2013.821453

LYUBENOVA, L., P. PONGRAC, K. VOGEL-MIKUŠ, G. KUKECMEZEK, P. VAVPETIČ, N. GRLJ, M. REGVAR, P. PELICON, P. SCHRÖDER. 2013. The fate of arsenic, cadmium and lead in Typha latifolia: A case study on the applicability of micro-PIXE in plant ionomics. J. Hazard. Mater. 248-249: 371-378. https://doi.org/10.1016/j.jhazmat.2013.01.023

273

Bol. Soc. Argent. Bot. 54 (2) 2019

MALAN H. L., J. MESJASZ-PRZYBYŁOWICZ, W. J. PRZYBYŁOWICZ, J. M. FARRANT, P. W. LINDER. 2012. Distribution patterns of the metal pollutants Cd and Ni in soybean seeds. Nucl. Instr. Meth. B 273: 157-160. https://doi.org/10.1016/j.nimb.2011.07.064

MANOHAR, D. M., K. ANOOP KRISHNAN, T. S. ANIRUDHAN. 2002. Removal of mercury (II) from aqueous solutions and chlor-alkali industry wastewater using 2-mercaptobenzimidazole-clay. Water Res. 36: 1609-1619. https://doi.org/10.1016/S0043-1354(01)00362-1

MATTINA, M. I., W. LANNUUCCI-BREGER, C. MUSANTE, J. C. WHITE. 2003. Concurrent uptake of heavy metals and persistent organic pollutants from soil. Environ. Pollut. 124: 375-378. https://doi.org/10.1016/S0269-7491(03)00060-5

MENDES GODINHO, R., J. RAIMUNDO, C. VALE, B. ANES, P. BRITO, L. C. ALVES, T. PINHEIRO. 2013. Micro-scale elemental partition in tissues of the aquatic plant Lemna minor L. exposed to highway drainage water. Nucl. Instrum. Meth. B 306: 150-152. https://doi.org/10.1016/j.nimb.2012.10.032

M E S J A S Z - P R Z Y B Y Ł O W I C Z , J . , W. J . PRZYBYŁOWICZ. 2002. Micro-PIXE in plant sciences: Present status and perspectives. Nucl. Instrum. Meth. B 189: 470-481. https://doi.org/10.1016/S0168-583X(01)01127-2

MIRETZKY, P., A. SARALEGUI, A. FERNÁNDEZ CIRELLI. 2004. Aquatic macrophytes potential for the simultaneous removal of heavy metals (Buenos Aires, Argentina). Chemosphere 57: 997-1005. https://doi.org/10.1016/j.chemosphere.2004.07.024

MISHRA, K. V., B. D. TRIPATHI, K. H. KIM. 2009. Removal and accumulation of mercury by aquatic macrophytes from an open cast coal mine effluent. J. Hazard. Mater. 172: 749-754. https://doi.org/10.1016/j.jhazmat.2009.07.059

MÓDENES, A. N., F. R. ESPINOZA-QUIÑONES, G. H. F. SANTOS, C. E. BORBA, M. A. RIZZUTTO. 2013. Assessment of metal sorption mechanisms by aquatic macrophytes using PIXE analysis. J. Hazard. Mater. 261: 148-154. https://doi.org/10.1016/j.jhazmat.2013.07.020

NRIAGU, J.O. (ed.). 1979. The Biogeochemistry of Mercury in the Environment. Elsevier North Holland Biomedical, Amsterdam.

PANDEY, S., R. K. ASTHANA, A. M. KAYASTHA, N. SINGH, S. P. SINGH. 1999. Metal Uptake and Thiol Production in Spirodela polyrhiza (L.) SP20. J. Plant Physiol. 154: 634-640. https://doi.org/10.1016/S0176-1617(99)80238-7

PORATH D. & J. POLLOCK. 1982. Ammonia stripping by duckweed and its feasibility in circulating aquaculture. Aquat. Bot. 13: 125-131. https://doi.org/10.1016/0304-3770(82)90046-8

RAHMAN, M. A., H. HASEGAWA. 2011. Aquatic arsenic: Phytoremediation using floating macrophytes. Chemosphere 83: 633-646. https://doi.org/10.1016/j.chemosphere.2011.02.045

RASCIO, N. & F. NAVARI-IZZO. 2011. Heavy metal hyperaccumulating plants: How and why do they do it? And what makes them so interesting? Plant Sci.

180:169-181. https://doi.org/10.1016/j.plantsci.2010.08.016

ROSSINI, E. & E. W. KNAPP. 2017. Protonation equilibria of transition metal complexes: From model systems toward the Mn-complexin photosystem II. Coord. Chem. Rev. 345: 16-30. https://doi.org/10.1016/j.ccr.2017.02.017

SERPONE, N., E. BORGARELLO, E. PELIZZETTI. 1988. Photoreduction and photodegradation of inorganic pollutants: II. Selective reduction and recovery ofAu, Pt, Pd, Rh, Hg, and Pb. In: Schiavello, M. (Ed.), Photocatalysis and Environment. Kluwer Academic Publishers, Dordrecht, pp. 527-565.

SOUTHWORTH-DAVIESA, R. J., K. LEATHA, G. W. GRIME, E. F. GARMANA. 2007. The Characterisation of a Contaminant-free Support Film for MicroPIXE Analysis of Biological Samples. Proceedings of the XI International Conference on PIXE and its Analytical Applications Puebla, Mexico, May 25-29. Disponible en: http://www. fisica.unam.mx/pixe2007/Downloads/Proceedings/ PDF_Files/PIXE2007-C-2.pdf [Acceso: 4 February 2019]

STOLIAR, P., A. J. KREINER, M. E. DEBRAY, M. E. CARABALLO, A. A. VALDA, J. DAVIDSON, M. DAVIDSON, J. M. KESQUE, H. SOMACAL, H. DIPAOLO, A. A. BURLON, M. J. OZAFRÁN, M. E. VÁZQUEZ, D. MINSKY, E. M. HEBER, V. A. TRIVILLIN, A. E. SCHWINT. 2004. Microdistributions of prospective BNCT- compound CuTCPH in tissue sections with a heavy ion microbeam. Appl. Radiat. Isot. 61: 771-774. https://doi.org/10.1016/j.apradiso.2004.05.062

SRIVASTAV, R. K., S. K. GUPTA, K. D. P. NIGAM, P. VASUDEVAN. 1994. Treatment of chromium and nickel in wastewater by using aquatic plants. Water Res. 28: 1631-1638. https://doi.org/10.1016/0043-1354(94)90231-3

TIAN, S., L. LU, J. ZHANG, K. WANG, P. BROWN, Z. HE, J. LIANG, X. YANG. 2011. Calcium protects roots of Sedum alfredii H. against cadmium-induced oxidative stress. Chemosphere 84: 63-69. https://doi.org/10.1016/j.chemosphere.2011.02.054

VARDANYAN, L. G., B. S. INGOLE. 2006. Studies on heavy metal accumulation in aquatic macrophytes from Sevan (Armenia) and Carambolim (India) lake systems. Environ. Int. 32: 208-218. https://doi.org/10.1016/j.envint.2005.08.013

274

E. M. de la Fournière et al. - Hyperaccumulation of Hg by S. intermedia evaluated by microPIXE

VOGEL-MIKUŠ, K. P. PONGRAC, P. KUMP, M. NEČEMER, J. SIMČIČ, P. PELICON, M. BUDNAR, B. POVH, M. REGVAR. 2007. Localisation and quantification of elements within seeds of Cd/Zn hyperaccumulator Thlaspi praecox by micro-PIXE. Environ. Pollut. 147: 50-59. https://doi.org/10.1016/j.envpol.2006.08.026

VOGEL-MIKUŠ, K., P. PONGRAC, P. PELICON, P. VAVPETIČ, B. POVH, H. BOTHE AND

M.REGVAR. 2009. Micro-PIXE analysis for localization and quantification of elements in roots of mycorrhizal plants. In: Soil Biology 18, chapter 14, pp. 227-242, eds. Varma A. and Amit Kharkwal A.C., Springer, Berlin, Heidelberg.

WANG, Y. D., J. MESJASZ-PRZYBYŁOWICZ, G. TYLKO, A. D. BARNABAS, W. J. PRZYBYŁOWICZ. 2013. Micro-PIXE analyses of frozen-hydrated semi-thick biological sections.

Nucl. Instr. Meth. B 306: 134-139. https://doi.org/10.1016/j.nimb.2012.12.051

WITKOWSKI, E. T. F., I. M. WEIERSBYE-

WITKOWSKI, W. J. PRZYBYŁOWICZ, J. MESJASZ-PRZYBYŁOWICZ. 1997. Nuclear microprobe studies of elemental distributions in dormant seeds of Burkea africana. Nucl. Instr. Meth. B 130: 381-387. https://doi.org/10.1016/S0168-583X(97)00231-0

YIN, Y., B. CHEN, Y. MAO, T. WANG, J. LIU, Y. CAI, G. JIANG. 2012. Possible alkylation of inorganic Hg(II) by photochemical processes in the environment. Chemosphere 88: 8-16. https://doi.org/10.1016/j.chemosphere.2012.01.006

YUH-SHAN, H. 2004. Citation review of Lagergren kinetic rate equation on adsorption Reactions. Scientometrics 59: 171-177. https://doi.org/10.1023/B:SCIE.0000013305.99473.cf

275